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Protein

Pre-mRNA-processing factor 6

Gene

Prpf6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 6
Alternative name(s):
PRP6 homolog
U5 snRNP-associated 102 kDa protein
Short name:
U5-102 kDa protein
Gene namesi
Name:Prpf6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1307103. Prpf6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Pre-mRNA-processing factor 6PRO_0000413096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431PhosphoserineCombined sources
Modified residuei180 – 1801PhosphothreonineBy similarity
Modified residuei266 – 2661PhosphothreonineBy similarity
Modified residuei275 – 2751PhosphothreonineBy similarity
Modified residuei279 – 2791PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA1A5S1.
PeptideAtlasiA1A5S1.
PRIDEiA1A5S1.

PTM databases

iPTMnetiA1A5S1.

Expressioni

Gene expression databases

GenevisibleiA1A5S1. RN.

Interactioni

Subunit structurei

Associates with the U5 snRNP particle. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, LSm proteins LSm2-8 and Sm proteins (By similarity). Interacts with ARAF1. Identified in the spliceosome C complex (By similarity). Interacts with AR and NR3C1, but not ESR1, independently of the presence of hormones (By similarity).By similarity

Protein-protein interaction databases

BioGridi265839. 1 interaction.
STRINGi10116.ENSRNOP00000021723.

Structurei

3D structure databases

ProteinModelPortaliA1A5S1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati384 – 41633HAT 1Add
BLAST
Repeati418 – 44427HAT 2Add
BLAST
Repeati445 – 47632HAT 3Add
BLAST
Repeati554 – 58633HAT 4Add
BLAST
Repeati588 – 62033HAT 5Add
BLAST
Repeati622 – 65433HAT 6Add
BLAST
Repeati689 – 72133HAT 7Add
BLAST
Repeati723 – 75533HAT 8Add
BLAST
Repeati855 – 88733HAT 9Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi67 – 726Poly-Asp

Sequence similaritiesi

Contains 9 HAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0495. Eukaryota.
ENOG410XRKD. LUCA.
GeneTreeiENSGT00550000075016.
HOGENOMiHOG000116247.
HOVERGENiHBG023330.
InParanoidiA1A5S1.
KOiK12855.
OMAiVFERQQG.
OrthoDBiEOG7RV9FB.
PhylomeDBiA1A5S1.
TreeFamiTF105743.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR003107. HAT.
IPR010491. PRP1_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 13 hits.
SM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1A5S1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP
60 70 80 90 100
PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE
110 120 130 140 150
ADAIYAALDK RMDERRKERR EQREKEEIEK YRMERPKIQQ QFSDLKRKLA
160 170 180 190 200
EVTEEEWLSI PEVGDARNKR QRNPRYEKLT PVPDSFFAKH LQTGENHTSV
210 220 230 240 250
DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM RKIGQARNTL
260 270 280 290 300
MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS
310 320 330 340 350
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA
360 370 380 390 400
RLQPGDTAKA VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV
410 420 430 440 450
PNSVRLWKAA VELEEPEDAR IMLSRAVECC PTSVELWLAL ARLETYENAR
460 470 480 490 500
KVLNKARENI PTDRHIWITA AKLEEANGNT QMVEKIIDRA ITSLRANGVE
510 520 530 540 550
INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK HTWMEDADSC
560 570 580 590 600
VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA
610 620 630 640 650
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK
660 670 680 690 700
LESENNEYER ARRLLAKARS SAPTARVFMK SVKLEWVLGN ITAAQELCEE
710 720 730 740 750
ALRHYEDFPK LWMMKGQIEE QGELMERARE AYNQGLKKCP HSTPLWLLLS
760 770 780 790 800
RLEEKIGQLT RARAILEKSR LKNPKNPGLW LESVRLEYRA GLKNIANTLM
810 820 830 840 850
AKALQECPNS GILWSEAVFL EARPQRKTKS VDALKKCEHD PHVLLAVAKL
860 870 880 890 900
FWSERKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR
910 920 930 940
CENAEPRHGE LWCAVSKDIT NWQRKIGEIL VLVAARIKNT F
Length:941
Mass (Da):106,764
Last modified:January 23, 2007 - v1
Checksum:i12F1405BD6433254
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474066 Genomic DNA. Translation: EDL88695.1.
BC128779 mRNA. Translation: AAI28780.1.
RefSeqiNP_001073234.1. NM_001079766.1.
UniGeneiRn.17062.

Genome annotation databases

EnsembliENSRNOT00000083423; ENSRNOP00000073536; ENSRNOG00000051498.
GeneIDi366276.
KEGGirno:366276.
UCSCiRGD:1307103. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474066 Genomic DNA. Translation: EDL88695.1.
BC128779 mRNA. Translation: AAI28780.1.
RefSeqiNP_001073234.1. NM_001079766.1.
UniGeneiRn.17062.

3D structure databases

ProteinModelPortaliA1A5S1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi265839. 1 interaction.
STRINGi10116.ENSRNOP00000021723.

PTM databases

iPTMnetiA1A5S1.

Proteomic databases

PaxDbiA1A5S1.
PeptideAtlasiA1A5S1.
PRIDEiA1A5S1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000083423; ENSRNOP00000073536; ENSRNOG00000051498.
GeneIDi366276.
KEGGirno:366276.
UCSCiRGD:1307103. rat.

Organism-specific databases

CTDi24148.
RGDi1307103. Prpf6.

Phylogenomic databases

eggNOGiKOG0495. Eukaryota.
ENOG410XRKD. LUCA.
GeneTreeiENSGT00550000075016.
HOGENOMiHOG000116247.
HOVERGENiHBG023330.
InParanoidiA1A5S1.
KOiK12855.
OMAiVFERQQG.
OrthoDBiEOG7RV9FB.
PhylomeDBiA1A5S1.
TreeFamiTF105743.

Enzyme and pathway databases

ReactomeiR-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

PROiA1A5S1.

Gene expression databases

GenevisibleiA1A5S1. RN.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR003107. HAT.
IPR010491. PRP1_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 13 hits.
SM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
PROSITEiPS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway.
    Tissue: Heart.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP6_RAT
AccessioniPrimary (citable) accession number: A1A5S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.