Tyrosine-protein kinase yes - Danio rerio (Zebrafish)

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A1A5H8 (YES_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 63. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase yes
EC=2.7.10.2

Alternative name(s):
p61-Yes

Gene names

Name:	yes1
Synonyms:	yes

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, differentiation, G2/M progression and cytokinesis By similarity. Required for convergent extension cell movements during gastrulation, acting with fyna via rhoa. May be required for epiboly to occur, possibly through its effects in calcium signaling. During embryonic development, phosphorylates ptk2.1/fak. Ref.1 Ref.4
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Cell membrane. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytosol By similarity Ref.4.
Tissue specificity	Widely expressed. Ref.1
Developmental stage	Expressed in unfertilized eggs (at protein level). Most highly enriched in the cortex of the newly fertilized egg. Becomes concentrated in the blastodisc by 30 minutes post-insemination and remains distributed among all regions of the embryo, excluding yolk. In the pharyngula stage (24 hours post-fertilization), observed all over the embryo, with highest levels in the eyes and central nervous system and somites (at protein level). Ref.4
Post-translational modification	Autophosphorylation at Tyr-429 maintains enzyme activity By similarity. Palmitoylation at Cys-3 promotes membrane localization By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Membrane
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	convergent extension involved in gastrulation Inferred from genetic interaction Ref.1. Source: ZFIN protein autophosphorylation Inferred from direct assay Ref.4. Source: ZFIN regulation of protein kinase activity Inferred from genetic interaction PubMed 17611617 PubMed 18550893. Source: ZFIN
Cellular_component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from direct assay Ref.4. Source: ZFIN
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 546

545

Tyrosine-protein kinase yes

PRO_0000418881

Regions

Domain

94 – 155

SH3

Domain

161 – 258

SH2

Domain

280 – 533

254

Protein kinase

Nucleotide binding

286 – 294

ATP By similarity

Sites

Active site

399

Proton acceptor By similarity

Binding site

308

ATP By similarity

Amino acid modifications

Modified residue

429

Phosphotyrosine; by autocatalysis By similarity

Modified residue

540

Phosphotyrosine; by CSK By similarity

Lipidation

N-myristoyl glycine By similarity

Lipidation

S-palmitoyl cysteine; in membrane form By similarity

Experimental info

Sequence conflict

T → S in AAN73265. Ref.4

Sequence conflict

S → P in AAN73265. Ref.4

Sequence conflict

132

W → C in AAN73265. Ref.4

Sequence conflict

514 – 515

KE → NG in CAF06180. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

A1A5H8 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 7067657BA2B8F420
Show »

FASTA

546

61,269

        10         20         30         40         50         60 
MGCVKSKEDK GPTQKYRPDP TNPTPGSHMG LYGPDPTQMG QSPALKGPTN NYNSRSSGLT 

        70         80         90        100        110        120 
PFGGSSSVIT PFGGASSSFS TVAVNNPFPG VVTGGVTFFV ALYDYEARTS DDLSFSKGDR 

       130        140        150        160        170        180 
FQIINNTEGD WWEARSINTG QKGYIPSNYV APADSIQAEE WYFGKMGRKD AERLLLLPGN 

       190        200        210        220        230        240 
QRGTFLVRES ETTKGAYSLS IRDWDEMKGD NVKHYKIRKL DSGGYYITTR AQFDTLQKLV 

       250        260        270        280        290        300 
KHYTEHADGL CYRLTTVCPT VKPQTQGLAK DAWEIPRESL RLELKLGQGC FGEVWMGTWN 

       310        320        330        340        350        360 
GTTKVAIKTL KPGTMSPEAF LQEAQIMKKL RHDKLVPLYA VVSEEPIYIV TEYMGKGSLL 

       370        380        390        400        410        420 
DFLKEGEGKY LKLPQLVDMA AQIADGMAFI ERMNYIHRDL RAANILVGDN LVCKIADFGL 

       430        440        450        460        470        480 
ARLIEDNEYT ARQGAKFPIK WTAPEAALYG RFTIKSDVWS FGILLTELVT KGRVPYPGMV 

       490        500        510        520        530        540 
NREVLEQVER GYRMPCPQGC PESLHEMMRL CWKKEPDERP TFEYIQSFLE DYFTATEPQY 


QPGDNL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fyn/Yes and non-canonical Wnt signalling converge on RhoA in vertebrate gastrulation cell movements." Jopling C., den Hertog J. EMBO Rep. 6:426-431(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"The genomic sequence of Danio rerio." The Danio rerio Sequencing Project at the Sanger Institute Submitted (JUL-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[3]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Role of Yes kinase during early zebrafish development." Tsai W.B., Zhang X., Sharma D., Wu W., Kinsey W.H. Dev. Biol. 277:129-141(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-135, FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AUTOPHOSPHORYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ620749 mRNA. Translation: CAF06180.1. BX927088 Genomic DNA. Translation: CAN88236.1. BC128657 mRNA. Translation: AAI28658.1. AY157872 mRNA. Translation: AAN73265.1.
IPI	IPI00487427.
RefSeq	NP_001013288.2. NM_001013270.3.
UniGene	Dr.75637.
3D structure databases
ProteinModelPortal	A1A5H8.
SMR	A1A5H8. Positions 96-546.
ModBase	Search...
Protein-protein interaction databases
STRING	7955.ENSDARP00000009659.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000027090; ENSDARP00000009659; ENSDARG00000005941.
GeneID	407620.
KEGG	dre:407620.
Organism-specific databases
CTD	7525.
ZFIN	ZDB-GENE-050126-1. yes1.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00620000087702.
HOGENOM	HOG000233858.
HOVERGEN	HBG008761.
InParanoid	A1A5H8.
KO	K05705.
OMA	IKYRTEN.
OrthoDB	EOG4KKZ2S.
Gene expression databases
Bgee	A1A5H8.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20818497.

Entry information

Entry name

YES_DANRE

Accession

Primary (citable) accession number: A1A5H8
Secondary accession number(s): Q6EWH1, Q8AXW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents