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A1A5H8

- YES_DANRE

UniProt

A1A5H8 - YES_DANRE

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Protein
Tyrosine-protein kinase yes
Gene
yes1, yes
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, differentiation, G2/M progression and cytokinesis By similarity. Required for convergent extension cell movements during gastrulation, acting with fyna via rhoa. May be required for epiboly to occur, possibly through its effects in calcium signaling. During embryonic development, phosphorylates ptk2.1/fak.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei308 – 3081ATP By similarity
Active sitei399 – 3991Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi286 – 2949ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein tyrosine kinase activity Source: ZFIN

GO - Biological processi

  1. convergent extension involved in gastrulation Source: ZFIN
  2. peptidyl-tyrosine phosphorylation Source: GOC
  3. protein autophosphorylation Source: ZFIN
  4. regulation of protein kinase activity Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_174907. Regulation of KIT signaling.
REACT_181648. Signaling by SCF-KIT.
REACT_221534. FCGR activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase yes (EC:2.7.10.2)
Alternative name(s):
p61-Yes
Gene namesi
Name:yes1
Synonyms:yes
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 2

Organism-specific databases

ZFINiZDB-GENE-050126-1. yes1.

Subcellular locationi

Cell membrane. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytosol By similarity 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 546545Tyrosine-protein kinase yes
PRO_0000418881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Lipidationi3 – 31S-palmitoyl cysteine; in membrane form By similarity
Modified residuei429 – 4291Phosphotyrosine; by autocatalysis By similarity
Modified residuei540 – 5401Phosphotyrosine; by CSK By similarity

Post-translational modificationi

Autophosphorylation at Tyr-429 maintains enzyme activity By similarity.
Palmitoylation at Cys-3 promotes membrane localization By similarity.

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed in unfertilized eggs (at protein level). Most highly enriched in the cortex of the newly fertilized egg. Becomes concentrated in the blastodisc by 30 minutes post-insemination and remains distributed among all regions of the embryo, excluding yolk. In the pharyngula stage (24 hours post-fertilization), observed all over the embryo, with highest levels in the eyes and central nervous system and somites (at protein level).1 Publication

Gene expression databases

BgeeiA1A5H8.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000009659.

Structurei

3D structure databases

ProteinModelPortaliA1A5H8.
SMRiA1A5H8. Positions 96-546.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 15562SH3
Add
BLAST
Domaini161 – 25898SH2
Add
BLAST
Domaini280 – 533254Protein kinase
Add
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00620000087702.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiA1A5H8.
KOiK05705.
OMAiIKYRTEN.
OrthoDBiEOG7GTT2V.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1A5H8-1 [UniParc]FASTAAdd to Basket

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MGCVKSKEDK GPTQKYRPDP TNPTPGSHMG LYGPDPTQMG QSPALKGPTN    50
NYNSRSSGLT PFGGSSSVIT PFGGASSSFS TVAVNNPFPG VVTGGVTFFV 100
ALYDYEARTS DDLSFSKGDR FQIINNTEGD WWEARSINTG QKGYIPSNYV 150
APADSIQAEE WYFGKMGRKD AERLLLLPGN QRGTFLVRES ETTKGAYSLS 200
IRDWDEMKGD NVKHYKIRKL DSGGYYITTR AQFDTLQKLV KHYTEHADGL 250
CYRLTTVCPT VKPQTQGLAK DAWEIPRESL RLELKLGQGC FGEVWMGTWN 300
GTTKVAIKTL KPGTMSPEAF LQEAQIMKKL RHDKLVPLYA VVSEEPIYIV 350
TEYMGKGSLL DFLKEGEGKY LKLPQLVDMA AQIADGMAFI ERMNYIHRDL 400
RAANILVGDN LVCKIADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG 450
RFTIKSDVWS FGILLTELVT KGRVPYPGMV NREVLEQVER GYRMPCPQGC 500
PESLHEMMRL CWKKEPDERP TFEYIQSFLE DYFTATEPQY QPGDNL 546
Length:546
Mass (Da):61,269
Last modified:January 23, 2007 - v1
Checksum:i7067657BA2B8F420
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211T → S in AAN73265. 1 Publication
Sequence conflicti56 – 561S → P in AAN73265. 1 Publication
Sequence conflicti132 – 1321W → C in AAN73265. 1 Publication
Sequence conflicti514 – 5152KE → NG in CAF06180. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ620749 mRNA. Translation: CAF06180.1.
BX927088 Genomic DNA. Translation: CAN88236.1.
BC128657 mRNA. Translation: AAI28658.1.
AY157872 mRNA. Translation: AAN73265.1.
RefSeqiNP_001013288.2. NM_001013270.3.
UniGeneiDr.75637.

Genome annotation databases

EnsembliENSDART00000027090; ENSDARP00000009659; ENSDARG00000005941.
GeneIDi407620.
KEGGidre:407620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ620749 mRNA. Translation: CAF06180.1 .
BX927088 Genomic DNA. Translation: CAN88236.1 .
BC128657 mRNA. Translation: AAI28658.1 .
AY157872 mRNA. Translation: AAN73265.1 .
RefSeqi NP_001013288.2. NM_001013270.3.
UniGenei Dr.75637.

3D structure databases

ProteinModelPortali A1A5H8.
SMRi A1A5H8. Positions 96-546.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000009659.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000027090 ; ENSDARP00000009659 ; ENSDARG00000005941 .
GeneIDi 407620.
KEGGi dre:407620.

Organism-specific databases

CTDi 7525.
ZFINi ZDB-GENE-050126-1. yes1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00620000087702.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi A1A5H8.
KOi K05705.
OMAi IKYRTEN.
OrthoDBi EOG7GTT2V.
TreeFami TF351634.

Enzyme and pathway databases

Reactomei REACT_174907. Regulation of KIT signaling.
REACT_181648. Signaling by SCF-KIT.
REACT_221534. FCGR activation.

Miscellaneous databases

NextBioi 20818497.
PROi A1A5H8.

Gene expression databases

Bgeei A1A5H8.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Fyn/Yes and non-canonical Wnt signalling converge on RhoA in vertebrate gastrulation cell movements."
    Jopling C., den Hertog J.
    EMBO Rep. 6:426-431(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  3. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Role of Yes kinase during early zebrafish development."
    Tsai W.B., Zhang X., Sharma D., Wu W., Kinsey W.H.
    Dev. Biol. 277:129-141(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-135, FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AUTOPHOSPHORYLATION.

Entry informationi

Entry nameiYES_DANRE
AccessioniPrimary (citable) accession number: A1A5H8
Secondary accession number(s): Q6EWH1, Q8AXW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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