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A1A5H8

- YES_DANRE

UniProt

A1A5H8 - YES_DANRE

Protein

Tyrosine-protein kinase yes

Gene

yes1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, differentiation, G2/M progression and cytokinesis By similarity. Required for convergent extension cell movements during gastrulation, acting with fyna via rhoa. May be required for epiboly to occur, possibly through its effects in calcium signaling. During embryonic development, phosphorylates ptk2.1/fak.By similarity2 Publications

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei308 – 3081ATPPROSITE-ProRule annotation
    Active sitei399 – 3991Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi286 – 2949ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein tyrosine kinase activity Source: ZFIN

    GO - Biological processi

    1. convergent extension involved in gastrulation Source: ZFIN
    2. peptidyl-tyrosine phosphorylation Source: GOC
    3. protein autophosphorylation Source: ZFIN
    4. regulation of protein kinase activity Source: ZFIN

    Keywords - Molecular functioni

    Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_174907. Regulation of KIT signaling.
    REACT_181648. Signaling by SCF-KIT.
    REACT_221534. FCGR activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase yes (EC:2.7.10.2)
    Alternative name(s):
    p61-Yes
    Gene namesi
    Name:yes1
    Synonyms:yes
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 2

    Organism-specific databases

    ZFINiZDB-GENE-050126-1. yes1.

    Subcellular locationi

    Cell membrane 1 Publication. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 546545Tyrosine-protein kinase yesPRO_0000418881Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycineBy similarity
    Lipidationi3 – 31S-palmitoyl cysteine; in membrane formBy similarity
    Modified residuei429 – 4291Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei540 – 5401Phosphotyrosine; by CSKBy similarity

    Post-translational modificationi

    Autophosphorylation at Tyr-429 maintains enzyme activity.By similarity
    Palmitoylation at Cys-3 promotes membrane localization.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate, Palmitate, Phosphoprotein

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Developmental stagei

    Expressed in unfertilized eggs (at protein level). Most highly enriched in the cortex of the newly fertilized egg. Becomes concentrated in the blastodisc by 30 minutes post-insemination and remains distributed among all regions of the embryo, excluding yolk. In the pharyngula stage (24 hours post-fertilization), observed all over the embryo, with highest levels in the eyes and central nervous system and somites (at protein level).1 Publication

    Gene expression databases

    BgeeiA1A5H8.

    Interactioni

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000009659.

    Structurei

    3D structure databases

    ProteinModelPortaliA1A5H8.
    SMRiA1A5H8. Positions 96-546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini94 – 15562SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini161 – 25898SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini280 – 533254Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00620000087702.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    InParanoidiA1A5H8.
    KOiK05705.
    OMAiIKYRTEN.
    OrthoDBiEOG7GTT2V.
    TreeFamiTF351634.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1A5H8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCVKSKEDK GPTQKYRPDP TNPTPGSHMG LYGPDPTQMG QSPALKGPTN    50
    NYNSRSSGLT PFGGSSSVIT PFGGASSSFS TVAVNNPFPG VVTGGVTFFV 100
    ALYDYEARTS DDLSFSKGDR FQIINNTEGD WWEARSINTG QKGYIPSNYV 150
    APADSIQAEE WYFGKMGRKD AERLLLLPGN QRGTFLVRES ETTKGAYSLS 200
    IRDWDEMKGD NVKHYKIRKL DSGGYYITTR AQFDTLQKLV KHYTEHADGL 250
    CYRLTTVCPT VKPQTQGLAK DAWEIPRESL RLELKLGQGC FGEVWMGTWN 300
    GTTKVAIKTL KPGTMSPEAF LQEAQIMKKL RHDKLVPLYA VVSEEPIYIV 350
    TEYMGKGSLL DFLKEGEGKY LKLPQLVDMA AQIADGMAFI ERMNYIHRDL 400
    RAANILVGDN LVCKIADFGL ARLIEDNEYT ARQGAKFPIK WTAPEAALYG 450
    RFTIKSDVWS FGILLTELVT KGRVPYPGMV NREVLEQVER GYRMPCPQGC 500
    PESLHEMMRL CWKKEPDERP TFEYIQSFLE DYFTATEPQY QPGDNL 546
    Length:546
    Mass (Da):61,269
    Last modified:January 23, 2007 - v1
    Checksum:i7067657BA2B8F420
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti21 – 211T → S in AAN73265. (PubMed:15572145)Curated
    Sequence conflicti56 – 561S → P in AAN73265. (PubMed:15572145)Curated
    Sequence conflicti132 – 1321W → C in AAN73265. (PubMed:15572145)Curated
    Sequence conflicti514 – 5152KE → NG in CAF06180. (PubMed:15815683)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ620749 mRNA. Translation: CAF06180.1.
    BX927088 Genomic DNA. Translation: CAN88236.1.
    BC128657 mRNA. Translation: AAI28658.1.
    AY157872 mRNA. Translation: AAN73265.1.
    RefSeqiNP_001013288.2. NM_001013270.3.
    UniGeneiDr.75637.

    Genome annotation databases

    EnsembliENSDART00000027090; ENSDARP00000009659; ENSDARG00000005941.
    GeneIDi407620.
    KEGGidre:407620.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ620749 mRNA. Translation: CAF06180.1 .
    BX927088 Genomic DNA. Translation: CAN88236.1 .
    BC128657 mRNA. Translation: AAI28658.1 .
    AY157872 mRNA. Translation: AAN73265.1 .
    RefSeqi NP_001013288.2. NM_001013270.3.
    UniGenei Dr.75637.

    3D structure databases

    ProteinModelPortali A1A5H8.
    SMRi A1A5H8. Positions 96-546.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000009659.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000027090 ; ENSDARP00000009659 ; ENSDARG00000005941 .
    GeneIDi 407620.
    KEGGi dre:407620.

    Organism-specific databases

    CTDi 7525.
    ZFINi ZDB-GENE-050126-1. yes1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00620000087702.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    InParanoidi A1A5H8.
    KOi K05705.
    OMAi IKYRTEN.
    OrthoDBi EOG7GTT2V.
    TreeFami TF351634.

    Enzyme and pathway databases

    Reactomei REACT_174907. Regulation of KIT signaling.
    REACT_181648. Signaling by SCF-KIT.
    REACT_221534. FCGR activation.

    Miscellaneous databases

    NextBioi 20818497.
    PROi A1A5H8.

    Gene expression databases

    Bgeei A1A5H8.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fyn/Yes and non-canonical Wnt signalling converge on RhoA in vertebrate gastrulation cell movements."
      Jopling C., den Hertog J.
      EMBO Rep. 6:426-431(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    2. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    3. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Role of Yes kinase during early zebrafish development."
      Tsai W.B., Zhang X., Sharma D., Wu W., Kinsey W.H.
      Dev. Biol. 277:129-141(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-135, FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AUTOPHOSPHORYLATION.

    Entry informationi

    Entry nameiYES_DANRE
    AccessioniPrimary (citable) accession number: A1A5H8
    Secondary accession number(s): Q6EWH1, Q8AXW6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3