Reviewed, UniProtKB/Swiss-Prot A1A5B4 (ANO9_HUMAN)

Names and origin

Protein names

Recommended name:
    Anoctamin-9
Alternative name(s):
    Transmembrane protein 16J
    Tumor protein p53-inducible protein 5
    p53-induced gene 5 protein

Gene names

Name:	ANO9
Synonyms:	PIG5, TMEM16J, TP53I5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	782 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May act as a calcium-activated chloride channel.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the anoctamin family.
Sequence caution	The sequence BAB84914.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	Calcium Chloride
Molecular function	Chloride channel Ionic channel
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW chloride ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A1A5B4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A1A5B4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-299: Missing.
     300-305: VWDEEQ → MPAVSE

Isoform 3 (identifier: A1A5B4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     156-186: GEGRLKKTWARWRHMFREQPVDEIRNYFGEK → VRGGPAWRGPWGGTLGWGLSLSVTRARGRDA
     187-782: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 782	782	Anoctamin-9		PRO_0000289329
Regions
	Transmembrane	199 – 219	21	Potential
	Transmembrane	265 – 285	21	Potential
	Transmembrane	332 – 352	21	Potential
	Transmembrane	374 – 394	21	Potential
	Transmembrane	424 – 444	21	Potential
	Transmembrane	553 – 573	21	Potential
	Transmembrane	605 – 625	21	Potential
	Transmembrane	704 – 724	21	Potential
Natural variations
	Alternative sequence	1 – 299	299	Missing in isoform 2.		VSP_036489
	Alternative sequence	156 – 186	31	GEGRL…YFGEK → VRGGPAWRGPWGGTLGWGLS LSVTRARGRDA in isoform 3.		VSP_036490
	Alternative sequence	187 – 782	596	Missing in isoform 3.		VSP_036491
	Alternative sequence	300 – 305	6	VWDEEQ → MPAVSE in isoform 2.		VSP_036492
	Natural variant	93	1	F → L: dbSNP rs7395065. Ref.2 Ref.4		VAR_054621
	Natural variant	391	1	I → V: dbSNP rs10794324. Ref.2 Ref.4 Ref.1		VAR_032617
	Natural variant	399	1	C → R: dbSNP rs10794323. Ref.2 Ref.4 Ref.1		VAR_032618

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: C319076A5C1433FF	FASTA	782	90,367
	10 20 30 40 50 60 MQGEESLRIL VEPEGDSFPL MEISTCETEA SEQWDYVLVA QRHTQRDPRQ ARQQQFLEEL 70 80 90 100 110 120 RRKGFHIKVI RDQKQVFFGI RADNSVFGLY RTFLLEPEGP APHAELAAPT TIPVTTSLRI 130 140 150 160 170 180 RIVNFVVMNN KTSAGETFED LMKDGVFEAR FPLHKGEGRL KKTWARWRHM FREQPVDEIR 190 200 210 220 230 240 NYFGEKVALY FVWLGWYTYM LVPAALTGLL VFLSGFSLFE ASQISKEICE AHDILMCPLG 250 260 270 280 290 300 DHSRRYQRLS ETCTFAKLTH LFDNDGTVVF AIFMALWATV FLEIWKRQRA RVVLHWDLYV 310 320 330 340 350 360 WDEEQEEMAL QLINCPDYKL RPYQHSYLRS TVILVLTLLM ICLMIGMAHV LVVYRVLASA 370 380 390 400 410 420 LFSSSAVPFL EEQVTTAVVV TGALVHYVTI IIMTKINRCV ALKLCDFEMP RTFSERESRF 430 440 450 460 470 480 TIRFFTLQFF THFSSLIYIA FILGRINGHP GKSTRLAGLW KLEECHASGC MMDLFVQMAI 490 500 510 520 530 540 IMGLKQTLSN CVEYLVPWVT HKCRSLRASE SGHLPRDPEL RDWRRNYLLN PVNTFSLFDE 550 560 570 580 590 600 FMEMMIQYGF TTIFVAAFPL APLLALFSNL VEIRLDAIKM VWLQRRLVPR KAKDIGTWLQ 610 620 630 640 650 660 VLETIGVLAV IANGMVIAFT SEFIPRVVYK YRYSPCLKEG NSTVDCLKGY VNHSLSVFHT 670 680 690 700 710 720 KDFQDPDGIE GSENVTLCRY RDYRNPPDYN FSEQFWFLLA IRLAFVILFE HVALCIKLIA 730 740 750 760 770 780 AWFVPDIPQS VKNKVLEVKY QRLREKMWHG RQRLGGVGAG SRPPMPAHPT PASIFSARST DV
	Isoform 2. Checksum: 8474676D70D18C37	FASTA	483	55,280
	10 20 30 40 50 60 MPAVSEEEMA LQLINCPDYK LRPYQHSYLR STVILVLTLL MICLMIGMAH VLVVYRVLAS 70 80 90 100 110 120 ALFSSSAVPF LEEQVTTAVV VTGALVHYVT IIIMTKINRC VALKLCDFEM PRTFSERESR 130 140 150 160 170 180 FTIRFFTLQF FTHFSSLIYI AFILGRINGH PGKSTRLAGL WKLEECHASG CMMDLFVQMA 190 200 210 220 230 240 IIMGLKQTLS NCVEYLVPWV THKCRSLRAS ESGHLPRDPE LRDWRRNYLL NPVNTFSLFD 250 260 270 280 290 300 EFMEMMIQYG FTTIFVAAFP LAPLLALFSN LVEIRLDAIK MVWLQRRLVP RKAKDIGTWL 310 320 330 340 350 360 QVLETIGVLA VIANGMVIAF TSEFIPRVVY KYRYSPCLKE GNSTVDCLKG YVNHSLSVFH 370 380 390 400 410 420 TKDFQDPDGI EGSENVTLCR YRDYRNPPDY NFSEQFWFLL AIRLAFVILF EHVALCIKLI 430 440 450 460 470 480 AAWFVPDIPQ SVKNKVLEVK YQRLREKMWH GRQRLGGVGA GSRPPMPAHP TPASIFSARS TDV
	Isoform 3. Checksum: E197A8A4E397262F	FASTA	186	21,096
	10 20 30 40 50 60 MQGEESLRIL VEPEGDSFPL MEISTCETEA SEQWDYVLVA QRHTQRDPRQ ARQQQFLEEL 70 80 90 100 110 120 RRKGFHIKVI RDQKQVFFGI RADNSVFGLY RTFLLEPEGP APHAELAAPT TIPVTTSLRI 130 140 150 160 170 180 RIVNFVVMNN KTSAGETFED LMKDGVFEAR FPLHKVRGGP AWRGPWGGTL GWGLSLSVTR ARGRDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of long cDNA clones from human adult spleen. II. The complete sequences of 81 cDNA clones." Jikuya H., Takano J., Kikuno R., Hirosawa M., Nagase T., Nomura N., Ohara O. DNA Res. 10:49-57(2003) [PubMed: 12693554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS VAL-391 AND ARG-399. Tissue: Spleen.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), VARIANTS LEU-93; VAL-391 AND ARG-399. Tissue: Salivary gland and Thymus.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS LEU-93; VAL-391 AND ARG-399.
[5]	"TMEM16A confers receptor-activated calcium-dependent chloride conductance." Yang Y.D., Cho H., Koo J.Y., Tak M.H., Cho Y., Shim W.-S., Park S.P., Lee J., Lee B., Kim B.-M., Raouf R., Shin Y.K., Oh U. Nature 455:1210-1215(2008) [PubMed: 18724360] [Abstract] Cited for: IDENTIFICATION AS PUTATIVE CHLORIDE CHANNEL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK074088 mRNA. Translation: BAB84914.1. Sequence problems. AK096874 mRNA. Translation: BAG53386.1. AK303642 mRNA. Translation: BAG64646.1. AC138230 Genomic DNA. No translation available. BC128557 mRNA. Translation: AAI28558.1.
IPI	IPI00152780. IPI00922125. IPI00922523.
RefSeq	NP_001012302.2.
UniGene	Hs.501622
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000185101. Homo sapiens. [Contig view]
GeneID	338440.
KEGG	hsa:338440.
Organism-specific databases
GeneCards	GC11M000409.
HGNC	HGNC:20679. ANO9.
PharmGKB	PA134898400.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	A1A5B4.
OMA	A1A5B4. KCRSLRA.
Gene expression databases
Bgee	A1A5B4.
CleanEx	HS_ANO9.
Family and domain databases
InterPro	IPR007632. DUF590. [Graphical view]
PANTHER	PTHR12308. DUF590. 1 hit.
Pfam	PF04547. DUF590. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	97010.

Entry information

Entry name

ANO9_HUMAN

Accession

Primary (citable) accession number: A1A5B4
Secondary accession number(s): B3KUC4, B4E134, Q8TEN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 3, 2009
Last modified:	June 16, 2009
This is version 22 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)