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A1A546 (ISX_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Intestine-specific homeobox
Gene names
Name:Isx
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCMO1 expression in the intestine. Ref.3 Ref.4

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed in intestinal epithelial cells from the duodenum to the proximal colon. Ref.3 Ref.4

Developmental stage

Appears late in development, just before the villus transition in intestine morphogenesis. Restricted to the epithelial compartment in both adult and fetal intestine. Ref.3

Disruption phenotype

Mice appear healthy for at least 1 year and display normal histological features in the gut. They however show defects in intestinal gene expression with a dysregulation of the scavenger receptor Scarb1. Ref.3

Sequence similarities

Contains 1 homeobox DNA-binding domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A1A546-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A1A546-2)

The sequence of this isoform differs from the canonical sequence as follows:
     150-240: EAGLALPSNM...KWGSICATST → SCADTHCYDY...LSKQATLLSQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Intestine-specific homeobox
PRO_0000288603

Regions

DNA binding78 – 13760Homeobox

Natural variations

Alternative sequence150 – 24091EAGLA…CATST → SCADTHCYDYIGTSHRMLPT LSDSAPFKLVPYTDSPCPMA PMGPTAPAWPSHPASLCPYL HVPTPTPQVGQHLCNFNIGT DFSLSKQATLLSQ in isoform 2.
VSP_025717

Experimental info

Sequence conflict1761P → L in BAB31309. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 6, 2007. Version 2.
Checksum: C7BFA37A5950FA1B

FASTA24027,169
        10         20         30         40         50         60 
MAGPTIHRDM EKSSGYCEAP ENLGLSFSIE AILKKPTERR SLPRPQSICK EDSRQTTIPG 

        70         80         90        100        110        120 
SKLERPPQDQ PQEEKKNKRR VRTTFTTEQL QELEKLFHFT HYPDIHVRSQ LASRINLPEA 

       130        140        150        160        170        180 
RVQIWFQNQR AKWRKQEKSG NLSAPQQPGE AGLALPSNMD VSGPVLTPTA MTTLVPPTEC 

       190        200        210        220        230        240 
CLLSQTQLPS SWFPTQIPLV PWHPWDLQPL PGPLTQHPCV PTFMFPPLHP KWGSICATST 

« Hide

Isoform 2 [UniParc].

Checksum: 94AB50ADCACC7EF3
Show »

FASTA24227,344

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Cecum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]"A dynamic expression survey identifies transcription factors relevant in mouse digestive tract development."
Choi M.Y., Romer A.I., Hu M., Lepourcelet M., Mechoor A., Yesilaltay A., Krieger M., Gray P.A., Shivdasani R.A.
Development 133:4119-4129(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[4]"Isx participates in the maintenance of vitamin A metabolism by regulation of beta-carotene 15,15'-monooxygenase (Bcmo1) expression."
Seino Y., Miki T., Kiyonari H., Abe T., Fujimoto W., Kimura K., Takeuchi A., Takahashi Y., Oiso Y., Iwanaga T., Seino S.
J. Biol. Chem. 283:4905-4911(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK018615 mRNA. Translation: BAB31309.1.
BC128289 mRNA. Translation: AAI28290.2.
BC128290 mRNA. Translation: AAI28291.2.
RefSeqNP_082113.2. NM_027837.3.
XP_006531433.1. XM_006531370.1.
UniGeneMm.158735.

3D structure databases

ProteinModelPortalA1A546.
SMRA1A546. Positions 79-138.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000034034.

PTM databases

PhosphoSiteA1A546.

Proteomic databases

PRIDEA1A546.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034034; ENSMUSP00000034034; ENSMUSG00000031621. [A1A546-1]
ENSMUST00000174427; ENSMUSP00000134368; ENSMUSG00000031621. [A1A546-2]
GeneID71597.
KEGGmmu:71597.
UCSCuc009mgv.1. mouse. [A1A546-1]
uc012ggb.1. mouse. [A1A546-2]

Organism-specific databases

CTD91464.
MGIMGI:1918847. Isx.

Phylogenomic databases

eggNOGNOG252360.
GeneTreeENSGT00740000114982.
HOGENOMHOG000052524.
HOVERGENHBG096596.
InParanoidA1A546.
OMAGYCEAPE.
OrthoDBEOG7MSMQF.
TreeFamTF315976.

Gene expression databases

ArrayExpressA1A546.
BgeeA1A546.
CleanExMM_ISX.
GenevestigatorA1A546.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio334069.
PROA1A546.
SOURCESearch...

Entry information

Entry nameISX_MOUSE
AccessionPrimary (citable) accession number: A1A546
Secondary accession number(s): A1A545, Q9D2Z0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: March 19, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot