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Protein

Intestine-specific homeobox

Gene

Isx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCO1 expression in the intestine.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi78 – 137HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • sequence-specific DNA binding Source: MGI
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI

GO - Biological processi

  • negative regulation of intestinal absorption Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • regulation of vitamin A metabolic process Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Intestine-specific homeobox
Gene namesi
Name:Isx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1918847 Isx

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice appear healthy for at least 1 year and display normal histological features in the gut. They however show defects in intestinal gene expression with a dysregulation of the scavenger receptor Scarb1.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002886031 – 240Intestine-specific homeoboxAdd BLAST240

Proteomic databases

PaxDbiA1A546
PRIDEiA1A546

PTM databases

iPTMnetiA1A546
PhosphoSitePlusiA1A546

Expressioni

Tissue specificityi

Expressed in intestinal epithelial cells from the duodenum to the proximal colon.2 Publications

Developmental stagei

Appears late in development, just before the villus transition in intestine morphogenesis. Restricted to the epithelial compartment in both adult and fetal intestine.1 Publication

Gene expression databases

BgeeiENSMUSG00000031621
CleanExiMM_ISX
ExpressionAtlasiA1A546 baseline and differential
GenevisibleiA1A546 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034034

Structurei

3D structure databases

ProteinModelPortaliA1A546
SMRiA1A546
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490 Eukaryota
ENOG410YIJ3 LUCA
GeneTreeiENSGT00900000140809
HOGENOMiHOG000052524
HOVERGENiHBG096596
InParanoidiA1A546
OMAiGYCEAPE
OrthoDBiEOG091G0NQL
PhylomeDBiA1A546
TreeFamiTF315976

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A1A546-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPTIHRDM EKSSGYCEAP ENLGLSFSIE AILKKPTERR SLPRPQSICK
60 70 80 90 100
EDSRQTTIPG SKLERPPQDQ PQEEKKNKRR VRTTFTTEQL QELEKLFHFT
110 120 130 140 150
HYPDIHVRSQ LASRINLPEA RVQIWFQNQR AKWRKQEKSG NLSAPQQPGE
160 170 180 190 200
AGLALPSNMD VSGPVLTPTA MTTLVPPTEC CLLSQTQLPS SWFPTQIPLV
210 220 230 240
PWHPWDLQPL PGPLTQHPCV PTFMFPPLHP KWGSICATST
Length:240
Mass (Da):27,169
Last modified:February 6, 2007 - v2
Checksum:iC7BFA37A5950FA1B
GO
Isoform 2 (identifier: A1A546-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     150-240: EAGLALPSNM...KWGSICATST → SCADTHCYDY...LSKQATLLSQ

Note: No experimental confirmation available.
Show »
Length:242
Mass (Da):27,344
Checksum:i94AB50ADCACC7EF3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176P → L in BAB31309 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025717150 – 240EAGLA…CATST → SCADTHCYDYIGTSHRMLPT LSDSAPFKLVPYTDSPCPMA PMGPTAPAWPSHPASLCPYL HVPTPTPQVGQHLCNFNIGT DFSLSKQATLLSQ in isoform 2. 1 PublicationAdd BLAST91

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018615 mRNA Translation: BAB31309.1
BC128289 mRNA Translation: AAI28290.2
BC128290 mRNA Translation: AAI28291.2
CCDSiCCDS40391.1 [A1A546-1]
CCDS80899.1 [A1A546-2]
RefSeqiNP_001281207.1, NM_001294278.1 [A1A546-2]
NP_082113.2, NM_027837.3 [A1A546-1]
UniGeneiMm.158735

Genome annotation databases

EnsembliENSMUST00000034034; ENSMUSP00000034034; ENSMUSG00000031621 [A1A546-1]
ENSMUST00000174427; ENSMUSP00000134368; ENSMUSG00000031621 [A1A546-2]
GeneIDi71597
KEGGimmu:71597
UCSCiuc009mgv.1 mouse [A1A546-1]
uc012ggb.1 mouse [A1A546-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiISX_MOUSE
AccessioniPrimary (citable) accession number: A1A546
Secondary accession number(s): A1A545, Q9D2Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: February 6, 2007
Last modified: March 28, 2018
This is version 94 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health