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A1A4S6 (RHG10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rho GTPase-activating protein 10
Alternative name(s):
GTPase regulator associated with focal adhesion kinase 2
Graf-related protein 2
Rho-type GTPase-activating protein 10
Gene names
Name:ARHGAP10
Synonyms:GRAF2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length786 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death By similarity. Ref.1

Subunit structure

Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity By similarity. Ref.1

Subcellular location

Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Cell membrane By similarity. Note: Association to cell membrane is dependent on PH domain By similarity.

Tissue specificity

High levels of expression in heart and skeletal muscle. Ref.1

Post-translational modification

Phosphorylated. Phosphorylated in vitro by constitutive active PKN3. Ref.1

Sequence similarities

Contains 1 BAR domain.

Contains 1 PH domain.

Contains 1 Rho-GAP domain.

Contains 1 SH3 domain.

Sequence caution

The sequence AAI26900.1 differs from that shown. Reason: Frameshift at position 785.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PKN3Q6P5Z24EBI-1390944,EBI-1384335

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 786786Rho GTPase-activating protein 10
PRO_0000304914

Regions

Domain7 – 262256BAR
Domain265 – 372108PH
Domain389 – 574186Rho-GAP
Domain728 – 78659SH3

Natural variations

Natural variant4881P → S.
Corresponds to variant rs17024215 [ dbSNP | Ensembl ].
VAR_049141
Natural variant6841M → V. Ref.1
Corresponds to variant rs2276932 [ dbSNP | Ensembl ].
VAR_035114

Experimental info

Sequence conflict7121F → S in AAI09030. Ref.2
Sequence conflict7501E → D in BAB61771. Ref.1

Secondary structure

............... 786
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
A1A4S6 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 7D7006CEAC0094BD

FASTA78689,375
        10         20         30         40         50         60 
MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT KSLSVAQRKF 

        70         80         90        100        110        120 
AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ REIMALSVTE TLIKPLEKFR 

       130        140        150        160        170        180 
KEQLGAVKEE KKKFDKETEK NYSLIDKHLN LSAKKKDSHL QEADIQVEQN RQHFYELSLE 

       190        200        210        220        230        240 
YVCKLQEIQE RKKFEFVEPM LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE 

       250        260        270        280        290        300 
GTRSEVEELM NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF 

       310        320        330        340        350        360 
NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL TMQAFSEEER 

       370        380        390        400        410        420 
KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR KCISAVETRG INDQGLYRVV 

       430        440        450        460        470        480 
GVSSKVQRLL SMLMDVKTCN EVDLENSADW EVKTITSALK QYLRSLPEPL MTYELHGDFI 

       490        500        510        520        530        540 
VPAKSGSPES RVNAIHFLVH KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP 

       550        560        570        580        590        600 
TLMRPQEETV AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS 

       610        620        630        640        650        660 
KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA VPGPPGPDKN 

       670        680        690        700        710        720 
HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS AVTPLSPGSS PFPFSPPATV 

       730        740        750        760        770        780 
ADKPPESIRS RKARAVYPCE AEHSSELSFE IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ 


NYVKLL 

« Hide

References

« Hide 'large scale' references
[1]"PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family."
Shibata H., Oishi K., Yamagiwa A., Matsumoto M., Mukai H., Ono Y.
J. Biochem. 130:23-31(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PKN3, TISSUE SPECIFICITY, PHOSPHORYLATION, VARIANT VAL-684.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]"Solution NMR structure of SH3 domain 1 of Rho GTPase-activating protein 10 from Homo sapiens, Northeast structural genomics consortium (NESG) target HR9129A."
Northeast structural genomics consortium (NESG)
Submitted (JAN-2014) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 728-786.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB050785 mRNA. Translation: BAB61771.1.
BC011920 mRNA. Translation: AAH11920.2.
BC109029 mRNA. Translation: AAI09030.1.
BC109030 mRNA. Translation: AAI09031.1.
BC126899 mRNA. Translation: AAI26900.1. Frameshift.
BC128055 mRNA. Translation: AAI28056.1.
RefSeqNP_078881.3. NM_024605.3.
UniGeneHs.368631.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2MIONMR-A728-786[»]
ProteinModelPortalA1A4S6.
SMRA1A4S6. Positions 2-369, 389-579, 731-786.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid122784. 5 interactions.
IntActA1A4S6. 1 interaction.
MINTMINT-203211.
STRING9606.ENSP00000336923.

PTM databases

PhosphoSiteA1A4S6.

Proteomic databases

PaxDbA1A4S6.
PRIDEA1A4S6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000336498; ENSP00000336923; ENSG00000071205.
GeneID79658.
KEGGhsa:79658.
UCSCuc003ilf.3. human.

Organism-specific databases

CTD79658.
GeneCardsGC04P148653.
HGNCHGNC:26099. ARHGAP10.
HPAHPA039589.
MIM609746. gene.
neXtProtNX_A1A4S6.
PharmGKBPA134904544.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG311191.
HOGENOMHOG000018767.
HOVERGENHBG067993.
InParanoidA1A4S6.
KOK13736.
OMACFDVEAA.
OrthoDBEOG71VSS5.
PhylomeDBA1A4S6.
TreeFamTF316851.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_578. Apoptosis.

Gene expression databases

ArrayExpressA1A4S6.
BgeeA1A4S6.
CleanExHS_ARHGAP10.
GenevestigatorA1A4S6.

Family and domain databases

Gene3D1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARHGAP10. human.
GenomeRNAi79658.
NextBio68834.
PROA1A4S6.
SOURCESearch...

Entry information

Entry nameRHG10_HUMAN
AccessionPrimary (citable) accession number: A1A4S6
Secondary accession number(s): A1L0S5 expand/collapse secondary AC list , Q2VPC4, Q2VPC5, Q96EV3, Q96S75
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM