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Protein

Rho GTPase-activating protein 10

Gene

ARHGAP10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle
R-HSA-211728 Regulation of PAK-2p34 activity by PS-GAP/RHG10

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 10
Alternative name(s):
GTPase regulator associated with focal adhesion kinase 2
Graf-related protein 2
Rho-type GTPase-activating protein 10
Gene namesi
Name:ARHGAP10
Synonyms:GRAF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000071205.11
HGNCiHGNC:26099 ARHGAP10
MIMi609746 gene
neXtProtiNX_A1A4S6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi79658
OpenTargetsiENSG00000071205
PharmGKBiPA134904544

Polymorphism and mutation databases

BioMutaiARHGAP10

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003049141 – 786Rho GTPase-activating protein 10Add BLAST786

Post-translational modificationi

Phosphorylated. Phosphorylated in vitro by constitutive active PKN3.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiA1A4S6
MaxQBiA1A4S6
PaxDbiA1A4S6
PRIDEiA1A4S6

PTM databases

iPTMnetiA1A4S6
PhosphoSitePlusiA1A4S6

Expressioni

Tissue specificityi

High levels of expression in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000071205
CleanExiHS_ARHGAP10
ExpressionAtlasiA1A4S6 baseline and differential
GenevisibleiA1A4S6 HS

Organism-specific databases

HPAiHPA039589

Interactioni

Subunit structurei

Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PKN3Q6P5Z24EBI-1390944,EBI-1384335

Protein-protein interaction databases

BioGridi122784, 11 interactors
CORUMiA1A4S6
IntActiA1A4S6, 6 interactors
MINTiA1A4S6
STRINGi9606.ENSP00000336923

Structurei

Secondary structure

1786
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi728 – 730Combined sources3
Beta strandi732 – 737Combined sources6
Beta strandi743 – 746Combined sources4
Beta strandi754 – 760Combined sources7
Beta strandi766 – 771Combined sources6
Beta strandi774 – 779Combined sources6
Helixi780 – 782Combined sources3
Beta strandi783 – 785Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MIONMR-A728-786[»]
ProteinModelPortaliA1A4S6
SMRiA1A4S6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 262BARAdd BLAST256
Domaini265 – 372PHPROSITE-ProRule annotationAdd BLAST108
Domaini389 – 574Rho-GAPPROSITE-ProRule annotationAdd BLAST186
Domaini728 – 786SH3PROSITE-ProRule annotationAdd BLAST59

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG1451 Eukaryota
ENOG410YJPS LUCA
GeneTreeiENSGT00890000139322
HOGENOMiHOG000018767
HOVERGENiHBG067993
InParanoidiA1A4S6
KOiK13736
OMAiCFDVEAA
OrthoDBiEOG091G01TT
PhylomeDBiA1A4S6
TreeFamiTF316851

Family and domain databases

CDDicd12065 SH3_GRAF2, 1 hit
Gene3Di1.10.555.10, 1 hit
1.20.1270.60, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR035486 GRAF2
IPR035485 GRAF2_SH3
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR12552:SF5 PTHR12552:SF5, 1 hit
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00620 RhoGAP, 1 hit
PF14604 SH3_9, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00324 RhoGAP, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48350 SSF48350, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS50238 RHOGAP, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

A1A4S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT
60 70 80 90 100
KSLSVAQRKF AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ
110 120 130 140 150
REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLN
160 170 180 190 200
LSAKKKDSHL QEADIQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM
210 220 230 240 250
LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM
260 270 280 290 300
NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF
310 320 330 340 350
NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL
360 370 380 390 400
TMQAFSEEER KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR
410 420 430 440 450
KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKTCN EVDLENSADW
460 470 480 490 500
EVKTITSALK QYLRSLPEPL MTYELHGDFI VPAKSGSPES RVNAIHFLVH
510 520 530 540 550
KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP TLMRPQEETV
560 570 580 590 600
AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS
610 620 630 640 650
KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA
660 670 680 690 700
VPGPPGPDKN HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS
710 720 730 740 750
AVTPLSPGSS PFPFSPPATV ADKPPESIRS RKARAVYPCE AEHSSELSFE
760 770 780
IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL
Length:786
Mass (Da):89,375
Last modified:January 23, 2007 - v1
Checksum:i7D7006CEAC0094BD
GO

Sequence cautioni

The sequence AAI26900 differs from that shown. Reason: Frameshift at position 785.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti712F → S in AAI09030 (PubMed:15489334).Curated1
Sequence conflicti750E → D in BAB61771 (PubMed:11432776).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049141488P → S. Corresponds to variant dbSNP:rs17024215Ensembl.1
Natural variantiVAR_035114684M → V1 PublicationCorresponds to variant dbSNP:rs2276932Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050785 mRNA Translation: BAB61771.1
BC011920 mRNA Translation: AAH11920.2
BC109029 mRNA Translation: AAI09030.1
BC109030 mRNA Translation: AAI09031.1
BC126899 mRNA Translation: AAI26900.1 Frameshift.
BC128055 mRNA Translation: AAI28056.1
CCDSiCCDS34075.1
RefSeqiNP_078881.3, NM_024605.3
UniGeneiHs.368631

Genome annotation databases

EnsembliENST00000336498; ENSP00000336923; ENSG00000071205
GeneIDi79658
KEGGihsa:79658
UCSCiuc003ilf.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRHG10_HUMAN
AccessioniPrimary (citable) accession number: A1A4S6
Secondary accession number(s): A1L0S5
, Q2VPC4, Q2VPC5, Q96EV3, Q96S75
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health