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A1A4S6

- RHG10_HUMAN

UniProt

A1A4S6 - RHG10_HUMAN

Protein

Rho GTPase-activating protein 10

Gene

ARHGAP10

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. Rho GTPase activator activity Source: Ensembl

    GO - Biological processi

    1. apoptotic process Source: Reactome
    2. cytoskeleton organization Source: Ensembl
    3. negative regulation of apoptotic process Source: Ensembl
    4. regulation of apoptotic process Source: Reactome
    5. regulation of small GTPase mediated signal transduction Source: Reactome
    6. small GTPase mediated signal transduction Source: Reactome

    Keywords - Molecular functioni

    GTPase activation

    Enzyme and pathway databases

    ReactomeiREACT_11051. Rho GTPase cycle.
    REACT_13528. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho GTPase-activating protein 10
    Alternative name(s):
    GTPase regulator associated with focal adhesion kinase 2
    Graf-related protein 2
    Rho-type GTPase-activating protein 10
    Gene namesi
    Name:ARHGAP10
    Synonyms:GRAF2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:26099. ARHGAP10.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Cell membrane By similarity
    Note: Association to cell membrane is dependent on PH domain.By similarity

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134904544.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 786786Rho GTPase-activating protein 10PRO_0000304914Add
    BLAST

    Post-translational modificationi

    Phosphorylated. Phosphorylated in vitro by constitutive active PKN3.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiA1A4S6.
    PaxDbiA1A4S6.
    PRIDEiA1A4S6.

    PTM databases

    PhosphoSiteiA1A4S6.

    Expressioni

    Tissue specificityi

    High levels of expression in heart and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiA1A4S6.
    BgeeiA1A4S6.
    CleanExiHS_ARHGAP10.
    GenevestigatoriA1A4S6.

    Organism-specific databases

    HPAiHPA039589.

    Interactioni

    Subunit structurei

    Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PKN3Q6P5Z24EBI-1390944,EBI-1384335

    Protein-protein interaction databases

    BioGridi122784. 5 interactions.
    IntActiA1A4S6. 1 interaction.
    MINTiMINT-203211.
    STRINGi9606.ENSP00000336923.

    Structurei

    Secondary structure

    1
    786
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi728 – 7303
    Beta strandi732 – 7376
    Beta strandi743 – 7464
    Beta strandi754 – 7607
    Beta strandi766 – 7716
    Beta strandi774 – 7796
    Helixi780 – 7823
    Beta strandi783 – 7853

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2MIONMR-A728-786[»]
    ProteinModelPortaliA1A4S6.
    SMRiA1A4S6. Positions 2-364, 389-579, 728-786.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 262256BARAdd
    BLAST
    Domaini265 – 372108PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini389 – 574186Rho-GAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini728 – 78659SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BAR domain.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG311191.
    HOGENOMiHOG000018767.
    HOVERGENiHBG067993.
    InParanoidiA1A4S6.
    KOiK13736.
    OMAiCFDVEAA.
    OrthoDBiEOG71VSS5.
    PhylomeDBiA1A4S6.
    TreeFamiTF316851.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00620. RhoGAP. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1A4S6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT    50
    KSLSVAQRKF AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ 100
    REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLN 150
    LSAKKKDSHL QEADIQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM 200
    LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM 250
    NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF 300
    NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL 350
    TMQAFSEEER KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR 400
    KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKTCN EVDLENSADW 450
    EVKTITSALK QYLRSLPEPL MTYELHGDFI VPAKSGSPES RVNAIHFLVH 500
    KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP TLMRPQEETV 550
    AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS 600
    KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA 650
    VPGPPGPDKN HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS 700
    AVTPLSPGSS PFPFSPPATV ADKPPESIRS RKARAVYPCE AEHSSELSFE 750
    IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL 786
    Length:786
    Mass (Da):89,375
    Last modified:January 23, 2007 - v1
    Checksum:i7D7006CEAC0094BD
    GO

    Sequence cautioni

    The sequence AAI26900.1 differs from that shown. Reason: Frameshift at position 785.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti712 – 7121F → S in AAI09030. (PubMed:15489334)Curated
    Sequence conflicti750 – 7501E → D in BAB61771. (PubMed:11432776)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti488 – 4881P → S.
    Corresponds to variant rs17024215 [ dbSNP | Ensembl ].
    VAR_049141
    Natural varianti684 – 6841M → V.1 Publication
    Corresponds to variant rs2276932 [ dbSNP | Ensembl ].
    VAR_035114

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB050785 mRNA. Translation: BAB61771.1.
    BC011920 mRNA. Translation: AAH11920.2.
    BC109029 mRNA. Translation: AAI09030.1.
    BC109030 mRNA. Translation: AAI09031.1.
    BC126899 mRNA. Translation: AAI26900.1. Frameshift.
    BC128055 mRNA. Translation: AAI28056.1.
    CCDSiCCDS34075.1.
    RefSeqiNP_078881.3. NM_024605.3.
    UniGeneiHs.368631.

    Genome annotation databases

    EnsembliENST00000336498; ENSP00000336923; ENSG00000071205.
    GeneIDi79658.
    KEGGihsa:79658.
    UCSCiuc003ilf.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB050785 mRNA. Translation: BAB61771.1 .
    BC011920 mRNA. Translation: AAH11920.2 .
    BC109029 mRNA. Translation: AAI09030.1 .
    BC109030 mRNA. Translation: AAI09031.1 .
    BC126899 mRNA. Translation: AAI26900.1 . Frameshift.
    BC128055 mRNA. Translation: AAI28056.1 .
    CCDSi CCDS34075.1.
    RefSeqi NP_078881.3. NM_024605.3.
    UniGenei Hs.368631.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2MIO NMR - A 728-786 [» ]
    ProteinModelPortali A1A4S6.
    SMRi A1A4S6. Positions 2-364, 389-579, 728-786.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 122784. 5 interactions.
    IntActi A1A4S6. 1 interaction.
    MINTi MINT-203211.
    STRINGi 9606.ENSP00000336923.

    PTM databases

    PhosphoSitei A1A4S6.

    Proteomic databases

    MaxQBi A1A4S6.
    PaxDbi A1A4S6.
    PRIDEi A1A4S6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000336498 ; ENSP00000336923 ; ENSG00000071205 .
    GeneIDi 79658.
    KEGGi hsa:79658.
    UCSCi uc003ilf.3. human.

    Organism-specific databases

    CTDi 79658.
    GeneCardsi GC04P148653.
    HGNCi HGNC:26099. ARHGAP10.
    HPAi HPA039589.
    MIMi 609746. gene.
    neXtProti NX_A1A4S6.
    PharmGKBi PA134904544.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG311191.
    HOGENOMi HOG000018767.
    HOVERGENi HBG067993.
    InParanoidi A1A4S6.
    KOi K13736.
    OMAi CFDVEAA.
    OrthoDBi EOG71VSS5.
    PhylomeDBi A1A4S6.
    TreeFami TF316851.

    Enzyme and pathway databases

    Reactomei REACT_11051. Rho GTPase cycle.
    REACT_13528. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

    Miscellaneous databases

    ChiTaRSi ARHGAP10. human.
    GenomeRNAii 79658.
    NextBioi 68834.
    PROi A1A4S6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi A1A4S6.
    Bgeei A1A4S6.
    CleanExi HS_ARHGAP10.
    Genevestigatori A1A4S6.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00620. RhoGAP. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00324. RhoGAP. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF50044. SSF50044. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50238. RHOGAP. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family."
      Shibata H., Oishi K., Yamagiwa A., Matsumoto M., Mukai H., Ono Y.
      J. Biochem. 130:23-31(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PKN3, TISSUE SPECIFICITY, PHOSPHORYLATION, VARIANT VAL-684.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. "Solution NMR structure of SH3 domain 1 of Rho GTPase-activating protein 10 from Homo sapiens, Northeast structural genomics consortium (NESG) target HR9129A."
      Northeast structural genomics consortium (NESG)
      Submitted (JAN-2014) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 728-786.

    Entry informationi

    Entry nameiRHG10_HUMAN
    AccessioniPrimary (citable) accession number: A1A4S6
    Secondary accession number(s): A1L0S5
    , Q2VPC4, Q2VPC5, Q96EV3, Q96S75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3