A1A4S6 (RHG10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rho GTPase-activating protein 10

Alternative name(s):
GTPase regulator associated with focal adhesion kinase 2
Graf-related protein 2
Rho-type GTPase-activating protein 10

Gene names

Name:	ARHGAP10
Synonyms:	GRAF2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	786 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death By similarity. Ref.1
Subunit structure	Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity By similarity. Ref.1
Subcellular location	Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity. Cell membrane By similarity. Note: Association to cell membrane is dependent on PH domain By similarity.
Tissue specificity	High levels of expression in heart and skeletal muscle. Ref.1
Post-translational modification	Phosphorylated. Phosphorylated in vitro by constitutive active PKN3. Ref.1
Sequence similarities	Contains 1 BAR domain. Contains 1 PH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.
Sequence caution	The sequence AAI26900.1 differs from that shown. Reason: Frameshift at position 785.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	SH3 domain
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Traceable author statement. Source: Reactome cytoskeleton organization Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara regulation of apoptotic process Traceable author statement. Source: Reactome regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	Rho GTPase activator activity Inferred from electronic annotation. Source: Compara phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PKN3	Q6P5Z2	4	EBI-1390944,EBI-1384335

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 786	786	Rho GTPase-activating protein 10		PRO_0000304914
Regions
	Domain	7 – 262	256	BAR
	Domain	265 – 372	108	PH
	Domain	389 – 574	186	Rho-GAP
	Domain	728 – 786	59	SH3
Amino acid modifications
	Modified residue	109	1	Phosphothreonine By similarity
	Modified residue	111	1	Phosphothreonine By similarity
Natural variations
	Natural variant	488	1	P → S. Corresponds to variant rs17024215 [ dbSNP | Ensembl ].		VAR_049141
	Natural variant	684	1	M → V. Ref.1 Corresponds to variant rs2276932 [ dbSNP | Ensembl ].		VAR_035114
Experimental info
	Sequence conflict	712	1	F → S in AAI09030. Ref.2
	Sequence conflict	750	1	E → D in BAB61771. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A1A4S6 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 7D7006CEAC0094BD	FASTA	786	89,375
	10 20 30 40 50 60 MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT KSLSVAQRKF 70 80 90 100 110 120 AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ REIMALSVTE TLIKPLEKFR 130 140 150 160 170 180 KEQLGAVKEE KKKFDKETEK NYSLIDKHLN LSAKKKDSHL QEADIQVEQN RQHFYELSLE 190 200 210 220 230 240 YVCKLQEIQE RKKFEFVEPM LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE 250 260 270 280 290 300 GTRSEVEELM NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF 310 320 330 340 350 360 NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL TMQAFSEEER 370 380 390 400 410 420 KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR KCISAVETRG INDQGLYRVV 430 440 450 460 470 480 GVSSKVQRLL SMLMDVKTCN EVDLENSADW EVKTITSALK QYLRSLPEPL MTYELHGDFI 490 500 510 520 530 540 VPAKSGSPES RVNAIHFLVH KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP 550 560 570 580 590 600 TLMRPQEETV AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS 610 620 630 640 650 660 KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA VPGPPGPDKN 670 680 690 700 710 720 HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS AVTPLSPGSS PFPFSPPATV 730 740 750 760 770 780 ADKPPESIRS RKARAVYPCE AEHSSELSFE IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family." Shibata H., Oishi K., Yamagiwa A., Matsumoto M., Mukai H., Ono Y. J. Biochem. 130:23-31(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PKN3, TISSUE SPECIFICITY, PHOSPHORYLATION, VARIANT VAL-684.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB050785 mRNA. Translation: BAB61771.1. BC011920 mRNA. Translation: AAH11920.2. BC109029 mRNA. Translation: AAI09030.1. BC109030 mRNA. Translation: AAI09031.1. BC126899 mRNA. Translation: AAI26900.1. Frameshift. BC128055 mRNA. Translation: AAI28056.1.
IPI	IPI00290332.
RefSeq	NP_078881.3. NM_024605.3.
UniGene	Hs.368631.
3D structure databases
ProteinModelPortal	A1A4S6.
ModBase	Search...
Protein-protein interaction databases
IntAct	A1A4S6. 1 interaction.
MINT	MINT-203211.
STRING	9606.ENSP00000336923.
PTM databases
PhosphoSite	A1A4S6.
Proteomic databases
PaxDb	A1A4S6.
PRIDE	A1A4S6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000336498; ENSP00000336923; ENSG00000071205.
GeneID	79658.
KEGG	hsa:79658.
UCSC	uc003ilf.3. human.
Organism-specific databases
CTD	79658.
GeneCards	GC04P148653.
HGNC	HGNC:26099. ARHGAP10.
HPA	HPA039589.
MIM	609746. gene.
neXtProt	NX_A1A4S6.
PharmGKB	PA134904544.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG311191.
HOGENOM	HOG000018767.
HOVERGEN	HBG067993.
InParanoid	A1A4S6.
KO	K13736.
OMA	TSAMKQY.
OrthoDB	EOG49GKG8.
PhylomeDB	A1A4S6.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_578. Apoptosis.
Gene expression databases
ArrayExpress	A1A4S6.
Bgee	A1A4S6.
CleanEx	HS_ARHGAP10.
Genevestigator	A1A4S6.
Family and domain databases
Gene3D	1.10.555.10. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00620. RhoGAP. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00324. RhoGAP. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS51021. BAR. False negative. PS50003. PH_DOMAIN. 1 hit. PS50238. RHOGAP. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ARHGAP10. human.
GenomeRNAi	79658.
NextBio	68834.
SOURCE	Search...

Entry information

Entry name

RHG10_HUMAN

Accession

Primary (citable) accession number: A1A4S6
Secondary accession number(s): A1L0S5 , Q2VPC4, Q2VPC5, Q96EV3, Q96S75

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.