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Protein

Rho GTPase-activating protein 10

Gene

ARHGAP10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death (By similarity).By similarity

GO - Molecular functioni

  1. Rho GTPase activator activity Source: Ensembl

GO - Biological processi

  1. apoptotic process Source: Reactome
  2. cytoskeleton organization Source: Ensembl
  3. negative regulation of apoptotic process Source: Ensembl
  4. regulation of apoptotic process Source: Reactome
  5. regulation of small GTPase mediated signal transduction Source: Reactome
  6. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_13528. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 10
Alternative name(s):
GTPase regulator associated with focal adhesion kinase 2
Graf-related protein 2
Rho-type GTPase-activating protein 10
Gene namesi
Name:ARHGAP10
Synonyms:GRAF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:26099. ARHGAP10.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Cell membrane By similarity
Note: Association to cell membrane is dependent on PH domain.By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134904544.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Rho GTPase-activating protein 10PRO_0000304914Add
BLAST

Post-translational modificationi

Phosphorylated. Phosphorylated in vitro by constitutive active PKN3.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA1A4S6.
PaxDbiA1A4S6.
PRIDEiA1A4S6.

PTM databases

PhosphoSiteiA1A4S6.

Expressioni

Tissue specificityi

High levels of expression in heart and skeletal muscle.1 Publication

Gene expression databases

BgeeiA1A4S6.
CleanExiHS_ARHGAP10.
ExpressionAtlasiA1A4S6. baseline and differential.
GenevestigatoriA1A4S6.

Organism-specific databases

HPAiHPA039589.

Interactioni

Subunit structurei

Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PKN3Q6P5Z24EBI-1390944,EBI-1384335

Protein-protein interaction databases

BioGridi122784. 6 interactions.
IntActiA1A4S6. 1 interaction.
MINTiMINT-203211.
STRINGi9606.ENSP00000336923.

Structurei

Secondary structure

1
786
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi728 – 7303Combined sources
Beta strandi732 – 7376Combined sources
Beta strandi743 – 7464Combined sources
Beta strandi754 – 7607Combined sources
Beta strandi766 – 7716Combined sources
Beta strandi774 – 7796Combined sources
Helixi780 – 7823Combined sources
Beta strandi783 – 7853Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MIONMR-A728-786[»]
ProteinModelPortaliA1A4S6.
SMRiA1A4S6. Positions 2-364, 389-579, 728-786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 262256BARAdd
BLAST
Domaini265 – 372108PHPROSITE-ProRule annotationAdd
BLAST
Domaini389 – 574186Rho-GAPPROSITE-ProRule annotationAdd
BLAST
Domaini728 – 78659SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BAR domain.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG311191.
GeneTreeiENSGT00780000121833.
HOGENOMiHOG000018767.
HOVERGENiHBG067993.
InParanoidiA1A4S6.
KOiK13736.
OMAiCFDVEAA.
OrthoDBiEOG71VSS5.
PhylomeDBiA1A4S6.
TreeFamiTF316851.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1A4S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT
60 70 80 90 100
KSLSVAQRKF AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ
110 120 130 140 150
REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLN
160 170 180 190 200
LSAKKKDSHL QEADIQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM
210 220 230 240 250
LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM
260 270 280 290 300
NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF
310 320 330 340 350
NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL
360 370 380 390 400
TMQAFSEEER KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR
410 420 430 440 450
KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKTCN EVDLENSADW
460 470 480 490 500
EVKTITSALK QYLRSLPEPL MTYELHGDFI VPAKSGSPES RVNAIHFLVH
510 520 530 540 550
KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP TLMRPQEETV
560 570 580 590 600
AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS
610 620 630 640 650
KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA
660 670 680 690 700
VPGPPGPDKN HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS
710 720 730 740 750
AVTPLSPGSS PFPFSPPATV ADKPPESIRS RKARAVYPCE AEHSSELSFE
760 770 780
IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL
Length:786
Mass (Da):89,375
Last modified:January 22, 2007 - v1
Checksum:i7D7006CEAC0094BD
GO

Sequence cautioni

The sequence AAI26900.1 differs from that shown. Reason: Frameshift at position 785. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti712 – 7121F → S in AAI09030 (PubMed:15489334).Curated
Sequence conflicti750 – 7501E → D in BAB61771 (PubMed:11432776).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti488 – 4881P → S.
Corresponds to variant rs17024215 [ dbSNP | Ensembl ].
VAR_049141
Natural varianti684 – 6841M → V.1 Publication
Corresponds to variant rs2276932 [ dbSNP | Ensembl ].
VAR_035114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050785 mRNA. Translation: BAB61771.1.
BC011920 mRNA. Translation: AAH11920.2.
BC109029 mRNA. Translation: AAI09030.1.
BC109030 mRNA. Translation: AAI09031.1.
BC126899 mRNA. Translation: AAI26900.1. Frameshift.
BC128055 mRNA. Translation: AAI28056.1.
CCDSiCCDS34075.1.
RefSeqiNP_078881.3. NM_024605.3.
UniGeneiHs.368631.

Genome annotation databases

EnsembliENST00000336498; ENSP00000336923; ENSG00000071205.
GeneIDi79658.
KEGGihsa:79658.
UCSCiuc003ilf.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB050785 mRNA. Translation: BAB61771.1.
BC011920 mRNA. Translation: AAH11920.2.
BC109029 mRNA. Translation: AAI09030.1.
BC109030 mRNA. Translation: AAI09031.1.
BC126899 mRNA. Translation: AAI26900.1. Frameshift.
BC128055 mRNA. Translation: AAI28056.1.
CCDSiCCDS34075.1.
RefSeqiNP_078881.3. NM_024605.3.
UniGeneiHs.368631.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MIONMR-A728-786[»]
ProteinModelPortaliA1A4S6.
SMRiA1A4S6. Positions 2-364, 389-579, 728-786.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122784. 6 interactions.
IntActiA1A4S6. 1 interaction.
MINTiMINT-203211.
STRINGi9606.ENSP00000336923.

PTM databases

PhosphoSiteiA1A4S6.

Proteomic databases

MaxQBiA1A4S6.
PaxDbiA1A4S6.
PRIDEiA1A4S6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000336498; ENSP00000336923; ENSG00000071205.
GeneIDi79658.
KEGGihsa:79658.
UCSCiuc003ilf.3. human.

Organism-specific databases

CTDi79658.
GeneCardsiGC04P148653.
HGNCiHGNC:26099. ARHGAP10.
HPAiHPA039589.
MIMi609746. gene.
neXtProtiNX_A1A4S6.
PharmGKBiPA134904544.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG311191.
GeneTreeiENSGT00780000121833.
HOGENOMiHOG000018767.
HOVERGENiHBG067993.
InParanoidiA1A4S6.
KOiK13736.
OMAiCFDVEAA.
OrthoDBiEOG71VSS5.
PhylomeDBiA1A4S6.
TreeFamiTF316851.

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_13528. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Miscellaneous databases

ChiTaRSiARHGAP10. human.
GenomeRNAii79658.
NextBioi68834.
PROiA1A4S6.
SOURCEiSearch...

Gene expression databases

BgeeiA1A4S6.
CleanExiHS_ARHGAP10.
ExpressionAtlasiA1A4S6. baseline and differential.
GenevestigatoriA1A4S6.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family."
    Shibata H., Oishi K., Yamagiwa A., Matsumoto M., Mukai H., Ono Y.
    J. Biochem. 130:23-31(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PKN3, TISSUE SPECIFICITY, PHOSPHORYLATION, VARIANT VAL-684.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Solution NMR structure of SH3 domain 1 of Rho GTPase-activating protein 10 from Homo sapiens, Northeast structural genomics consortium (NESG) target HR9129A."
    Northeast structural genomics consortium (NESG)
    Submitted (DEC-2013) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 728-786.

Entry informationi

Entry nameiRHG10_HUMAN
AccessioniPrimary (citable) accession number: A1A4S6
Secondary accession number(s): A1L0S5
, Q2VPC4, Q2VPC5, Q96EV3, Q96S75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2007
Last sequence update: January 22, 2007
Last modified: February 3, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.