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A1A4S6

- RHG10_HUMAN

UniProt

A1A4S6 - RHG10_HUMAN

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Protein
Rho GTPase-activating protein 10
Gene
ARHGAP10, GRAF2
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death By similarity.1 Publication

GO - Molecular functioni

  1. Rho GTPase activator activity Source: Ensembl
  2. protein binding Source: IntAct

GO - Biological processi

  1. apoptotic process Source: Reactome
  2. cytoskeleton organization Source: Ensembl
  3. negative regulation of apoptotic process Source: Ensembl
  4. regulation of apoptotic process Source: Reactome
  5. regulation of small GTPase mediated signal transduction Source: Reactome
  6. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_13528. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 10
Alternative name(s):
GTPase regulator associated with focal adhesion kinase 2
Graf-related protein 2
Rho-type GTPase-activating protein 10
Gene namesi
Synonyms:GRAF2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 4

Organism-specific databases

HGNCiHGNC:26099. ARHGAP10.

Subcellular locationi

Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Cell membrane By similarity
Note: Association to cell membrane is dependent on PH domain By similarity.

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134904544.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Rho GTPase-activating protein 10
PRO_0000304914Add
BLAST

Post-translational modificationi

Phosphorylated. Phosphorylated in vitro by constitutive active PKN3.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA1A4S6.
PaxDbiA1A4S6.
PRIDEiA1A4S6.

PTM databases

PhosphoSiteiA1A4S6.

Expressioni

Tissue specificityi

High levels of expression in heart and skeletal muscle.1 Publication

Gene expression databases

ArrayExpressiA1A4S6.
BgeeiA1A4S6.
CleanExiHS_ARHGAP10.
GenevestigatoriA1A4S6.

Organism-specific databases

HPAiHPA039589.

Interactioni

Subunit structurei

Interacts with PKN3. Interacts with caspase-activated PAK2 proteolytic fragment PAK-2p34; the interaction does not affect ARHGAP10 GTPase activation activity towards RHOA and CDC42. Interacts via its SH3 domain with PTK2/FAK1. Interacts with PTK2B/PYK2; the interaction negatively regulates ARHGAP10 GTPase-activating activity By similarity.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PKN3Q6P5Z24EBI-1390944,EBI-1384335

Protein-protein interaction databases

BioGridi122784. 5 interactions.
IntActiA1A4S6. 1 interaction.
MINTiMINT-203211.
STRINGi9606.ENSP00000336923.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi728 – 7303
Beta strandi732 – 7376
Beta strandi743 – 7464
Beta strandi754 – 7607
Beta strandi766 – 7716
Beta strandi774 – 7796
Helixi780 – 7823
Beta strandi783 – 7853

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MIONMR-A728-786[»]
ProteinModelPortaliA1A4S6.
SMRiA1A4S6. Positions 2-364, 389-579, 728-786.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 262256BAR
Add
BLAST
Domaini265 – 372108PH
Add
BLAST
Domaini389 – 574186Rho-GAP
Add
BLAST
Domaini728 – 78659SH3
Add
BLAST

Sequence similaritiesi

Contains 1 BAR domain.
Contains 1 PH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiNOG311191.
HOGENOMiHOG000018767.
HOVERGENiHBG067993.
InParanoidiA1A4S6.
KOiK13736.
OMAiCFDVEAA.
OrthoDBiEOG71VSS5.
PhylomeDBiA1A4S6.
TreeFamiTF316851.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1A4S6-1 [UniParc]FASTAAdd to Basket

« Hide

MGLQPLEFSD CYLDSPWFRE RIRAHEAELE RTNKFIKELI KDGKNLIAAT    50
KSLSVAQRKF AHSLRDFKFE FIGDAVTDDE RCIDASLREF SNFLKNLEEQ 100
REIMALSVTE TLIKPLEKFR KEQLGAVKEE KKKFDKETEK NYSLIDKHLN 150
LSAKKKDSHL QEADIQVEQN RQHFYELSLE YVCKLQEIQE RKKFEFVEPM 200
LSFFQGMFTF YHQGHELAKD FNHYKMELQI NIQNTRNRFE GTRSEVEELM 250
NKIRQNPKDH KRASQFTAEG YLYVQEKRPA PFGSSWVKHY CMYRKAAKKF 300
NMIPFEHRSG GKLGDGEVFF LKECTKRHTD SIDRRFCFDI EAADRPGVSL 350
TMQAFSEEER KQWLEALGGK EALSHSFNTA IIPRPEGNAQ LDKMGFTIIR 400
KCISAVETRG INDQGLYRVV GVSSKVQRLL SMLMDVKTCN EVDLENSADW 450
EVKTITSALK QYLRSLPEPL MTYELHGDFI VPAKSGSPES RVNAIHFLVH 500
KLPEKNKEML DILVKHLTNV SNHSKQNLMT VANLGVVFGP TLMRPQEETV 550
AALMDLKFQN IVVEILIENH EKIFRTPPDT TFPEPTCLSA SPPNAPPRQS 600
KRQGQRTKRP VAVYNLCLEL EDGDNPYPSK EDTPTSSLDS LSSPSPVTTA 650
VPGPPGPDKN HLLADGGSFG DWASTIPGQT RSSMVQWLNP QSPTTTSSNS 700
AVTPLSPGSS PFPFSPPATV ADKPPESIRS RKARAVYPCE AEHSSELSFE 750
IGAIFEDVQT SREPGWLEGT LNGKRGLIPQ NYVKLL 786
Length:786
Mass (Da):89,375
Last modified:January 23, 2007 - v1
Checksum:i7D7006CEAC0094BD
GO

Sequence cautioni

The sequence AAI26900.1 differs from that shown. Reason: Frameshift at position 785.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti488 – 4881P → S.
Corresponds to variant rs17024215 [ dbSNP | Ensembl ].
VAR_049141
Natural varianti684 – 6841M → V.1 Publication
Corresponds to variant rs2276932 [ dbSNP | Ensembl ].
VAR_035114

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti712 – 7121F → S in AAI09030. 1 Publication
Sequence conflicti750 – 7501E → D in BAB61771. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB050785 mRNA. Translation: BAB61771.1.
BC011920 mRNA. Translation: AAH11920.2.
BC109029 mRNA. Translation: AAI09030.1.
BC109030 mRNA. Translation: AAI09031.1.
BC126899 mRNA. Translation: AAI26900.1. Frameshift.
BC128055 mRNA. Translation: AAI28056.1.
CCDSiCCDS34075.1.
RefSeqiNP_078881.3. NM_024605.3.
UniGeneiHs.368631.

Genome annotation databases

EnsembliENST00000336498; ENSP00000336923; ENSG00000071205.
GeneIDi79658.
KEGGihsa:79658.
UCSCiuc003ilf.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB050785 mRNA. Translation: BAB61771.1 .
BC011920 mRNA. Translation: AAH11920.2 .
BC109029 mRNA. Translation: AAI09030.1 .
BC109030 mRNA. Translation: AAI09031.1 .
BC126899 mRNA. Translation: AAI26900.1 . Frameshift.
BC128055 mRNA. Translation: AAI28056.1 .
CCDSi CCDS34075.1.
RefSeqi NP_078881.3. NM_024605.3.
UniGenei Hs.368631.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2MIO NMR - A 728-786 [» ]
ProteinModelPortali A1A4S6.
SMRi A1A4S6. Positions 2-364, 389-579, 728-786.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 122784. 5 interactions.
IntActi A1A4S6. 1 interaction.
MINTi MINT-203211.
STRINGi 9606.ENSP00000336923.

PTM databases

PhosphoSitei A1A4S6.

Proteomic databases

MaxQBi A1A4S6.
PaxDbi A1A4S6.
PRIDEi A1A4S6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000336498 ; ENSP00000336923 ; ENSG00000071205 .
GeneIDi 79658.
KEGGi hsa:79658.
UCSCi uc003ilf.3. human.

Organism-specific databases

CTDi 79658.
GeneCardsi GC04P148653.
HGNCi HGNC:26099. ARHGAP10.
HPAi HPA039589.
MIMi 609746. gene.
neXtProti NX_A1A4S6.
PharmGKBi PA134904544.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG311191.
HOGENOMi HOG000018767.
HOVERGENi HBG067993.
InParanoidi A1A4S6.
KOi K13736.
OMAi CFDVEAA.
OrthoDBi EOG71VSS5.
PhylomeDBi A1A4S6.
TreeFami TF316851.

Enzyme and pathway databases

Reactomei REACT_11051. Rho GTPase cycle.
REACT_13528. Regulation of PAK-2p34 activity by PS-GAP/RHG10.

Miscellaneous databases

ChiTaRSi ARHGAP10. human.
GenomeRNAii 79658.
NextBioi 68834.
PROi A1A4S6.
SOURCEi Search...

Gene expression databases

ArrayExpressi A1A4S6.
Bgeei A1A4S6.
CleanExi HS_ARHGAP10.
Genevestigatori A1A4S6.

Family and domain databases

Gene3Di 1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProi IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00620. RhoGAP. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
SMARTi SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF48350. SSF48350. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEi PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PKNbeta interacts with the SH3 domains of Graf and a novel Graf related protein, Graf2, which are GTPase activating proteins for Rho family."
    Shibata H., Oishi K., Yamagiwa A., Matsumoto M., Mukai H., Ono Y.
    J. Biochem. 130:23-31(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH PKN3, TISSUE SPECIFICITY, PHOSPHORYLATION, VARIANT VAL-684.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. "Solution NMR structure of SH3 domain 1 of Rho GTPase-activating protein 10 from Homo sapiens, Northeast structural genomics consortium (NESG) target HR9129A."
    Northeast structural genomics consortium (NESG)
    Submitted (JAN-2014) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 728-786.

Entry informationi

Entry nameiRHG10_HUMAN
AccessioniPrimary (citable) accession number: A1A4S6
Secondary accession number(s): A1L0S5
, Q2VPC4, Q2VPC5, Q96EV3, Q96S75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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