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A1A4J1

- PFKAL_BOVIN

UniProt

A1A4J1 - PFKAL_BOVIN

Protein

ATP-dependent 6-phosphofructokinase, liver type

Gene

PFKL

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate. GlcNAcylation by OGT overcomes allosteric regulation By similarity.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251ATP; via amide nitrogenUniRule annotation
    Metal bindingi119 – 1191Magnesium; catalyticUniRule annotation
    Active sitei166 – 1661Proton acceptorUniRule annotation
    Binding sitei201 – 2011Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei264 – 2641SubstrateUniRule annotation
    Binding sitei292 – 2921Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei470 – 4701Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei565 – 5651Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei628 – 6281Allosteric activator fructose 2,6-bisphosphateUniRule annotation
    Binding sitei654 – 6541Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
    Binding sitei734 – 7341Allosteric activator fructose 2,6-bisphosphateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi88 – 892ATPUniRule annotation
    Nucleotide bindingi118 – 1214ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. fructose-6-phosphate binding Source: Ensembl
    4. fructose binding Source: Ensembl
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. fructose 1,6-bisphosphate metabolic process Source: UniProtKB
    3. fructose 6-phosphate metabolic process Source: Ensembl
    4. glycolytic process Source: UniProtKB
    5. negative regulation of insulin secretion Source: Ensembl
    6. protein oligomerization Source: Ensembl
    7. response to glucose Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_224187. Glycolysis.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinase, liver typeUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PFK-L
    Alternative name(s):
    6-phosphofructokinase type B
    Phosphofructo-1-kinase isozyme B
    Short name:
    PFK-B
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:PFKL
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 780779ATP-dependent 6-phosphofructokinase, liver typePRO_0000284440Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Glycosylationi529 – 5291O-linked (GlcNAc)By similarity
    Modified residuei640 – 6401PhosphotyrosineBy similarity
    Modified residuei775 – 7751PhosphoserineBy similarity

    Post-translational modificationi

    GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation By similarity.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiA1A4J1.
    PRIDEiA1A4J1.

    Interactioni

    Subunit structurei

    Homo- and heterotetramers.UniRule annotation

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000014086.

    Structurei

    3D structure databases

    ProteinModelPortaliA1A4J1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 390389N-terminal catalytic PFK domain 1Add
    BLAST
    Regioni164 – 1663Substrate bindingUniRule annotation
    Regioni208 – 2103Substrate bindingUniRule annotation
    Regioni298 – 3014Substrate bindingUniRule annotation
    Regioni391 – 40010Interdomain linker
    Regioni401 – 780380C-terminal regulatory PFK domain 2Add
    BLAST
    Regioni527 – 5315Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni572 – 5743Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
    Regioni660 – 6634Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    GeneTreeiENSGT00390000013209.
    HOGENOMiHOG000200154.
    HOVERGENiHBG000976.
    KOiK00850.
    OMAiVQHGITN.
    OrthoDBiEOG7ZSHV5.
    TreeFamiTF300411.

    Family and domain databases

    HAMAPiMF_03184. Phosphofructokinase_I_E.
    InterProiIPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 2 hits.
    TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1A4J1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVDLEKLR TTGAGKAIGV LTSGGDAQGM NAAVRAVTRM GIYVGAKVFL    50
    IYEGYEGLVE GGENIKQANW LSVSNIIQLG GTVIGSARCK AFTTREGRRA 100
    AAYNLVQRGI TNLCVIGGDG SLTGANIFRS EWGSLLEELV SEGKISEGTA 150
    QTYSHLNIAG LVGSIDNDFC GTDMTIGTDS ALHRIMEVID AITTTAQSHQ 200
    RTFVLEVMGR HCGYLALVSA LASGADWLFI PEAPPEDGWE NFMCERLGET 250
    RSRGSRLNII IIAEGAIDRN GKPISSRYVK DLVVQRLGFD TRVTVLGHVQ 300
    RGGTPSAFDR ILSSKMGMEA VMALLEATPD TPACVVSLSG NQSVRLPLME 350
    CVQMTKEVQK AMDEKRFDEA IQLRGGSFEN NWNIYKLLSH QKISKEKTNF 400
    SLAILNVGAP AAGMNAAVRS AVRSGISQGH TVYVVHDGFE GLAKNQVQEV 450
    SWHDVAGWLG RGGSMLGTKR TLPKGFMEKI VENIRLHNIH ALLVIGGFEA 500
    YEGVLQLVEA RGRYEELCIV MCVIPATISN NVPGTDFSLG SDTAVNAAME 550
    SCDRIKQSAS GTKRRVFIVE TMGGYCGYLA TVTGIAVGAD AAYVFEDPFN 600
    IQDLKANVEH MTEKMKTEIQ RGLVLRNEKC HEHYTTEFLY NLYSSEGKGV 650
    FDCRTNVLGH LQQGGAPTPF DRNYGTKLGV KAIIWMSEKL RAVYRNGRVF 700
    ANASDSACVI GLQKKVVAFS PVTELKKDTD FEHRMPREQW WLNLRLMLKM 750
    LAHYRISMAD YVSGELEHVT RRTLSIETGF 780
    Length:780
    Mass (Da):85,292
    Last modified:January 23, 2007 - v1
    Checksum:iCB081193084B757D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126578 mRNA. Translation: AAI26579.1.
    RefSeqiNP_001073713.1. NM_001080244.2.
    UniGeneiBt.5061.

    Genome annotation databases

    EnsembliENSBTAT00000056305; ENSBTAP00000051545; ENSBTAG00000010658.
    GeneIDi508683.
    KEGGibta:508683.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC126578 mRNA. Translation: AAI26579.1 .
    RefSeqi NP_001073713.1. NM_001080244.2.
    UniGenei Bt.5061.

    3D structure databases

    ProteinModelPortali A1A4J1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000014086.

    Proteomic databases

    PaxDbi A1A4J1.
    PRIDEi A1A4J1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000056305 ; ENSBTAP00000051545 ; ENSBTAG00000010658 .
    GeneIDi 508683.
    KEGGi bta:508683.

    Organism-specific databases

    CTDi 5211.

    Phylogenomic databases

    eggNOGi COG0205.
    GeneTreei ENSGT00390000013209.
    HOGENOMi HOG000200154.
    HOVERGENi HBG000976.
    KOi K00850.
    OMAi VQHGITN.
    OrthoDBi EOG7ZSHV5.
    TreeFami TF300411.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    Reactomei REACT_224187. Glycolysis.

    Miscellaneous databases

    NextBioi 20868629.

    Family and domain databases

    HAMAPi MF_03184. Phosphofructokinase_I_E.
    InterProi IPR009161. 6-phosphofructokinase_euk.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000533. ATP_PFK_euk. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 2 hits.
    TIGRFAMsi TIGR02478. 6PF1K_euk. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Placenta.

    Entry informationi

    Entry nameiPFKAL_BOVIN
    AccessioniPrimary (citable) accession number: A1A4J1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 17, 2007
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3