Reviewed, UniProtKB/Swiss-Prot A1A3N3 (SYM_BIFAA)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BAD_1535

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Monomer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm By similarity HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331783
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00098
	Motif	347 – 351	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	350	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A3N3-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: F66B84A6A929A3BA	FASTA	620	68,694
	10 20 30 40 50 60 MSHILVNVAW PYANGPRHIG HVAGFGVPSD VYARYERMKG NDVLMVSGTD EHGTPILVEA 70 80 90 100 110 120 DKEGVSAQEL ANRYNRVIAK DLCDLGLSYD LFTRTTTGNH EKVVQELFKQ CLENGYIYKG 130 140 150 160 170 180 TQKVAISPST GRTLPDRYIE GTCPICGADG ARGDQCDACG NELDPDELLN PVSKINGETP 190 200 210 220 230 240 RFEETEHFFL DLPALAEANL AWLKTREGWR TNVINFSIGL FKEVKPRAIT RDIDWGIPVP 250 260 270 280 290 300 VKGWIDNPNK KLYVWFDAVI GYLSASIEWA RRKGDPEAWR AWWNDPTTPG YYFMGKDNIT 310 320 330 340 350 360 FHSQIWPSEM LAYNGQGSKG GETGKLGPLN MPEQVVASEF MTMEGKKFSS SRGIVIYVKD 370 380 390 400 410 420 ILARYPVDAV RYYISVAGPE SSDSDFTWAE FVRHNNEELA ASWGNLVNRV ANLINKNFGE 430 440 450 460 470 480 IPAFDEASAT DEDRALLAET KAAFETVGGL IENHRQKNAL SEAMRVVGDI NKYISATEPW 490 500 510 520 530 540 KIKDNPARLG TVLHTAAQAV SDANHLLAPF LPHSAQKVWE ALGGTGVFSP LPRLEEVEDL 550 560 570 580 590 600 DKPGFTYPII TGDYKLGETV HPWASEPIVA GTSVPKPHPI FAKIPPEAVE EELARFDAEL 610 620 KARREAEAAR LAAEKAKLEG

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF40316.1.
RefSeq	YP_910398.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1A3N3.
Genome annotation databases
GeneID	4556930.
GenomeReviews	Gene locus BAD_1535 in contig AP009256_GR.
KEGG	bad:BAD_1535.
NMPDR	fig|1680.3.peg.1124.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HBG721667.
OMA	KLISKCE.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BIFAA

Accession

Primary (citable) accession number: A1A3N3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)