Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase BgaB

Gene

bgaB

Organism
Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of transgalactooligosaccharides (TOS). Highly active towards Gal(beta1-4)Gal and Gal(beta1-4)-Gal-containing oligosaccharides. Low activity towards Gal(beta1-3)Gal, lactose and Gal(beta1-3)GalOMe. No activity towards Gal(beta1-6)Gal, Gal(beta1-4)Man, Gal(alpha1-4)Gal, Gal(alpha1-3)Gal(beta1-4)Gal, lactulose, 3'fucosyllactose, lacto-N-fucopentaose I, lacto-N-fucopentaose II, cellobiose, maltose or sucrose. No transglycosylation activity is found at high substrate concentrations (100 mg/ml) and only low transglycosylation activity at lower substrate concentrations (10 mg/ml).2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.2 Publications

Enzyme regulationi

Inhibited by high substrate concentrations (100 mg/ml). No effect on activity with various EDTA concentrations (0-1 mM). 20-fold higher activity when cells grown on TOS than when cells grown on galactose, glucose and lactose.2 Publications

Kineticsi

  1. KM=60 mM for Gal(beta1-4)Gal (at 37 degrees Celsius and pH 6.0)2 Publications
  2. KM=2.2 mM for p-nitrophenyl-beta-D-galactopyranoside (PNPG) (at 40 degrees Celsius and pH 6.0)2 Publications
  3. KM=2.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)2 Publications
  4. KM=4.0 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)2 Publications
  5. KM=3.7 mM for beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
  6. KM=6.4 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
  7. KM=5.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
  1. Vmax=1.129 µmol/min/mg enzyme with Gal(beta1-4)Gal as substrate (at 37 degrees Celsius and pH 6.0)2 Publications

pH dependencei

Optimum is pH 6.0 using PNPG or TOS as substrate. Not active below pH 5.2 Publications

Temperature dependencei

Optimum temperature is 50 degrees Celsius using PNPG as substrate (PubMed:15480628). Optimum temperature is 35 degrees Celsius using TOS as substrate (PubMed:10742215). Stable at 40 degrees Celsius. Half-life time at 50 degrees Celsius is 10 minutes.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122SubstrateBy similarity1
Binding sitei160SubstrateBy similarity1
Active sitei161Proton donorBy similarity1
Active sitei320NucleophileBy similarity1
Binding sitei328SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH42 Glycoside Hydrolase Family 42

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaB (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Beta-Gal II
Gene namesi
Name:bgaB
Synonyms:bgaLII
Ordered Locus Names:BAD_1401
OrganismiBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Taxonomic identifieri367928 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000008702 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004076832 – 700Beta-galactosidase BgaBAdd BLAST699

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Trimer (PubMed:15480628). Tetramer (PubMed:10742215).2 Publications

Protein-protein interaction databases

STRINGi367928.BAD_1401

Structurei

Secondary structure

1700
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi412 – 417Combined sources6
Helixi419 – 425Combined sources7
Helixi437 – 450Combined sources14
Beta strandi456 – 459Combined sources4
Beta strandi468 – 474Combined sources7
Helixi480 – 491Combined sources12
Beta strandi495 – 499Combined sources5
Beta strandi502 – 505Combined sources4
Beta strandi514 – 517Combined sources4
Helixi519 – 522Combined sources4
Helixi523 – 528Combined sources6
Beta strandi530 – 534Combined sources5
Beta strandi549 – 556Combined sources8
Beta strandi564 – 569Combined sources6
Beta strandi580 – 587Combined sources8
Helixi588 – 592Combined sources5
Beta strandi599 – 606Combined sources8
Beta strandi609 – 614Combined sources6
Helixi620 – 630Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5VYMX-ray2.46A/B409-638[»]
SMRiA1A399
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 371Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Curated

Phylogenomic databases

eggNOGiENOG4105C4C Bacteria
COG1874 LUCA
HOGENOMiHOG000117811
KOiK12308
OrthoDBiPOG091H0719

Family and domain databases

Gene3Di3.40.50.880, 1 hit
InterProiView protein in InterPro
IPR013738 Beta_galactosidase_Trimer
IPR029062 Class_I_gatase-like
IPR003476 Glyco_hydro_42
IPR013529 Glyco_hydro_42_N
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR36447 PTHR36447, 1 hit
PfamiView protein in Pfam
PF02449 Glyco_hydro_42, 1 hit
PF08532 Glyco_hydro_42M, 1 hit
PIRSFiPIRSF001084 B-galactosidase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF52317 SSF52317, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1A399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARRNFEWP ELLTADGRGI AFGGDYNPDQ WSEDIWDDDI RLMKQAGVNT
60 70 80 90 100
VALAIFSWDR IQPTEDRWDF GWLDRIIDKL GNAGIVVDLA SATATAPLWL
110 120 130 140 150
YESHPEVLPR DKYGHPVNAG SRQSWSPTSP VFKEYALTLC RKLAERYGTN
160 170 180 190 200
PYVTAWHMGN EYGWNNREDY SDNALEAFRA WCRRKYGTID ALNQAWGTTF
210 220 230 240 250
WGQEMNGFDE VLIPRFMGAD SMVNPGQKLD FERFGNDMLL DFYKAERDAI
260 270 280 290 300
AEICPDKPFT TNFMVSTDQC CMDYAAWAKE VNFVSNDHYF HEGESHLDEL
310 320 330 340 350
ACSDALMDSL ALGKPWYVME HSTSAVQWKP LNTRKRKGET VRDSLAHVAM
360 370 380 390 400
GADAINFFQW RASAFGAEAF HSAMVPHAGE DTKLFRQVCE LGASLHTLAD
410 420 430 440 450
AGVQGTELAH SDTAILFSAE SEWATRSQTL PSMKLNHWHD VRDWYRAFLD
460 470 480 490 500
AGSRADIVPL AYDWSSYKTV VLPTVLILSA ADTQRLADFA AAGGRVVVGY
510 520 530 540 550
ATGLIDEHFH TWLGGYPGAG DGLLRSMLGV RGEEFNILGA EAEGEPGEIR
560 570 580 590 600
LSSADDSAAL DGTTTRLWQN DVNVTGEHAQ VLATYAGEEA DEWELDGTAA
610 620 630 640 650
VTRNPYGSGE AYFVGCDLDV ADLTKLVRAY LAASSQENAD VLHTVRASAD
660 670 680 690 700
ATFDFYLPRG KKTVELQGIE GEPVILFQTD REEKPGSYTV RRNGVLVVRR
Length:700
Mass (Da):78,025
Last modified:May 3, 2011 - v2
Checksum:i9EDB064761188A52
GO

Sequence cautioni

The sequence BAF40182 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213 – 230IPRFM…GQKLD → HPTVHGRRLRWSTPARSST in AAR24113 (PubMed:15480628).CuratedAdd BLAST18
Sequence conflicti536N → T in AAR24113 (PubMed:15480628).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY359872 Genomic DNA Translation: AAR24113.1
AP009256 Genomic DNA Translation: BAF40182.1 Different initiation.
RefSeqiWP_041777416.1, NC_008618.1

Genome annotation databases

EnsemblBacteriaiBAF40182; BAF40182; BAD_1401
GeneIDi4557550
KEGGibad:BAD_1401

Similar proteinsi

Entry informationi

Entry nameiBGAL_BIFAA
AccessioniPrimary (citable) accession number: A1A399
Secondary accession number(s): Q5J883
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 58 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health