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Protein

Beta-galactosidase BgaB

Gene

bgaB

Organism
Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the hydrolysis of transgalactooligosaccharides (TOS). Highly active towards Gal(beta1-4)Gal and Gal(beta1-4)-Gal-containing oligosaccharides. Low activity towards Gal(beta1-3)Gal, lactose and Gal(beta1-3)GalOMe. No activity towards Gal(beta1-6)Gal, Gal(beta1-4)Man, Gal(alpha1-4)Gal, Gal(alpha1-3)Gal(beta1-4)Gal, lactulose, 3'fucosyllactose, lacto-N-fucopentaose I, lacto-N-fucopentaose II, cellobiose, maltose or sucrose. No transglycosylation activity is found at high substrate concentrations (100 mg/ml) and only low transglycosylation activity at lower substrate concentrations (10 mg/ml).2 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.2 Publications

Enzyme regulationi

Inhibited by high substrate concentrations (100 mg/ml). No effect on activity with various EDTA concentrations (0-1 mM). 20-fold higher activity when cells grown on TOS than when cells grown on galactose, glucose and lactose.2 Publications

Kineticsi

  1. KM=60 mM for Gal(beta1-4)Gal (at 37 degrees Celsius and pH 6.0)2 Publications
  2. KM=2.2 mM for p-nitrophenyl-beta-D-galactopyranoside (PNPG) (at 40 degrees Celsius and pH 6.0)2 Publications
  3. KM=2.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)2 Publications
  4. KM=4.0 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)2 Publications
  5. KM=3.7 mM for beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
  6. KM=6.4 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
  7. KM=5.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
  1. Vmax=1.129 µmol/min/mg enzyme with Gal(beta1-4)Gal as substrate (at 37 degrees Celsius and pH 6.0)2 Publications

pH dependencei

Optimum is pH 6.0 using PNPG or TOS as substrate. Not active below pH 5.2 Publications

Temperature dependencei

Optimum temperature is 50 degrees Celsius using PNPG as substrate (PubMed:15480628). Optimum temperature is 35 degrees Celsius using TOS as substrate (PubMed:10742215). Stable at 40 degrees Celsius. Half-life time at 50 degrees Celsius is 10 minutes.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122SubstrateBy similarity1
Binding sitei160SubstrateBy similarity1
Active sitei161Proton donorBy similarity1
Active sitei320NucleophileBy similarity1
Binding sitei328SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaB (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Beta-Gal II
Gene namesi
Name:bgaB
Synonyms:bgaLII
Ordered Locus Names:BAD_1401
OrganismiBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Taxonomic identifieri367928 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000008702 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00004076832 – 700Beta-galactosidase BgaBAdd BLAST699

Post-translational modificationi

The N-terminus is blocked.

Interactioni

Subunit structurei

Trimer (PubMed:15480628). Tetramer (PubMed:10742215).2 Publications

Protein-protein interaction databases

STRINGi367928.BAD_1401.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 371Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Curated

Phylogenomic databases

eggNOGiENOG4105C4C. Bacteria.
COG1874. LUCA.
HOGENOMiHOG000117811.
KOiK12308.
OrthoDBiPOG091H0719.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A1A399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARRNFEWP ELLTADGRGI AFGGDYNPDQ WSEDIWDDDI RLMKQAGVNT
60 70 80 90 100
VALAIFSWDR IQPTEDRWDF GWLDRIIDKL GNAGIVVDLA SATATAPLWL
110 120 130 140 150
YESHPEVLPR DKYGHPVNAG SRQSWSPTSP VFKEYALTLC RKLAERYGTN
160 170 180 190 200
PYVTAWHMGN EYGWNNREDY SDNALEAFRA WCRRKYGTID ALNQAWGTTF
210 220 230 240 250
WGQEMNGFDE VLIPRFMGAD SMVNPGQKLD FERFGNDMLL DFYKAERDAI
260 270 280 290 300
AEICPDKPFT TNFMVSTDQC CMDYAAWAKE VNFVSNDHYF HEGESHLDEL
310 320 330 340 350
ACSDALMDSL ALGKPWYVME HSTSAVQWKP LNTRKRKGET VRDSLAHVAM
360 370 380 390 400
GADAINFFQW RASAFGAEAF HSAMVPHAGE DTKLFRQVCE LGASLHTLAD
410 420 430 440 450
AGVQGTELAH SDTAILFSAE SEWATRSQTL PSMKLNHWHD VRDWYRAFLD
460 470 480 490 500
AGSRADIVPL AYDWSSYKTV VLPTVLILSA ADTQRLADFA AAGGRVVVGY
510 520 530 540 550
ATGLIDEHFH TWLGGYPGAG DGLLRSMLGV RGEEFNILGA EAEGEPGEIR
560 570 580 590 600
LSSADDSAAL DGTTTRLWQN DVNVTGEHAQ VLATYAGEEA DEWELDGTAA
610 620 630 640 650
VTRNPYGSGE AYFVGCDLDV ADLTKLVRAY LAASSQENAD VLHTVRASAD
660 670 680 690 700
ATFDFYLPRG KKTVELQGIE GEPVILFQTD REEKPGSYTV RRNGVLVVRR
Length:700
Mass (Da):78,025
Last modified:May 3, 2011 - v2
Checksum:i9EDB064761188A52
GO

Sequence cautioni

The sequence BAF40182 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti213 – 230IPRFM…GQKLD → HPTVHGRRLRWSTPARSST in AAR24113 (PubMed:15480628).CuratedAdd BLAST18
Sequence conflicti536N → T in AAR24113 (PubMed:15480628).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY359872 Genomic DNA. Translation: AAR24113.1.
AP009256 Genomic DNA. Translation: BAF40182.1. Different initiation.
RefSeqiWP_041777416.1. NC_008618.1.

Genome annotation databases

EnsemblBacteriaiBAF40182; BAF40182; BAD_1401.
GeneIDi4557550.
KEGGibad:BAD_1401.
PATRICi21102249. VBIBifAdo27973_1479.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY359872 Genomic DNA. Translation: AAR24113.1.
AP009256 Genomic DNA. Translation: BAF40182.1. Different initiation.
RefSeqiWP_041777416.1. NC_008618.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi367928.BAD_1401.

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF40182; BAF40182; BAD_1401.
GeneIDi4557550.
KEGGibad:BAD_1401.
PATRICi21102249. VBIBifAdo27973_1479.

Phylogenomic databases

eggNOGiENOG4105C4C. Bacteria.
COG1874. LUCA.
HOGENOMiHOG000117811.
KOiK12308.
OrthoDBiPOG091H0719.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_BIFAA
AccessioniPrimary (citable) accession number: A1A399
Secondary accession number(s): Q5J883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: November 2, 2016
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.