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A1A399

- BGAL_BIFAA

UniProt

A1A399 - BGAL_BIFAA

Protein

Beta-galactosidase BgaB

Gene

bgaB

Organism
Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 2 (03 May 2011)
      Previous versions | rss
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    Functioni

    Involved in the hydrolysis of transgalactooligosaccharides (TOS). Highly active towards Gal(beta1-4)Gal and Gal(beta1-4)-Gal-containing oligosaccharides. Low activity towards Gal(beta1-3)Gal, lactose and Gal(beta1-3)GalOMe. No activity towards Gal(beta1-6)Gal, Gal(beta1-4)Man, Gal(alpha1-4)Gal, Gal(alpha1-3)Gal(beta1-4)Gal, lactulose, 3'fucosyllactose, lacto-N-fucopentaose I, lacto-N-fucopentaose II, cellobiose, maltose or sucrose. No transglycosylation activity is found at high substrate concentrations (100 mg/ml) and only low transglycosylation activity at lower substrate concentrations (10 mg/ml).2 Publications

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.2 Publications

    Enzyme regulationi

    Inhibited by high substrate concentrations (100 mg/ml). No effect on activity with various EDTA concentrations (0-1 mM). 20-fold higher activity when cells grown on TOS than when cells grown on galactose, glucose and lactose.2 Publications

    Kineticsi

    1. KM=60 mM for Gal(beta1-4)Gal (at 37 degrees Celsius and pH 6.0)2 Publications
    2. KM=2.2 mM for p-nitrophenyl-beta-D-galactopyranoside (PNPG) (at 40 degrees Celsius and pH 6.0)2 Publications
    3. KM=2.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)2 Publications
    4. KM=4.0 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)2 Publications
    5. KM=3.7 mM for beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
    6. KM=6.4 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications
    7. KM=5.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)2 Publications

    Vmax=1.129 µmol/min/mg enzyme with Gal(beta1-4)Gal as substrate (at 37 degrees Celsius and pH 6.0)2 Publications

    pH dependencei

    Optimum is pH 6.0 using PNPG or TOS as substrate. Not active below pH 5.2 Publications

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius using PNPG as substrate (PubMed:15480628). Optimum temperature is 35 degrees Celsius using TOS as substrate (PubMed:10742215). Stable at 40 degrees Celsius. Half-life time at 50 degrees Celsius is 10 minutes.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei122 – 1221SubstrateBy similarity
    Binding sitei160 – 1601SubstrateBy similarity
    Active sitei161 – 1611Proton donorBy similarity
    Active sitei320 – 3201NucleophileBy similarity
    Binding sitei328 – 3281SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciBADO367928:GHPT-1457-MONOMER.

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaB (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Beta-Gal II
    Gene namesi
    Name:bgaB
    Synonyms:bgaLII
    Ordered Locus Names:BAD_1401
    OrganismiBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
    Taxonomic identifieri367928 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium
    ProteomesiUP000008702: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 700699Beta-galactosidase BgaBPRO_0000407683Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Interactioni

    Subunit structurei

    Trimer (PubMed:15480628). Tetramer (PubMed:10742215).2 Publications

    Protein-protein interaction databases

    STRINGi367928.BAD_1401.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni368 – 3714Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Curated

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000117811.
    KOiK12308.
    OrthoDBiEOG6GTZGG.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A1A399-1 [UniParc]FASTAAdd to Basket

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    MSARRNFEWP ELLTADGRGI AFGGDYNPDQ WSEDIWDDDI RLMKQAGVNT    50
    VALAIFSWDR IQPTEDRWDF GWLDRIIDKL GNAGIVVDLA SATATAPLWL 100
    YESHPEVLPR DKYGHPVNAG SRQSWSPTSP VFKEYALTLC RKLAERYGTN 150
    PYVTAWHMGN EYGWNNREDY SDNALEAFRA WCRRKYGTID ALNQAWGTTF 200
    WGQEMNGFDE VLIPRFMGAD SMVNPGQKLD FERFGNDMLL DFYKAERDAI 250
    AEICPDKPFT TNFMVSTDQC CMDYAAWAKE VNFVSNDHYF HEGESHLDEL 300
    ACSDALMDSL ALGKPWYVME HSTSAVQWKP LNTRKRKGET VRDSLAHVAM 350
    GADAINFFQW RASAFGAEAF HSAMVPHAGE DTKLFRQVCE LGASLHTLAD 400
    AGVQGTELAH SDTAILFSAE SEWATRSQTL PSMKLNHWHD VRDWYRAFLD 450
    AGSRADIVPL AYDWSSYKTV VLPTVLILSA ADTQRLADFA AAGGRVVVGY 500
    ATGLIDEHFH TWLGGYPGAG DGLLRSMLGV RGEEFNILGA EAEGEPGEIR 550
    LSSADDSAAL DGTTTRLWQN DVNVTGEHAQ VLATYAGEEA DEWELDGTAA 600
    VTRNPYGSGE AYFVGCDLDV ADLTKLVRAY LAASSQENAD VLHTVRASAD 650
    ATFDFYLPRG KKTVELQGIE GEPVILFQTD REEKPGSYTV RRNGVLVVRR 700
    Length:700
    Mass (Da):78,025
    Last modified:May 3, 2011 - v2
    Checksum:i9EDB064761188A52
    GO

    Sequence cautioni

    The sequence BAF40182.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti213 – 23018IPRFM…GQKLD → HPTVHGRRLRWSTPARSST in AAR24113. (PubMed:15480628)CuratedAdd
    BLAST
    Sequence conflicti536 – 5361N → T in AAR24113. (PubMed:15480628)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY359872 Genomic DNA. Translation: AAR24113.1.
    AP009256 Genomic DNA. Translation: BAF40182.1. Different initiation.
    RefSeqiYP_910264.1. NC_008618.1.

    Genome annotation databases

    EnsemblBacteriaiBAF40182; BAF40182; BAD_1401.
    GeneIDi4557550.
    KEGGibad:BAD_1401.
    PATRICi21102249. VBIBifAdo27973_1479.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY359872 Genomic DNA. Translation: AAR24113.1 .
    AP009256 Genomic DNA. Translation: BAF40182.1 . Different initiation.
    RefSeqi YP_910264.1. NC_008618.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 367928.BAD_1401.

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF40182 ; BAF40182 ; BAD_1401 .
    GeneIDi 4557550.
    KEGGi bad:BAD_1401.
    PATRICi 21102249. VBIBifAdo27973_1479.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000117811.
    KOi K12308.
    OrthoDBi EOG6GTZGG.

    Enzyme and pathway databases

    BioCyci BADO367928:GHPT-1457-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "beta-galactosidase from Bifidobacterium adolescentis DSM20083 prefers beta(1,4)-galactosides over lactose."
      Hinz S.W., van den Brock L.A., Beldman G., Vincken J.P., Voragen A.G.
      Appl. Microbiol. Biotechnol. 66:276-284(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16; 20-29; 455-463 AND 535-546, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBSTRATE SPECIFICITY.
      Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.
    2. "Bifidobacterium adolescentis complete genome sequence."
      Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K.
      Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.
    3. "Characterization of a novel beta-galactosidase from Bifidobacterium adolescentis DSM 20083 active towards transgalactooligosaccharides."
      Van Laere K.M., Abee T., Schols H.A., Beldman G., Voragen A.G.
      Appl. Environ. Microbiol. 66:1379-1384(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
      Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

    Entry informationi

    Entry nameiBGAL_BIFAA
    AccessioniPrimary (citable) accession number: A1A399
    Secondary accession number(s): Q5J883
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: May 3, 2011
    Last modified: October 1, 2014
    This is version 43 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3