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A1A399 (BGAL_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase BgaB

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Beta-Gal II
Gene names
Name:bgaB
Synonyms:bgaLII
Ordered Locus Names:BAD_1401
OrganismBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]
Taxonomic identifier367928 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length700 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the hydrolysis of transgalactooligosaccharides (TOS). Highly active towards Gal(beta1-4)Gal and Gal(beta1-4)-Gal-containing oligosaccharides. Low activity towards Gal(beta1-3)Gal, lactose and Gal(beta1-3)GalOMe. No activity towards Gal(beta1-6)Gal, Gal(beta1-4)Man, Gal(alpha1-4)Gal, Gal(alpha1-3)Gal(beta1-4)Gal, lactulose, 3'fucosyllactose, lacto-N-fucopentaose I, lacto-N-fucopentaose II, cellobiose, maltose or sucrose. No transglycosylation activity is found at high substrate concentrations (100 mg/ml) and only low transglycosylation activity at lower substrate concentrations (10 mg/ml). Ref.1 Ref.3

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.1 Ref.3

Enzyme regulation

Inhibited by high substrate concentrations (100 mg/ml). No effect on activity with various EDTA concentrations (0-1 mM). 20-fold higher activity when cells grown on TOS than when cells grown on galactose, glucose and lactose. Ref.1 Ref.3

Subunit structure

Trimer (Ref.1). Tetramer (Ref.3).

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Biophysicochemical properties

Kinetic parameters:

KM=60 mM for Gal(beta1-4)Gal (at 37 degrees Celsius and pH 6.0) Ref.1 Ref.3

KM=2.2 mM for p-nitrophenyl-beta-D-galactopyranoside (PNPG) (at 40 degrees Celsius and pH 6.0)

KM=2.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)

KM=4.0 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Glcp (at 40 degrees Celsius and pH 6.0)

KM=3.7 mM for beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)

KM=6.4 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)

KM=5.2 mM for beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-beta-D-Galp-(1->4)-D-Galp (at 40 degrees Celsius and pH 6.0)

Vmax=1.129 µmol/min/mg enzyme with Gal(beta1-4)Gal as substrate (at 37 degrees Celsius and pH 6.0)

pH dependence:

Optimum is pH 6.0 using PNPG or TOS as substrate. Not active below pH 5.

Temperature dependence:

Optimum temperature is 50 degrees Celsius using PNPG as substrate (Ref.1). Optimum temperature is 35 degrees Celsius using TOS as substrate (Ref.3). Stable at 40 degrees Celsius. Half-life time at 50 degrees Celsius is 10 minutes.

Sequence caution

The sequence BAF40182.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 700699Beta-galactosidase BgaB
PRO_0000407683

Regions

Region368 – 3714Substrate binding By similarity

Sites

Active site1611Proton donor By similarity
Active site3201Nucleophile By similarity
Binding site1221Substrate By similarity
Binding site1601Substrate By similarity
Binding site3281Substrate By similarity

Experimental info

Sequence conflict213 – 23018IPRFM…GQKLD → HPTVHGRRLRWSTPARSST in AAR24113. Ref.1
Sequence conflict5361N → T in AAR24113. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A1A399 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 9EDB064761188A52

FASTA70078,025
        10         20         30         40         50         60 
MSARRNFEWP ELLTADGRGI AFGGDYNPDQ WSEDIWDDDI RLMKQAGVNT VALAIFSWDR 

        70         80         90        100        110        120 
IQPTEDRWDF GWLDRIIDKL GNAGIVVDLA SATATAPLWL YESHPEVLPR DKYGHPVNAG 

       130        140        150        160        170        180 
SRQSWSPTSP VFKEYALTLC RKLAERYGTN PYVTAWHMGN EYGWNNREDY SDNALEAFRA 

       190        200        210        220        230        240 
WCRRKYGTID ALNQAWGTTF WGQEMNGFDE VLIPRFMGAD SMVNPGQKLD FERFGNDMLL 

       250        260        270        280        290        300 
DFYKAERDAI AEICPDKPFT TNFMVSTDQC CMDYAAWAKE VNFVSNDHYF HEGESHLDEL 

       310        320        330        340        350        360 
ACSDALMDSL ALGKPWYVME HSTSAVQWKP LNTRKRKGET VRDSLAHVAM GADAINFFQW 

       370        380        390        400        410        420 
RASAFGAEAF HSAMVPHAGE DTKLFRQVCE LGASLHTLAD AGVQGTELAH SDTAILFSAE 

       430        440        450        460        470        480 
SEWATRSQTL PSMKLNHWHD VRDWYRAFLD AGSRADIVPL AYDWSSYKTV VLPTVLILSA 

       490        500        510        520        530        540 
ADTQRLADFA AAGGRVVVGY ATGLIDEHFH TWLGGYPGAG DGLLRSMLGV RGEEFNILGA 

       550        560        570        580        590        600 
EAEGEPGEIR LSSADDSAAL DGTTTRLWQN DVNVTGEHAQ VLATYAGEEA DEWELDGTAA 

       610        620        630        640        650        660 
VTRNPYGSGE AYFVGCDLDV ADLTKLVRAY LAASSQENAD VLHTVRASAD ATFDFYLPRG 

       670        680        690        700 
KKTVELQGIE GEPVILFQTD REEKPGSYTV RRNGVLVVRR 

« Hide

References

« Hide 'large scale' references
[1]"beta-galactosidase from Bifidobacterium adolescentis DSM20083 prefers beta(1,4)-galactosides over lactose."
Hinz S.W., van den Brock L.A., Beldman G., Vincken J.P., Voragen A.G.
Appl. Microbiol. Biotechnol. 66:276-284(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16; 20-29; 455-463 AND 535-546, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBSTRATE SPECIFICITY.
Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.
[2]"Bifidobacterium adolescentis complete genome sequence."
Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.
[3]"Characterization of a novel beta-galactosidase from Bifidobacterium adolescentis DSM 20083 active towards transgalactooligosaccharides."
Van Laere K.M., Abee T., Schols H.A., Beldman G., Voragen A.G.
Appl. Environ. Microbiol. 66:1379-1384(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION.
Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY359872 Genomic DNA. Translation: AAR24113.1.
AP009256 Genomic DNA. Translation: BAF40182.1. Different initiation.
RefSeqYP_910264.1. NC_008618.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING367928.BAD_1401.

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF40182; BAF40182; BAD_1401.
GeneID4557550.
KEGGbad:BAD_1401.
PATRIC21102249. VBIBifAdo27973_1479.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000117811.
KOK12308.
OrthoDBEOG6GTZGG.

Enzyme and pathway databases

BioCycBADO367928:GHPT-1457-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProIPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL_BIFAA
AccessionPrimary (citable) accession number: A1A399
Secondary accession number(s): Q5J883
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 3, 2011
Last modified: June 11, 2014
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries