Reviewed, UniProtKB/Swiss-Prot A1A2I0 (LSPA_BIFAA)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	BAD_1132

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000038780
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	68 – 88	21	Potential
	Transmembrane	91 – 111	21	Potential
	Transmembrane	141 – 161	21	Potential
Sites
	Active site	112	1	By similarity
	Active site	140	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A2I0-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 6DF6DC1F050E1B9C	FASTA	187	19,855
	10 20 30 40 50 60 MKNDQRRLRD RVAVFACIAI AAIAIDQLTK MWALSALADG RTIRVIPGLL SLTLVRNPGA 70 80 90 100 110 120 SLGMGSGMTW LISLLAMAAC VALVVLAVRT ISMKWTVLFA FAFAGAFGNL IDRVIYAEGF 130 140 150 160 170 180 LNGKVVDFLN YGWSIGNVAD IFLMLAGVAA VLLLFLGEPF SQKDLDEANG KTLGDDANAT DDGAKAA

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009256 Genomic DNA. Translation: BAF39913.1.
RefSeq	YP_909995.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1A2I0.
Protein family/group databases
MEROPS	A08.001.
Genome annotation databases
GeneID	4556353.
GenomeReviews	Gene locus BAD_1132 in contig AP009256_GR.
KEGG	bad:BAD_1132.
NMPDR	fig|1680.3.peg.155.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VVAKLEH.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProDom	PD004304. Peptidase_A8. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_BIFAA

Accession

Primary (citable) accession number: A1A2I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)