Reviewed, UniProtKB/Swiss-Prot A1A2G3 (LEXA_BIFAA)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	BAD_1115

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	LexA repressor HAMAP MF_00015		PRO_0000322715
Regions
	DNA binding	64 – 84	21	H-T-H motif By similarity
Sites
	Active site	181	1	For autocatalytic cleavage activity By similarity
	Active site	218	1	For autocatalytic cleavage activity By similarity
	Site	146 – 147	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A2G3-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 413955312B4982DD	FASTA	257	28,169
	10 20 30 40 50 60 MFDFIGVSRY ASKELRKEPI VSTIPFTPKK SDERPDESTL TDRQRKVLDA IKTHLAKQGF 70 80 90 100 110 120 APSFREIGEA AGLKSPSSVK HQLQVLDEKG FIRMNANKGR AIEVVNLNDE EPNGKVAQVI 130 140 150 160 170 180 PFPSQDDACG SIMASHDVPL VGRIAAGVPI TAEQHVDDVM RLPERLTGTG NLFMLEVHGD 190 200 210 220 230 240 SMIDAAICDG DFVVVREQNS AENGDIVAAL LDDEATVKTF RKDHGHVWLI PHNPAYSPID 250 GTHAEIMGKV VTVLRKI

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39896.1.
RefSeq	YP_909978.1.
3D structure databases
SMR	A1A2G3. Positions 138-256.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A2G3.
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	4556287.
GenomeReviews	Gene locus BAD_1115 in contig AP009256_GR.
KEGG	bad:BAD_1115.
NMPDR	fig|1680.3.peg.138.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
OMA	KVIGVFR.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BIFAA

Accession

Primary (citable) accession number: A1A2G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)