A1A2F1 (MURD_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	BAD_1103

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130829
Regions
	Nucleotide binding	110 – 116	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1A2F1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: EF6C98EA60E4583D	FASTA	479	51,022
	10 20 30 40 50 60 MDMNMDGKTV IVAGLGVSGQ SMMEVLGSRA GRVLGVDEKK PDADLHSFDQ IDWDNTDMVM 70 80 90 100 110 120 TSPVFNPRTP FILEAQKRNI PVMSEVELAW QLRVNSNTTG KPAQWIGITG TNGKTSTTEM 130 140 150 160 170 180 TSEMLTACGL AAPAVGNIGK AVSHAAVDPA NDVLCVELSS FQLHFTYSLE LDCAAITNIA 190 200 210 220 230 240 DDHLDWHGGI ENYAADKAKV FHNVKKALVY NADDERVTKL AFAAQTAPEC RRIGFTLAEP 250 260 270 280 290 300 KDGQIGVKDG WIVDMSGIAG GEAGKPFQVA KVTDFTHLTE PDGTVYPHLL ADALTALALV 310 320 330 340 350 360 LGLGADKDKA LAALKQFTPG GHRIQTVAVA KTSDGGTIRF VDDSKATNAH AAKASLNSFA 370 380 390 400 410 420 DKSVVWIAGG LAKGSRFEQL VADQARTIKA AIIIGKDQQP MLDAFKASAP DIPMSIIDPT 430 440 450 460 470 DNDTVMARAI DAAGTYAQSG DVVLMAPACA SMDQFKSYAD RGNQFAQQAQ RWVNEHGEA

References

[1]

"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39884.1.
RefSeq	YP_909966.1. NC_008618.1.
3D structure databases
ProteinModelPortal	A1A2F1.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A2F1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4556374.
GenomeReviews	Gene locus BAD_1103 in contig AP009256_GR.
KEGG	bad:BAD_1103.
NMPDR	fig|1680.3.peg.128.
PATRIC	21101637. VBIBifAdo27973_1182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	LERHKTL.
ProtClustDB	CLSK573012.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BIFAA

Accession

Primary (citable) accession number: A1A2F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program