Reviewed, UniProtKB/Swiss-Prot A1A2E8 (MURC_BIFAA)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BAD_1100

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable HAMAP MF_00046.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004313
Regions
	Nucleotide binding	136 – 142	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1A2E8-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 5E833F594F7BB682	FASTA	521	55,602
	10 20 30 40 50 60 MSDNQREAGT IILDPTHASF APEETVHDLG ATHFIGIGGA GMSVLAEMLH EQGVEVDGSD 70 80 90 100 110 120 REPSAKTDRL QSLGITVEFG QQAKNVEGKN TVVFSSAIKP DNPEIVAAHE AGERIVHRSD 130 140 150 160 170 180 ILALLMNAKR AVTVAGAHGK TTTSSMLAHI LVNAGEGELA DPSYAIGGSI QGKDGAILDG 190 200 210 220 230 240 GHAGKGNVLV AEADESDGSF AKYHPQIAII TNSEADHLDH YGTQDNYRAA FVDHAGHATK 250 260 270 280 290 300 AVIMCGDDEG NLAVLRALDA TVAGRTIVYS TRNAAELGDL NGATLVRIES ESETAESGAE 310 320 330 340 350 360 HFTLHIPGGL IGEEERRIAV TLTVPGIHNA RNASAAIIAA ALLGMDVERA SAAVTSFLGA 370 380 390 400 410 420 ARRFQVRGTV SQVTVVDDYA HHPTEIAALL DAARRRYPQS KIRVIFQPHL FSRTRFFSSE 430 440 450 460 470 480 FAQALAKADD VIVTGIFPAR EKQEDFPDVT PATIVDEALK LEHEPAKDWI RGVEDMHTAA 490 500 510 520 QMMVMRAHHG DVIFTVGAGD ITQMDEVILH ALEAHRWDCE G

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39881.1.
RefSeq	YP_909963.1.
3D structure databases
SMR	A1A2E8. Positions 30-512.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A2E8.
Genome annotation databases
GeneID	4556373.
GenomeReviews	Gene locus BAD_1100 in contig AP009256_GR.
KEGG	bad:BAD_1100.
NMPDR	fig|1680.3.peg.127.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BIFAA

Accession

Primary (citable) accession number: A1A2E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)