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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:BAD_1031
OrganismiBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Taxonomic identifieri367928 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000008702 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003770851 – 336tRNA dimethylallyltransferaseAdd BLAST336

Proteomic databases

PRIDEiA1A279.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei115Interaction with substrate tRNAUniRule annotation1
Sitei136Interaction with substrate tRNAUniRule annotation1

Protein-protein interaction databases

STRINGi367928.BAD_1031.

Structurei

3D structure databases

ProteinModelPortaliA1A279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 26Substrate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

A1A279-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSATESKPE TRKVVSIVGP TASGKTGLGI AIAKALEAKG EQAEIVNADA
60 70 80 90 100
YQMYKGMDIG TAKASPEEQA EVRHHLIDII EPDDAMSVAR FQEIARAKIA
110 120 130 140 150
ELQAREVRPI LVGGSGLYAR AAIDDISFPG TDPEVRKRLE EREKVEGAGA
160 170 180 190 200
LFDELKTKDP EAAARMDPHN PRRTIRALEV IEVTGRPYSA SLPHYRYVIP
210 220 230 240 250
TVQIGLDLPR EELDRRIDIR TKQMLENGFV EEVERIRPRL GITAGKALGY
260 270 280 290 300
QQVVDYLDGL CDLNDTFMSI AQKTKRLARK QMGWFGRDPR IHWLQALNPA
310 320 330
LLGNAMAIIE HADAGDYDAI DAQADAYTQH HLGDIA
Length:336
Mass (Da):37,140
Last modified:January 23, 2007 - v1
Checksum:i5E397201CAA94159
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009256 Genomic DNA. Translation: BAF39812.1.
RefSeqiWP_011743388.1. NC_008618.1.

Genome annotation databases

EnsemblBacteriaiBAF39812; BAF39812; BAD_1031.
GeneIDi4556415.
KEGGibad:BAD_1031.
PATRICi21101491. VBIBifAdo27973_1109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009256 Genomic DNA. Translation: BAF39812.1.
RefSeqiWP_011743388.1. NC_008618.1.

3D structure databases

ProteinModelPortaliA1A279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi367928.BAD_1031.

Proteomic databases

PRIDEiA1A279.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF39812; BAF39812; BAD_1031.
GeneIDi4556415.
KEGGibad:BAD_1031.
PATRICi21101491. VBIBifAdo27973_1109.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039995.
KOiK00791.
OMAiHTRNYAK.
OrthoDBiPOG091H00NK.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_BIFAA
AccessioniPrimary (citable) accession number: A1A279
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.