A1A1X6 (SYFA_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BAD_0928

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000006801
Sites
	Metal binding	273	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A1X6 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: F7E21EEEE4C666BC	FASTA	355	38,936
	10 20 30 40 50 60 MAEGAVFDAQ AVTDAVAEGI AKIENASTME ELKAIKTQYA GAESAMTQAS KAIGALPKDQ 70 80 90 100 110 120 KKDAGKLMGK LRADFGRAFG TKEKQVKAEE EARELAAETV DMTLPVNRKP LGARHPLPKL 130 140 150 160 170 180 MEDVEDFFIS MGWQISDGPE VETEWYDFDA LNFGPDHPAR QMQDTFYVKG NQAKDAAGFV 190 200 210 220 230 240 GSNMVLRTQT SSDQVRGLIT RGVPLYIACP GRVFRTDELD ATHTPVFHQV EALAVDKHLT 250 260 270 280 290 300 MADLKGVLDK LAVAMFGPEA KTRLRPSYFP FTEPSAELDL WFPDKKGGAG WLEWGGCGMV 310 320 330 340 350 NPNVLKSAGL DPEVYTGFAF GVGVERTLLL RHDINDMHDL VEGDVRFSEQ FVMGE

References

[1]

"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39709.1.
RefSeq	YP_909791.1. NC_008618.1.
3D structure databases
ProteinModelPortal	A1A1X6.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A1X6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4556529.
GenomeReviews	Gene locus BAD_0928 in contig AP009256_GR.
KEGG	bad:BAD_0928.
NMPDR	fig|1680.3.peg.1199.
PATRIC	21101283. VBIBifAdo27973_1005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BIFAA

Accession

Primary (citable) accession number: A1A1X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program