A1A1W2 (SYY_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	BAD_0914

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Tyrosine--tRNA ligase HAMAP MF_02006		PRO_1000088579
Regions
	Domain	368 – 425	58	S4 RNA-binding
	Motif	51 – 60	10	"HIGH" region HAMAP MF_02006
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	46	1	Tyrosine By similarity
	Binding site	181	1	Tyrosine By similarity
	Binding site	185	1	Tyrosine By similarity
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A1W2 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 4AB775F96957D7FA	FASTA	435	47,968
	10 20 30 40 50 60 MAHVIDFKEA GFDSVLDELE WRGLISQSTD RDRLAEALNG EPITYYCGFD PTAASLHIGN 70 80 90 100 110 120 LVQLINMRHL QAAGHHPIAL VGGATGLIGD PRQSGERTLN PKDVVAGWAD RLKKQIGGIL 130 140 150 160 170 180 ETEGSNPVRF VSNYDWTASM NVIDFLRDVG KNFRMGTMLA KDTVARRLNS EEGISFTEFS 190 200 210 220 230 240 YQVLQGNDFL HLFDEYHCVL EIGGSDQWGN LTSGLDLIHK VRGVDVNVFT SPIITDAQGK 250 260 270 280 290 300 KFGKSEGNAV WLDGTMLSPY KFYQFWFNRP DSEMENLLKA FTFLPKAEIE RLIEESKTNP 310 320 330 340 350 360 GAREAQRTLA WEVTSFVHGE EATRQAIEAA GALFGRGGDL ADIDESTLEA AIDGMKVDGE 370 380 390 400 410 420 FAKVAAGDRV AEAGMKAGLF KSISEARKTI KSGGVYLNNT RVEDEEQTLQ EGDFLHGRFV 430 LIRRGKKALG VVEQA

References

[1]

"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39695.1.
RefSeq	YP_909777.1. NC_008618.1.
3D structure databases
ProteinModelPortal	A1A1W2.
SMR	A1A1W2. Positions 15-326.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A1W2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4556733.
GenomeReviews	Gene locus BAD_0914 in contig AP009256_GR.
KEGG	bad:BAD_0914.
NMPDR	fig|1680.3.peg.1184.
PATRIC	21101249. VBIBifAdo27973_0988.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	TFYIGFD.
ProtClustDB	PRK05912.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024107. Tyr-tRNA-synth_bac_1. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BIFAA

Accession

Primary (citable) accession number: A1A1W2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program