Reviewed, UniProtKB/Swiss-Prot A1A1F4 (METE_BIFAA)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BAD_0756

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 768	768	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017225
Sites
	Metal binding	653	1	Zinc By similarity
	Metal binding	655	1	Zinc By similarity
	Metal binding	738	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1A1F4-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: C85989CB052F7FDE	FASTA	768	86,080
	10 20 30 40 50 60 MSALTSVSGF PRIGQNRELK KIIEAYWKGN TTLDEVRATA KELRAKHWKL QQAAGIDLIP 70 80 90 100 110 120 SNDFSYYDQM LDTAILLNVI PQRYQRLAFE NPEETLFAMG RGYQGEKGDV TALPMKKWFT 130 140 150 160 170 180 TNYHYLVPEI DSATDIKLNS TKPFDEFNEA KALGITTKPV LIGPYTFLKL ARNPQAEELD 190 200 210 220 230 240 YDKGLVNAVA AVYAEVVAKF AELGVQWIQI DEPYLVLDKE PGDVELFKSL YAKILPAREG 250 260 270 280 290 300 KVKVLLNTYF GHIADVYETV NLLGFDGIGL DLNEGKDENL AAVEKYGVAE KTTIFAGVIN 310 320 330 340 350 360 GRNIWRNNYA VSLGLVDALK QVTANVAVST ASSLLHVPFS TEGEDGLADD VRKHFAFAVQ 370 380 390 400 410 420 KLDELHEVAV LADASDDEKK ASAELAANQA LFDGTRVAAD PAVAKRIASL TDADFVRQPA 430 440 450 460 470 480 RAERQKEQRE ALNLPLLPTT TIGSFPQTKE VRAERAKLRK GEITKAEYDE FMKNQIDACI 490 500 510 520 530 540 KHQEEIGLDV LVHGEFERND MVEYFGQNLN GFLFTKNAWV QSYGTRCVKP PIVWGDVSRA 550 560 570 580 590 600 NPITVEWSAY AQSRTDHVMK GMLTGPVTIL NWSWPREDIT HEQQTQQLAL AIRDEVLDLE 610 620 630 640 650 660 KAGIKVIQID EAALREKLPL RKTDWHKKYL DWAIPAFRLV HSAVKPTTQI HTHMCYSEFN 670 680 690 700 710 720 DIIKDIDAMD ADVISFEASR GDLVVLDAIH DANFETEAGP GVYDIHSPRI PSEQEIEDRI 730 740 750 760 YEILKKMDVE KVWINPDCGL KTRGNAETWP SLENLVAAAK AVRAKLAK

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP009256 Genomic DNA. Translation: BAF39537.1.
RefSeq	YP_909619.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1A1F4.
Genome annotation databases
GeneID	4556409.
GenomeReviews	Gene locus BAD_0756 in contig AP009256_GR.
KEGG	bad:BAD_0756.
NMPDR	fig|1680.3.peg.1338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CSLLHTP.
Family and domain databases
HAMAP	MF_00172. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BIFAA

Accession

Primary (citable) accession number: A1A1F4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)