A1A184 (A1A184_BIFAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344
EC=6.3.5.2 HAMAP MF_00344

Alternative name(s):
GMP synthetase HAMAP MF_00344
Glutamine amidotransferase HAMAP MF_00344

Gene names

Name:	guaA HAMAP MF_00344 EMBL BAF39467.1
Ordered Locus Names:	BAD_0686

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 GMP-binding domain. HAMAP MF_00344 Contains 1 glutamine amidotransferase type-1 domain. HAMAP MF_00344

Ontologies

Keywords
Biological process	GMP biosynthesis HAMAP MF_00344 Purine biosynthesis
Domain	Glutamine amidotransferase HAMAP MF_00344
Ligand	ATP-binding HAMAP MF_00344 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00344
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Domain	5 – 195	191	Glutamine amidotransferase type-1 By similarity HAMAP MF_00344
	Domain	227 – 395	169	GMP-binding By similarity HAMAP MF_00344
	Nucleotide binding	223 – 229	7	ATP By similarity HAMAP MF_00344
Sites
	Active site	82	1	Nucleophile By similarity HAMAP MF_00344
	Active site	169	1	By similarity HAMAP MF_00344
	Active site	171	1	By similarity HAMAP MF_00344

Sequences

	Sequence		Length	Mass (Da)
	A1A184 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 6526BF89AE4AC4D1	FASTA	520	56,303
	10 20 30 40 50 60 MAAGPVLVVD FGAQYAQLIA RRVREANVYS ELVPHSMPVD EMLAKDPQAI ILSGGPASVF 70 80 90 100 110 120 EPGAPRVDKK LFDAGVPVLG ICYGFQAMAY ALGADVDKAA LGEYGKTETT IDKAEGLLDG 130 140 150 160 170 180 SPAEQSTWMS HGVAVKTAPE GFEVLAHTEG APVAAMQDES RKLYGVQWHP EVKHTPMGQK 190 200 210 220 230 240 LIENFLHKCA GLGDNWNASN IIEDQVKKIR EKVGDAQVIC GLSGGVDSAV AAALVHKAIG 250 260 270 280 290 300 EQLTCVFVDH GLLRKGEAEQ VKHDFVEATG IKLIAVDASE DFLTALKGVS EPEKKRKIIG 310 320 330 340 350 360 EKFIRTFEKA QRQVIEEAGA SGKEVKFLVQ GTLYPDVVES GGGDGAANIK SHHNVGGLPK 370 380 390 400 410 420 DIKFQLIEPL RTLFKDEVRA IGTELGLPDE IVWRQPFPGP GLGIRIIGEI TKERLDLLRE 430 440 450 460 470 480 ADAIAREELS KAGLDRDIWQ CPVVLLADVH SVGVQGDERT YGSPIVLRPV SSEDAMTADW 490 500 510 520 SRIPYDVLAT ISTRITNECR QINRVVLDCT SKPPATIEWE

References

[1]

"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39467.1.
RefSeq	YP_909549.1. NC_008618.1.
3D structure databases
ProteinModelPortal	A1A184.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A184.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4556142.
GenomeReviews	Gene locus BAD_0686 in contig AP009256_GR.
KEGG	bad:BAD_0686.
NMPDR	fig|1680.3.peg.1263.
PATRIC	21100733. VBIBifAdo27973_0742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0519.
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A1A184_BIFAA

Accession

Primary (citable) accession number: A1A184

Entry history

Integrated into UniProtKB/TrEMBL:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)