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Protein

Bifunctional protein FolD

Gene

folD

Organism
Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951NADPUniRule annotation
Binding sitei236 – 2361NADP; via amide nitrogenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi168 – 1703NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciBADO367928:GHPT-706-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Ordered Locus Names:BAD_0673
OrganismiBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Taxonomic identifieri367928 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
Proteomesi
  • UP000008702 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Bifunctional protein FolDPRO_0000305797Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi367928.BAD_0673.

Structurei

3D structure databases

ProteinModelPortaliA1A171.
SMRiA1A171. Positions 3-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.
OrthoDBiEOG6K6VBB.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

A1A171-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTRIDGKKV AARTKADLAK RVELLKARGI QPGLGTILVG SDPGSVKYVA
60 70 80 90 100
GKHADCAEIG VNSIKRELPE NATFDQVADA VRQLNADPAC TGYIVQLPLP
110 120 130 140 150
RGIDENAIID LIDPKKDADG MHPYNLGELV LHARGDITTP LPCTPRGVIE
160 170 180 190 200
LLRAYDIDLD GKEVCVLGRG ITIGRTIGLL LTRKAVNATV TLCHTGTKDV
210 220 230 240 250
RDHMRRADVI VAAMGVAGFV KPEDIKEGSI LVDVGVSRVF DEESGRYKVK
260 270 280 290
GDVDKACYEK AFAYTPNPGG VGPMTRAMLL QNVVEMAERQ L
Length:291
Mass (Da):31,278
Last modified:January 23, 2007 - v1
Checksum:iCFA4F8DF748EF34A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009256 Genomic DNA. Translation: BAF39454.1.
RefSeqiWP_011743094.1. NC_008618.1.

Genome annotation databases

EnsemblBacteriaiBAF39454; BAF39454; BAD_0673.
GeneIDi4556973.
KEGGibad:BAD_0673.
PATRICi21100703. VBIBifAdo27973_0729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009256 Genomic DNA. Translation: BAF39454.1.
RefSeqiWP_011743094.1. NC_008618.1.

3D structure databases

ProteinModelPortaliA1A171.
SMRiA1A171. Positions 3-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi367928.BAD_0673.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF39454; BAF39454; BAD_0673.
GeneIDi4556973.
KEGGibad:BAD_0673.
PATRICi21100703. VBIBifAdo27973_0729.

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
KOiK01491.
OMAiLMFNTIK.
OrthoDBiEOG6K6VBB.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciBADO367928:GHPT-706-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Bifidobacterium adolescentis complete genome sequence."
    Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K.
    Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Entry informationi

Entry nameiFOLD_BIFAA
AccessioniPrimary (citable) accession number: A1A171
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.