A1A165 (A1A165_BIFAA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685
EC=2.4.1.18 HAMAP MF_00685

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase HAMAP MF_00685
Alpha-(1->4)-glucan branching enzyme HAMAP MF_00685
Glycogen branching enzyme HAMAP MF_00685

Gene names

Name:	glgB HAMAP MF_00685 EMBL BAF39448.1
Ordered Locus Names:	BAD_0667

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	751 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685 SAAS SAAS006407
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685 SAAS SAAS006407
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis HAMAP MF_00685 SAAS SAAS006407 Glycogen metabolism HAMAP MF_00685
Molecular function	Glycosyltransferase HAMAP MF_00685 SAAS SAAS006407 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	432	1	Nucleophile By similarity HAMAP MF_00685
	Active site	485	1	Proton donor By similarity HAMAP MF_00685

Sequences

	Sequence		Length	Mass (Da)
	A1A165 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 5F02E4B328312A96	FASTA	751	84,570
	10 20 30 40 50 60 MATESKIKED SVAVPVAQGD LDAVSNGTFY NPHEVLGGHL GPDEHEDVVT IRVLRPLAKS 70 80 90 100 110 120 VTIITENART QAVHEHNGVF MALIPAIKTD DGFGVPDYRI STEYEDGSTV VSDDPYRYLP 130 140 150 160 170 180 TIGDLDMYLF GEGRHERLWE ALGARVLRYD DPLGSNDGVK GEQLAGTAFT VWAPNAHAVR 190 200 210 220 230 240 VVGDFNGWNG RTHAMRELGS SGVWELFIPG VGTGTIYKYE ILNANNEWVM KADPMERSHE 250 260 270 280 290 300 IPPRTGSIVV DSVHAWHDEN WMDQRAKTDP HNGPVSIYEV HASSWRKDVK NYRELADKLV 310 320 330 340 350 360 PYVQKEGFTH VEFMPLAEHP FSGSWGYQVT GYYAIDSRLG GPDDFKYLVE KMHEAGIGVI 370 380 390 400 410 420 MDWVPAHFPK DAFALGRFDG TPLYEDPDPT RGEHPDWGTY VFNFGRREVR NFLVANACFW 430 440 450 460 470 480 LDEYHIDALR VDAVSSMLYL DYSRKPGQWH PNIYGGRENL EAIDFLKEAT ATAYKNNPGI 490 500 510 520 530 540 MMIAEESTAY PGITAPTDAG GLGFGLKWNM GWMHDTLQYL HEEPINRKWH HNEITFSMVY 550 560 570 580 590 600 AYSEHYVLPI SHDEVVYGKG SLFGKMPGDD WQRYAGVRAL FAYQWAHPGK KLSFMGNEVA 610 620 630 640 650 660 QYGEWDHDGS VDWDVLNWPD HRGVQKLVAD LNTLYKASPA LWSQDFDPAG FQWLTSDDAD 670 680 690 700 710 720 HNTLSFVRIG KDGEQMVVVV NFSGEAWEDY QVPLTKGGKW TEVLTTDDKK YGGSDIHNGT 730 740 750 FKAVKGEYHS RDWSAKITVP ALGAVFLKPE L

References

[1]

"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39448.1.
RefSeq	YP_909530.1. NC_008618.1.
3D structure databases
ProteinModelPortal	A1A165.
ModBase	Search...
Protein-protein interaction databases
STRING	A1A165.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4557707.
GenomeReviews	Gene locus BAD_0667 in contig AP009256_GR.
KEGG	bad:BAD_0667.
NMPDR	fig|1680.3.peg.1430.
PATRIC	21100687. VBIBifAdo27973_0721.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1A165_BIFAA

Accession

Primary (citable) accession number: A1A165

Entry history

Integrated into UniProtKB/TrEMBL:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)