Reviewed, UniProtKB/Swiss-Prot A1A0T7 (ILVD_BIFAA)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BAD_0539

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000321592
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A1A0T7-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: D2B34592D3F4B9F4	FASTA	619	66,450
	10 20 30 40 50 60 MEMRSAKLMN GRVFAGARAL YRAAGVDGKD FGKPIIAIAN SFDEFLPGHV HLNKVGRLIS 70 80 90 100 110 120 DAIKEAGGIP REFNTMAVDD GIAMGHTGML YSLPSRDIIA DTVEYQVNAH CADALICIPN 130 140 150 160 170 180 CDKVVPGMLM AALRLNIPTV FVSGGPMEAG TTVLPDGTVK KNTDLIDVMY ASADDNLNEE 190 200 210 220 230 240 DLLAYEKTVC PTCGSCAGMF TANSMNCLTE AIGLALPGNG TILASHSYRK DLFKRAAEQV 250 260 270 280 290 300 VKIAKQYYDD DDDSVLPRSI ATKKAFENAM TMDVAMGGST NTVLHILAMA QSADVDFTLD 310 320 330 340 350 360 DIERISHTVP CICKASPSGE WEISDVHRAG GITGILGELD RAGKLHRDVH SIDYKSLEDK 370 380 390 400 410 420 LNDWDIMRDT CTEEAKQMYL AAPGHIVSPE PWTHTTLFDS LDRDRVNGAI HDIDHPAVTE 430 440 450 460 470 480 GGLAVLRGNL APDGCVVKTA GVPKEIWTFR GPALVVESQE QAIEVILNDT LKPGMALVIR 490 500 510 520 530 540 YEGPKGGPGM QEMLYPTSFV KGKGIGKQVA MLTDGRYSGG SSGLAIGHIA PEAANKGPIA 550 560 570 580 590 600 LIKNGDIINI DIPNRTVNVE LSDEELAQRR AELEAGDGYV AHRDRKVSQA LKAYAAFARS 610 ADKGATRDPE LIDKLSGLA

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39320.1.
RefSeq	YP_909402.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1A0T7.
Genome annotation databases
GeneID	4556215.
GenomeReviews	Gene locus BAD_0539 in contig AP009256_GR.
KEGG	bad:BAD_0539.
NMPDR	fig|1680.3.peg.800.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	ERISHTV.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BIFAA

Accession

Primary (citable) accession number: A1A0T7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)