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A1A0E0 (TRMB_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BAD_0392
OrganismBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]
Taxonomic identifier367928 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288125

Regions

Region273 – 2764Substrate binding By similarity

Sites

Active site1761 By similarity
Binding site1021S-adenosyl-L-methionine By similarity
Binding site1271S-adenosyl-L-methionine By similarity
Binding site1541S-adenosyl-L-methionine By similarity
Binding site1761S-adenosyl-L-methionine By similarity
Binding site1801Substrate By similarity
Binding site2121Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A1A0E0 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: 2DD5CC44BC18F9C6

FASTA29432,724
        10         20         30         40         50         60 
MQHDENTPTD GRKTEENAQN IQHVQSAAAD GRPALYGRKV LSFVRRSARL DARLQRAWDT 

        70         80         90        100        110        120 
YADTYLLNIN AGEGSLGVRE GFVFDQAYIR ETWGNTNPLI VEIGSGQGEN VVAAAAARPD 

       130        140        150        160        170        180 
VNFLALEVYD PGVAHTLLLA GKQNLKNLRI AQINAPELFK AAVDGSIAEV WTFFPDPWPK 

       190        200        210        220        230        240 
MRHHKRRIVQ PELAADIHRA LTDEGVWRIA TDIEDYALHV HEVMDELDGF HNDGALTVSL 

       250        260        270        280        290 
PVEHVGKGNA DEAAALRHAD FAESERFDGR VLTNFEKKGL AAGRVIHDFT YRRV 

« Hide

References

[1]"Bifidobacterium adolescentis complete genome sequence."
Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009256 Genomic DNA. Translation: BAF39173.1.
RefSeqYP_909255.1. NC_008618.1.

3D structure databases

ProteinModelPortalA1A0E0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING367928.BAD_0392.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF39173; BAF39173; BAD_0392.
GeneID4557443.
KEGGbad:BAD_0392.
PATRIC21100061. VBIBifAdo27973_0420.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAGWAPRFE.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycBADO367928:GHPT-412-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BIFAA
AccessionPrimary (citable) accession number: A1A0E0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways