Reviewed, UniProtKB/Swiss-Prot A0ZZZ1 (PROB_BIFAA)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BAD_0243

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Glutamate 5-kinase HAMAP MF_00456		PRO_1000081036
Regions
	Domain	285 – 359	75	PUA

Sequences

	Sequence		Length	Mass (Da)
	A0ZZZ1-1 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: B46D72B3D2EDB562	FASTA	377	39,224
	10 20 30 40 50 60 MNTPSQAQVR RAIAEAQTIV VKVGSSSLTE PSGHLDPQRL NALVAAIARV RLMGGRVVLV 70 80 90 100 110 120 SSGAIAAGFG TLGFDKRPTD VADQQACAAV GQGLLMAQYE AAFARYGLRV GQILITVSDT 130 140 150 160 170 180 IAPQQYRNVR RTLDRLLDLG AVPIINENDS LASNEIRFGD NDRLSALIAN IVVADALVLL 190 200 210 220 230 240 TDVDALYTAP PSEPGSKRIS YVPNVEDALA KVQVGGTGSN VGTGGMVTKM EAARVAAVSG 250 260 270 280 290 300 IPAVLTCASN AGPAMMGDPV GTAFAPINDR GSSRRLWIGF ASHPQGTLVA DSGAAKAVRG 310 320 330 340 350 360 GAASLLAAGV VEVKGDFAAG DAVWVDDEQG NHLAKGLVGF DSEEIPQMLG RNTAQLKRFL 370 GEEYAHPLVH RDNLVLV

References

[1]	"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF39024.1.
RefSeq	YP_909106.1.
3D structure databases
SMR	A0ZZZ1. Positions 15-376.
ModBase	Search...
Protein-protein interaction databases
STRING	A0ZZZ1.
Genome annotation databases
GeneID	4556587.
GenomeReviews	Gene locus BAD_0243 in contig AP009256_GR.
KEGG	bad:BAD_0243.
NMPDR	fig|1680.3.peg.506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HBG507643.
OMA	TDVDRLY.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. IPR015947. PUA-like. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BIFAA

Accession

Primary (citable) accession number: A0ZZZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)