A0ZZV4 (TRMD_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	BAD_0206

Organism

Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]

Taxonomic identifier

367928 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006453
Regions
	Region	182 – 187	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	157	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0ZZV4 [UniParc]. Last modified January 23, 2007. Version 1. Checksum: 2CD3E78B7D3087D6	FASTA	306	33,553
	10 20 30 40 50 60 MKIDIVSVFP EYFEVLNLSL LGKAQEKGLV EVTAHNLRDW THDVHHSVDD TPVGGGAGMV 70 80 90 100 110 120 MKPEVWSECL DELLHLEPTT VTCDSMVTCD STADADDTAS ADAAEPAESA TEIAANADTV 130 140 150 160 170 180 PATDRPVLIF PNPSAPLFTQ QDATELSHAN HLLFGCGRYE GYDARIPQYY RAQGVDVREY 190 200 210 220 230 240 SIGDYVLNGG EVAVSVMLEA ITRLLPGFMG NAASIVEESY TGENALLEHR QYTKPADWRG 250 260 270 280 290 300 IKVPDVLLSG DHAKVDRFRR DEALAKTNEL RPDLIEALDC SKLDKADRKT LMALGWEVSG AHPRQR

References

[1]

"Bifidobacterium adolescentis complete genome sequence." Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009256 Genomic DNA. Translation: BAF38987.1.
RefSeq	YP_909069.1. NC_008618.1.
3D structure databases
ProteinModelPortal	A0ZZV4.
ModBase	Search...
Protein-protein interaction databases
STRING	A0ZZV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4557660.
GenomeReviews	Gene locus BAD_0206 in contig AP009256_GR.
KEGG	bad:BAD_0206.
NMPDR	fig|1680.3.peg.470.
PATRIC	21099641. VBIBifAdo27973_0222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	RYLYDRE.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMD_BIFAA

Accession

Primary (citable) accession number: A0ZZV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program