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A0ZZT4 (DDL_BIFAA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:BAD_0186
OrganismBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) [Complete proteome] [HAMAP]
Taxonomic identifier367928 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length378 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 378378D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030432

Regions

Domain149 – 374226ATP-grasp
Nucleotide binding189 – 24759ATP By similarity

Sites

Metal binding3281Magnesium or manganese 1 By similarity
Metal binding3411Magnesium or manganese 1 By similarity
Metal binding3411Magnesium or manganese 2 By similarity
Metal binding3431Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A0ZZT4 [UniParc].

Last modified January 23, 2007. Version 1.
Checksum: BBDC4977384B8C56

FASTA37840,528
        10         20         30         40         50         60 
MAKKRIVVMY GGKADEHSIS CISAASALRA LDTDKFEAIP VGITKDGKWI VNGENPLGWS 

        70         80         90        100        110        120 
LDEGLPTVEK TPGAKDVVLE VALGQDGFFA REDDGTMTPF GHVDAVFPVL HGPYGEDGTI 

       130        140        150        160        170        180 
QGLFEMMGVP YVGCGVLASA ACMDKHYTKV LLAAAGIPVA PGITLDARSF DKASEFKTDA 

       190        200        210        220        230        240 
DAIMAQVSEA GLQYPLFVKP SRAGSSFGVT KVEHEGDAAE LAAAVYEASR HDWRILVEQG 

       250        260        270        280        290        300 
IDAREIECAV LCPKAGEAPQ ASWPGEIVLD KRAEGDDQFY DFDSKYMDAA ASHVEVPANL 

       310        320        330        340        350        360 
PEETLNLVRE TAKKAFVAVD GAGLSRVDTF VTKDGKVMVN EINTMPGFTS ISMYPKAWEA 

       370 
TGVRYTDLIT KLIEGVLR 

« Hide

References

[1]"Bifidobacterium adolescentis complete genome sequence."
Suzuki T., Tsuda Y., Kanou N., Inoue T., Kumazaki K., Nagano S., Hirai S., Tanaka K., Watanabe K.
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15703 / DSM 20083 / NCTC 11814 / E194a.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009256 Genomic DNA. Translation: BAF38967.1.
RefSeqYP_909049.1. NC_008618.1.

3D structure databases

ProteinModelPortalA0ZZT4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING367928.BAD_0186.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF38967; BAF38967; BAD_0186.
GeneID4556245.
KEGGbad:BAD_0186.
PATRIC21099595. VBIBifAdo27973_0199.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBADO367928:GHPT-194-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BIFAA
AccessionPrimary (citable) accession number: A0ZZT4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways