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Protein

Sucrose phosphorylase

Gene

sucP

Organism
Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible phosphorolysis of sucrose into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose (PubMed:14740189, PubMed:20691225). Is involved in sucrose degradation. Also displays transglucosylation activity in vitro, by transferring the glucosyl moiety of Glc1P to a broad range of monomeric sugars, such as D- and L-arabinose, D- and L-arabitol, and xylitol (PubMed:14740189).2 Publications

Catalytic activityi

Sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate.2 Publications

Kineticsi

kcat is 207 sec(-1) for the phosphorolysis of sucrose (at 58 degrees Celsius and pH 6.5).1 Publication
  1. KM=6.8 mM for sucrose (at 58 degrees Celsius and pH 6.5)1 Publication

    pH dependencei

    Optimum pH is 6.0-6.5 for the phosphorolysis of sucrose.2 Publications

    Temperature dependencei

    Optimum temperature is 48 degrees Celsius for the phosphorolysis of sucrose (PubMed:14740189). The His-tagged enzyme shows an optimum temperature of 58 degrees Celsius (PubMed:20691225). The immobilization of the enzyme on Sepabeads EC-HFA increases thermostability, and optimum temperature is shifted to 65 degrees Celsius (PubMed:20691225).2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei50SubstrateCombined sources1 Publication1
    Binding sitei88SubstrateCombined sources1 Publication1
    Active sitei192Nucleophile1 Publication1 Publication1
    Active sitei232Proton donor2 Publications1
    Binding sitei232SubstrateCombined sources1 Publication1
    Binding sitei399SubstrateCombined sources1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    Biological processCarbohydrate metabolism

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sucrose phosphorylase3 Publications (EC:2.4.1.72 Publications)
    Short name:
    SP1 Publication
    Short name:
    SPase1 Publication
    Gene namesi
    Name:sucP1 Publication
    Synonyms:splImported
    Ordered Locus Names:BAD_0078Imported
    OrganismiBifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)
    Taxonomic identifieri367928 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaBifidobacterialesBifidobacteriaceaeBifidobacterium
    Proteomesi
    • UP000008702 Componenti: Chromosome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi232E → Q: Loss of catalytic activity. 1 Publication1
    Mutagenesisi345Q → F: Decreases affinity for sucrose. Highly improves in vitro activity towards aromatic acceptors, allowing efficient glucosylation of resveratrol, (+)-catechin and (-)-epicatechin. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004424351 – 504Sucrose phosphorylaseAdd BLAST504

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi367928.BAD_0078.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1R7AX-ray1.77A/B1-504[»]
    2GDUX-ray2.10A/B1-504[»]
    2GDVX-ray2.00A/B1-504[»]
    5C8BX-ray2.70B1-504[»]
    ProteinModelPortaliA0ZZH6.
    SMRiA0ZZH6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni190 – 192Substrate bindingCombined sources1 Publication3
    Regioni289 – 290Substrate bindingCombined sources1 Publication2
    Regioni342 – 345Substrate bindingCombined sources1 Publication4

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107RK0. Bacteria.
    COG0366. LUCA.
    HOGENOMiHOG000220752.
    KOiK00690.
    OMAiNFTASHD.
    OrthoDBiPOG091H0HTE.

    Family and domain databases

    CDDicd11355. AmyAc_Sucrose_phosphorylase. 1 hit.
    InterProiView protein in InterPro
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR022527. SP_GftA.
    IPR015325. Suc_Porlyase_C.
    IPR016377. Sucrose_phosphorylase-rel.
    PfamiView protein in Pfam
    PF00128. Alpha-amylase. 1 hit.
    PF09244. DUF1964. 1 hit.
    PIRSFiPIRSF003059. Sucrose_phosphorylase. 1 hit.
    SMARTiView protein in SMART
    SM00642. Aamy. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03852. sucrose_gtfA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0ZZH6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKNKVQLITY ADRLGDGTIK SMTDILRTRF DGVYDGVHIL PFFTPFDGAD
    60 70 80 90 100
    AGFDPIDHTK VDERLGSWDD VAELSKTHNI MVDAIVNHMS WESKQFQDVL
    110 120 130 140 150
    AKGEESEYYP MFLTMSSVFP NGATEEDLAG IYRPRPGLPF THYKFAGKTR
    160 170 180 190 200
    LVWVSFTPQQ VDIDTDSDKG WEYLMSIFDQ MAASHVSYIR LDAVGYGAKE
    210 220 230 240 250
    AGTSCFMTPK TFKLISRLRE EGVKRGLEIL IEVHSYYKKQ VEIASKVDRV
    260 270 280 290 300
    YDFALPPLLL HALSTGHVEP VAHWTDIRPN NAVTVLDTHD GIGVIDIGSD
    310 320 330 340 350
    QLDRSLKGLV PDEDVDNLVN TIHANTHGES QAATGAAASN LDLYQVNSTY
    360 370 380 390 400
    YSALGCNDQH YIAARAVQFF LPGVPQVYYV GALAGKNDME LLRKTNNGRD
    410 420 430 440 450
    INRHYYSTAE IDENLKRPVV KALNALAKFR NELDAFDGTF SYTTDDDTSI
    460 470 480 490 500
    SFTWRGETSQ ATLTFEPKRG LGVDNTTPVA MLEWEDSAGD HRSDDLIANP

    PVVA
    Length:504
    Mass (Da):56,190
    Last modified:January 23, 2007 - v1
    Checksum:i10705FE3182E5754
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF543301 Genomic DNA. Translation: AAO33821.1.
    AP009256 Genomic DNA. Translation: BAF38859.1.
    RefSeqiWP_011742626.1. NC_008618.1.

    Genome annotation databases

    EnsemblBacteriaiBAF38859; BAF38859; BAD_0078.
    GeneIDi4556453.
    KEGGibad:BAD_0078.

    Similar proteinsi

    Entry informationi

    Entry nameiSUCP_BIFAA
    AccessioniPrimary (citable) accession number: A0ZZH6
    Secondary accession number(s): Q84HQ2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2017
    Last sequence update: January 23, 2007
    Last modified: November 22, 2017
    This is version 71 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families