Photosystem I P700 chlorophyll a apoprotein A2 - Thalassiosira pseudonana (Marine diatom)

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A0T0M9 (PSAB_THAPS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 38. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Photosystem I P700 chlorophyll a apoprotein A2
EC=1.97.1.12

Alternative name(s):
PSI-B
PsaB

Gene names

Name:

psaB

Encoded on

Plastid; Chloroplast

Organism

Thalassiosira pseudonana (Marine diatom) (Cyclotella nana) [Reference proteome]

Taxonomic identifier

35128 [NCBI]

Taxonomic lineage

Eukaryota › Stramenopiles › Bacillariophyta › Coscinodiscophyceae › Thalassiosirophycidae › Thalassiosirales › Thalassiosiraceae › Thalassiosira

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6 By similarity. HAMAP-Rule MF_00482
Catalytic activity	Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin. HAMAP-Rule MF_00482
Cofactor	P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center By similarity.
Subunit structure	The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein HAMAP-Rule MF_00482.
Sequence similarities	Belongs to the PsaA/PsaB family.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Membrane Photosystem I Plastid Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	4Fe-4S Chlorophyll Chromophore Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW photosystem I Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW chlorophyll binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 733

733

Photosystem I P700 chlorophyll a apoprotein A2 HAMAP-Rule MF_00482

PRO_0000277143

Regions

Transmembrane

46 – 69

Helical; Name=I; Potential

Transmembrane

134 – 157

Helical; Name=II; Potential

Transmembrane

174 – 198

Helical; Name=III; Potential

Transmembrane

272 – 290

Helical; Name=IV; Potential

Transmembrane

329 – 352

Helical; Name=V; Potential

Transmembrane

368 – 394

Helical; Name=VI; Potential

Transmembrane

416 – 438

Helical; Name=VII; Potential

Transmembrane

516 – 534

Helical; Name=VIII; Potential

Transmembrane

574 – 595

Helical; Name=IX; Potential

Transmembrane

642 – 664

Helical; Name=X; Potential

Transmembrane

706 – 726

Helical; Name=XI; Potential

Sites

Metal binding

558

Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity

Metal binding

567

Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity

Metal binding

653

Magnesium (chlorophyll-a B1 axial ligand; P700 special pair) By similarity

Metal binding

661

Magnesium (chlorophyll-a B3 axial ligand) By similarity

Binding site

669

Chlorophyll-a B3 By similarity

Binding site

670

Phylloquinone B By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A0T0M9 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: F5C6FCBE674FB914
Show »

FASTA

733

82,089

        10         20         30         40         50         60 
MATKFPKFSQ ALAQDPATRR IWYGIATAHD LEAHDGMTEE NLYQKIFASH FGHLAIIFLW 

        70         80         90        100        110        120 
TSGNLFHVAW QGNFEKWVSN PLKTRPIAHS IWDPHFGESA LKAFSKGNTY PVNITFSGLY 

       130        140        150        160        170        180 
QWWYTIGFRT NQELYKGSIG LLLLASVLLI AGWLHLQPKF RPSLSWFKNN ESRLNHHLSG 

       190        200        210        220        230        240 
LLGFSSLAWT GHLVHVAIPA SRGVHVGWDN FLTTPPHPAG LTPFFTGNWT VYAENPDSAT 

       250        260        270        280        290        300 
HVFNTSEGSG TAILTFLGGF HPQTQSLWLS DMAHHHLAIA VVFIVAGHMY RTNFGIGHNM 

       310        320        330        340        350        360 
KEILDAHRPP GGRLGAGHVG LFETITNSLH MQLGLALACL GVATSLTAQH MYALTPYAYL 

       370        380        390        400        410        420 
SKDFTTEAAL YTHHQYIAGF LMVGAFAHGA IFFVRDYDPE LNKNNVLARM LEHKEAIISH 

       430        440        450        460        470        480 
LSWASLFLGF HTLGLYIHND TVVAFGQPEK QILFEPLFAE YIQAASGKAV YQFNVLLASS 

       490        500        510        520        530        540 
TSPATAAGNQ VWLPGWLEAI NNPKTDLFLK IGPGDFLVHH AIALGLHVTA LILVKGALDA 

       550        560        570        580        590        600 
RGSKLMPDKK DFGYSFPCDG PGRGGTCDIS AWDAFYLAMF WMLNTIGWVT FYWHWKHMTI 

       610        620        630        640        650        660 
WGGNPGQFDE SSNYIMGWLR DYLWLNSSPL INGYNPFGMN NLSVWSWMFL FGHLIWATGF 

       670        680        690        700        710        720 
MFLISWRGYW QELIETLVWA HERTPLANLI RWRDKPVALS IVQARLVGLV HFSVGYILTY 

       730 
AAFVIASTSG KFA

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References

[1]

"Chloroplast genomes of the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana: comparison with other plastid genomes of the red lineage."
Oudot-Le Secq M.-P., Grimwood J., Shapiro H., Armbrust E.V., Bowler C., Green B.R.
Mol. Genet. Genomics 277:427-439(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCMP1335.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF067921 Genomic DNA. Translation: ABK20714.1.
RefSeq	YP_874491.1. NC_008589.1.
3D structure databases
ProteinModelPortal	A0T0M9.
SMR	A0T0M9. Positions 2-732.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	Thapsdraft1611; Thapsdraft1611; Thapsdraft1611.
GeneID	4524814.
Phylogenomic databases
eggNOG	NOG04867.
HOGENOM	HOG000276868.
ProtClustDB	CHL00054.
Family and domain databases
Gene3D	1.20.1130.10. 1 hit.
HAMAP	MF_00482. PSI_PsaB.
InterPro	IPR001280. PSI_PsaA/B. IPR020586. PSI_PsaA/B_CS. IPR006244. PSI_PsaB. [Graphical view]
Pfam	PF00223. PsaA_PsaB. 1 hit. [Graphical view]
PIRSF	PIRSF002905. PSI_A. 1 hit.
PRINTS	PR00257. PHOTSYSPSAAB.
TIGRFAMs	TIGR01336. psaB. 1 hit.
PROSITE	PS00419. PHOTOSYSTEM_I_PSAAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSAB_THAPS

Accession

Primary (citable) accession number: A0T0M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	January 9, 2007
Last modified:	April 3, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents