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A0T0M8 (PSAA_THAPS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Photosystem I P700 chlorophyll a apoprotein A1

EC=1.97.1.12
Alternative name(s):
PSI-A
PsaA
Gene names
Name:psaA
Encoded onPlastid; Chloroplast
OrganismThalassiosira pseudonana (Marine diatom) (Cyclotella nana) [Reference proteome]
Taxonomic identifier35128 [NCBI]
Taxonomic lineageEukaryotaStramenopilesBacillariophytaCoscinodiscophyceaeThalassiosirophycidaeThalassiosiralesThalassiosiraceaeThalassiosira

Protein attributes

Sequence length752 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6. HAMAP-Rule MF_00458

Catalytic activity

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin. HAMAP-Rule MF_00458

Cofactor

P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center By similarity.

Subunit structure

The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits By similarity.

Subcellular location

Plastidchloroplast thylakoid membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00458.

Sequence similarities

Belongs to the PsaA/PsaB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 752752Photosystem I P700 chlorophyll a apoprotein A1 HAMAP-Rule MF_00458
PRO_0000275972

Regions

Transmembrane73 – 9624Helical; Name=I; Potential
Transmembrane159 – 18224Helical; Name=II; Potential
Transmembrane198 – 22225Helical; Name=III; Potential
Transmembrane294 – 31219Helical; Name=IV; Potential
Transmembrane349 – 37224Helical; Name=V; Potential
Transmembrane388 – 41427Helical; Name=VI; Potential
Transmembrane436 – 45823Helical; Name=VII; Potential
Transmembrane533 – 55119Helical; Name=VIII; Potential
Transmembrane591 – 61222Helical; Name=IX; Potential
Transmembrane666 – 68823Helical; Name=X; Potential
Transmembrane726 – 74621Helical; Name=XI; Potential

Sites

Metal binding5751Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Metal binding5841Iron-sulfur (4Fe-4S); shared with dimeric partner By similarity
Metal binding6771Magnesium (chlorophyll-a' A1 axial ligand; P700 special pair) By similarity
Metal binding6851Magnesium (chlorophyll-a A3 axial ligand) By similarity
Binding site6931Chlorophyll-a A3 By similarity
Binding site6941Phylloquinone A By similarity

Sequences

Sequence LengthMass (Da)Tools
A0T0M8 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: E6DDF50B4BF1415E

FASTA75283,641
        10         20         30         40         50         60 
MAISSTERRS KNVQVFVEKD AVETSFAKWA QPGHFSRTLA KGPKTTTWIW NLHADAHDFD 

        70         80         90        100        110        120 
SQTSSLEEVS RKIFSAHFGQ LAIIFLWISG MHFHGAYFSN YSAWLTDPIS IKQSSQVVWP 

       130        140        150        160        170        180 
IVGQEILNAD VGGNFQGIQT TSGWFQMWRA EGITSEVELY WTAIGGLAMS AIMLFAGWFH 

       190        200        210        220        230        240 
YHKAAPKLEW FQNAESMMNH HLAGLLGLGC LSWSGHQIHI ALPINKLLDA GVSPQEIPLP 

       250        260        270        280        290        300 
HEFLINRDLM AQLYPSFSKG LAPFFGGNWG EYSDFLTFKG GLNPVTGGLW LSDIAHHHLA 

       310        320        330        340        350        360 
LSVLFIIAGH MYRTNWGIGH NMKEILEAHK GPFTGEGHKG LYEILTTSWH AQLAINLAMM 

       370        380        390        400        410        420 
GSLSIIVAHH MYAMPPYPYL ATDYATQLSL FTHHMWIGGF CVVGGAAHGA IFMVRDYTPA 

       430        440        450        460        470        480 
NNYNNLLDRV LRHRDAIISH LNWVCIFLGC HAFGFYIHND TMRALGRPQD MFSDKAIQLQ 

       490        500        510        520        530        540 
PIFAQWIQNI HLLAPGTTAP NALATTSYAF GGDVIEVGGK IAMMPIKLGT ADFMVHHIHA 

       550        560        570        580        590        600 
FTIHVTVLIL LKGVLYARSS KLIPDKANLG FRFPCDGPGR GGTCQSSSWD HVFLGLFWMY 

       610        620        630        640        650        660 
NSISVVIFHF SWKMQSDVWG TITPDGAISH ITGGNFAQSS ITINGWLRDF LWSQASQVIQ 

       670        680        690        700        710        720 
SYGSASSAYG LIFLGAHFIW AFSLMFLFSG RGYWQELIES IVWAHNKLNF APTIQPRALS 

       730        740        750 
ITQGRAVGLA HYLLGGIGTT WAFFLARAIS IT 

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References

[1]"Chloroplast genomes of the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana: comparison with other plastid genomes of the red lineage."
Oudot-Le Secq M.-P., Grimwood J., Shapiro H., Armbrust E.V., Bowler C., Green B.R.
Mol. Genet. Genomics 277:427-439(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCMP1335.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EF067921 Genomic DNA. Translation: ABK20713.1.
RefSeqYP_874490.1. NC_008589.1.

3D structure databases

ProteinModelPortalA0T0M8.
SMRA0T0M8. Positions 14-751.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4524813.

Family and domain databases

Gene3D1.20.1130.10. 1 hit.
HAMAPMF_00458. PSI_PsaA.
InterProIPR006243. PSI_PsaA.
IPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
[Graphical view]
PfamPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFPIRSF002905. PSI_A. 1 hit.
PRINTSPR00257. PHOTSYSPSAAB.
SUPFAMSSF81558. SSF81558. 1 hit.
TIGRFAMsTIGR01335. psaA. 1 hit.
PROSITEPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSAA_THAPS
AccessionPrimary (citable) accession number: A0T0M8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families