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Protein

Photosystem I P700 chlorophyll a apoprotein A1

Gene

psaA

Organism
Phaeodactylum tricornutum (strain CCAP 1055/1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

PsaA and PsaB bind P700, the primary electron donor of photosystem I (PSI), as well as the electron acceptors A0, A1 and FX. PSI is a plastocyanin/cytochrome c6-ferredoxin oxidoreductase, converting photonic excitation into a charge separation, which transfers an electron from the donor P700 chlorophyll pair to the spectroscopically characterized acceptors A0, A1, FX, FA and FB in turn. Oxidized P700 is reduced on the lumenal side of the thylakoid membrane by plastocyanin or cytochrome c6.

Catalytic activityi

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin.UniRule annotation

Cofactori

Note: P700 is a chlorophyll a/chlorophyll a' dimer, A0 is one or more chlorophyll a, A1 is one or both phylloquinones and FX is a shared 4Fe-4S iron-sulfur center.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi575 – 5751Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi584 – 5841Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation
Metal bindingi677 – 6771Magnesium (chlorophyll-a' A1 axial ligand; P700 special pair)UniRule annotation
Metal bindingi685 – 6851Magnesium (chlorophyll-a A3 axial ligand)UniRule annotation
Binding sitei693 – 6931Chlorophyll-a A3UniRule annotation
Binding sitei694 – 6941Phylloquinone AUniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. chlorophyll binding Source: UniProtKB-KW
  3. electron carrier activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. oxidoreductase activity Source: UniProtKB-KW

GO - Biological processi

  1. photosynthesis Source: UniProtKB-HAMAP
  2. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

4Fe-4S, Chlorophyll, Chromophore, Iron, Iron-sulfur, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem I P700 chlorophyll a apoprotein A1UniRule annotation (EC:1.97.1.12UniRule annotation)
Alternative name(s):
PSI-AUniRule annotation
PsaAUniRule annotation
Gene namesi
Name:psaAUniRule annotation
Encoded oniPlastid; Chloroplast
OrganismiPhaeodactylum tricornutum (strain CCAP 1055/1)
Taxonomic identifieri556484 [NCBI]
Taxonomic lineageiEukaryotaStramenopilesBacillariophytaBacillariophyceaeBacillariophycidaeNaviculalesPhaeodactylaceaePhaeodactylum
ProteomesiUP000000759: Chloroplast

Subcellular locationi

Plastidchloroplast thylakoid membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei73 – 9624Helical; Name=IUniRule annotationAdd
BLAST
Transmembranei159 – 18224Helical; Name=IIUniRule annotationAdd
BLAST
Transmembranei198 – 22225Helical; Name=IIIUniRule annotationAdd
BLAST
Transmembranei294 – 31219Helical; Name=IVUniRule annotationAdd
BLAST
Transmembranei349 – 37224Helical; Name=VUniRule annotationAdd
BLAST
Transmembranei388 – 41427Helical; Name=VIUniRule annotationAdd
BLAST
Transmembranei436 – 45823Helical; Name=VIIUniRule annotationAdd
BLAST
Transmembranei533 – 55119Helical; Name=VIIIUniRule annotationAdd
BLAST
Transmembranei591 – 61222Helical; Name=IXUniRule annotationAdd
BLAST
Transmembranei666 – 68823Helical; Name=XUniRule annotationAdd
BLAST
Transmembranei726 – 74621Helical; Name=XIUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. photosystem I Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Photosystem I P700 chlorophyll a apoprotein A1PRO_0000275971Add
BLAST

Interactioni

Subunit structurei

The PsaA/B heterodimer binds the P700 chlorophyll special pair and subsequent electron acceptors. PSI consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation. The eukaryotic PSI reaction center is composed of at least 11 subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0T0L9.
SMRiA0T0L9. Positions 14-751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PsaA/PsaB family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiA0T0L9.

Family and domain databases

Gene3Di1.20.1130.10. 1 hit.
HAMAPiMF_00458. PSI_PsaA.
InterProiIPR006243. PSI_PsaA.
IPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
[Graphical view]
PfamiPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFiPIRSF002905. PSI_A. 1 hit.
PRINTSiPR00257. PHOTSYSPSAAB.
SUPFAMiSSF81558. SSF81558. 1 hit.
TIGRFAMsiTIGR01335. psaA. 1 hit.
PROSITEiPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0T0L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISSTDRRS KNVQIFVEKD AVETSFAKWA QPGHFSRTLA KGPKTTTWIW
60 70 80 90 100
NLHADAHDFD SQTSSLEEVS RKIFSAHFGQ LSVIFLWISG MHFHGAYFSN
110 120 130 140 150
YSAWLTDPVN IKQSSQVVWP IVGQEILNGD VGGNFQGIQT TSGWFQMWRA
160 170 180 190 200
EGITSEVELY WIAIGGLAMS AIMLFAGWFH YHKAAPKLEW FQNAESMMNH
210 220 230 240 250
HLAGLLGLGC LSWSGHQIHV ALPINKLLDA GVAPQEIPLP HEFLINRELM
260 270 280 290 300
SQLYPSFSKG LAPFFSGHWG EYSDFLTFKG GLNPVTGGLW LSDIAHHHLA
310 320 330 340 350
LAVLFIFAGH MYRTNWGIGH SMKEILEAHK GPFTGEGHKG LYEILTTSWH
360 370 380 390 400
AQLAINLAMM GSLSIIVAHH MYAMPPYPYI ATDYATQLSL FTHHMWIGGF
410 420 430 440 450
CVTGGAAHAA IFMVRDYTPA NNYNNLLDRV LRHRDSIIAH LNWVCIFLGC
460 470 480 490 500
HAFGFYIHND TMRALGRPQD MFSDKAIQLQ PIFAQWIQNI HLLAPGTTAP
510 520 530 540 550
NALATTSYAF GGEVVEVGGK IAMMPIQLGT ADFMVHHIHA FTIHVTVLIL
560 570 580 590 600
LKGVLYARSS KLIPDKANLG FRFPCDGPGR GGTCQSSSWD HVFLGLFWMY
610 620 630 640 650
NSISVVIFHF SWKMQSDVWG TISPDGSISH ITGGNFAKGA ITINGWLRDF
660 670 680 690 700
LWSQASQVIQ SYGSAASAYG LIFLGAHFIW AFSLMFLFSG RGYWQELIES
710 720 730 740 750
IVWAHNKLNF APAIQPRALS ITQGRAVGLA HYLLGGIGTT WAFFLARAVS

IS
Length:752
Mass (Da):83,596
Last modified:January 9, 2007 - v1
Checksum:iA2FEB64333F4C252
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF067920 Genomic DNA. Translation: ABK20582.1.
RefSeqiYP_874359.1. NC_008588.1.

Genome annotation databases

GeneIDi4524636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EF067920 Genomic DNA. Translation: ABK20582.1.
RefSeqiYP_874359.1. NC_008588.1.

3D structure databases

ProteinModelPortaliA0T0L9.
SMRiA0T0L9. Positions 14-751.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4524636.

Phylogenomic databases

InParanoidiA0T0L9.

Family and domain databases

Gene3Di1.20.1130.10. 1 hit.
HAMAPiMF_00458. PSI_PsaA.
InterProiIPR006243. PSI_PsaA.
IPR001280. PSI_PsaA/B.
IPR020586. PSI_PsaA/B_CS.
[Graphical view]
PfamiPF00223. PsaA_PsaB. 1 hit.
[Graphical view]
PIRSFiPIRSF002905. PSI_A. 1 hit.
PRINTSiPR00257. PHOTSYSPSAAB.
SUPFAMiSSF81558. SSF81558. 1 hit.
TIGRFAMsiTIGR01335. psaA. 1 hit.
PROSITEiPS00419. PHOTOSYSTEM_I_PSAAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Chloroplast genomes of the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana: comparison with other plastid genomes of the red lineage."
    Oudot-Le Secq M.-P., Grimwood J., Shapiro H., Armbrust E.V., Bowler C., Green B.R.
    Mol. Genet. Genomics 277:427-439(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCAP 1055/1.

Entry informationi

Entry nameiPSAA_PHATC
AccessioniPrimary (citable) accession number: A0T0L9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: January 9, 2007
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.