Reviewed, UniProtKB/Swiss-Prot A0RYM0 (DP2L_CENSY)

Names and origin

Protein names

Recommended name:
    DNA polymerase II large subunit
      Short name=Pol II
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	CENSYa_1827

Organism

Cenarchaeum symbiosum [Complete proteome] [HAMAP]

Taxonomic identifier

46770 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Cenarchaeales › Cenarchaeaceae › Cenarchaeum

Protein attributes

Sequence length	1112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process, exonucleolytic Inferred from electronic annotation. Source: HAMAP DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1112	1112	DNA polymerase II large subunit HAMAP MF_00324		PRO_0000294676

Sequences

	Sequence		Length	Mass (Da)
	A0RYM0-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: BA05392302C4ECF4	FASTA	1,112	121,039
	10 20 30 40 50 60 MSGGDVEEYL RGADMPEEYR RYYSSLSGGT YDIFEKAASA KSNLADSSGM VEPKIAVDLS 70 80 90 100 110 120 DRVAKMHDLD IAGPLRELLA TKGKELAALM LAKEIMDGKY LPEADIEKRI DSAVRVGLAV 130 140 150 160 170 180 VTEGVTIAPL QGIADVITKK NKDGSEYLSV SIAGPMRSAG GTESAVTMLI ADYVRRQAGL 190 200 210 220 230 240 AEYQADSLDD ETGRFIEELR IYEREVGSFQ FHVLDEDIRA VISHLPVELD GVDTDPYEVV 250 260 270 280 290 300 NHKGMSRIQT DRVRGGALRV LNDGLIGRSK KLLKRIELYG LDGWEWLAEL KGAVQTGENK 310 320 330 340 350 360 EDAAAKRMRE VITGRSVLSM PNRLGGFRLR YGRACNTGYT SVGFHPAVAE ILDHTIAVGT 370 380 390 400 410 420 QVKIDIPGKG ATVAFVDTIE APTVRLAGGD VVKIRDVAHG IELKGSIERI LHLGDMLISF 430 440 450 460 470 480 GDFLENNAQL VPSGYVEEIW KMDMEAAGAA QGSPSSADEA VRISRELGVP LHPRYLYYWD 490 500 510 520 530 540 QISHEELAML LSPLDKGDAI SYPAACKPVL EKLGVPHKAG PEGPVLEGDE ARIFRELILD 550 560 570 580 590 600 NPPGPDASAP VPELISRSSG ITIRDKFSTS IGVRIGRPEK AAPRQMRPPT HCLFPVGGTG 610 620 630 640 650 660 GPTNNLLKSA ARPGFSADIL SRRCPGCGEP SISIRCWACG ERTAVERTCM QCGTDVDGEE 670 680 690 700 710 720 CERCGRPGLA HSRVEFPLKK MLVSAQEKTG VRAHDPLKGV KELAHQDRIA EPLEKGLIRQ 730 740 750 760 770 780 SRSLTVFKDG TVRFDATNSP MTHFKPSWIG TSAEKLRELG YETDVDGKKL EGPDQLVELR 790 800 810 820 830 840 MQDIVIPLEG AKYLVSACGY IDAELDKLYG APPFYKVPDL GGLIGHLVVG LAPHTSVGVA 850 860 870 880 890 900 ARIIGYTETH VCFGTPNWHS AKRRDADGDA DSIILLMDAL LNFSRHYLSD RIGGLMDAPL 910 920 930 940 950 960 LIQPLVLPHE SQSQAHNIEV VKRLPPGFYE AAAARKKASE VGCVEIVKSR LETAGQFGGY 970 980 990 1000 1010 1020 HFTHGTSSLT TSRPRSAYST LGSMLDKLDL QIRNANLIAA VDAAEIISNV ISTHLVPDIM 1030 1040 1050 1060 1070 1080 GNLRAYARQN FRCTACGKSY RRIPLAQRCS CGNGLIQTIT RASVEKYLKL AKRLVNEYDV 1090 1100 1110 GAYQRGRIHA LSDEIELVFG KGGGDQALLT DF

References

[1]

"Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum." Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y., Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F. Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006) [PubMed: 17114289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DP000238 Genomic DNA. Translation: ABK78437.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus CENSYa_1827 in contig DP000238_GR.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	2.7.7.7. 281607.
Family and domain databases
HAMAP	MF_00324. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00354. polC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_CENSY

Accession

Primary (citable) accession number: A0RYM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	January 9, 2007
Last modified:	January 20, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)