A0RX10 (SYA_CENSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CENSYa_1254

Organism

Cenarchaeum symbiosum (strain A)

Taxonomic identifier

414004 [NCBI]

Taxonomic lineage

Archaea › Thaumarchaeota › Cenarchaeales › Cenarchaeaceae › Cenarchaeum

Protein attributes

Sequence length	894 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 894	894	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000347878
Sites
	Metal binding	587	1	Zinc By similarity
	Metal binding	591	1	Zinc By similarity
	Metal binding	691	1	Zinc By similarity
	Metal binding	695	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RX10 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: A1438318B1D4878F	FASTA	894	97,118
	10 20 30 40 50 60 MEKQEILREF SSDPEKYYTV RLFREEGFER RACSVCGRYF WALDGRPACP EDSDDTYSFI 70 80 90 100 110 120 GDPPAPRAYD YAQAWRTIEE YFVKNGHESV PRYPVVCRWR DDLYFTIASI VDFQRVMGSS 130 140 150 160 170 180 VVFEFPANPL VVPQTCLRFK DLENVGVTGR HFSSFCMIGQ HAVPGNGGYW KDECIDLDYG 190 200 210 220 230 240 LLVRQLGIPK EEVVFVEDVW AGGGSFGPSL EYYVRGLELG NAVFTEFQGE LGNHTTLDRR 250 260 270 280 290 300 IIDMGAGLER FAWITTGTPT AYDCCFGPVM GRLAESLGAD QDSAELAAYY TRVAVNLGRC 310 320 330 340 350 360 GDLNEARRRS AQEAGISDSR MAGAIAPLGE AYMVADHIRT LIFAISDGAL PSNVGGGYNL 370 380 390 400 410 420 RMMLRRVAGA MERTFPGLDL DELVDLHIDY LMGTYPELDG ARQDVKTILD IEASRYGDSK 430 440 450 460 470 480 ERMGKITAKI TDRGRAPGVD ELVTLYESDG ITPEYLIEAG AIQEVPPEFY SRLDELHAPP 490 500 510 520 530 540 PKAAGPGPEL AGLADTEMLF YGEDPPEFEA RVMHSSDGGV VLDRTSFYAR GGGQEPDHGT 550 560 570 580 590 600 IGGFRVTDVS KHGGIILHRL DGGSLAEGST VRCIRDEKRR AGITRNHTST HILNASARGV 610 620 630 640 650 660 LGSWVWQHSA FKEEDHARLD ITHHSPLSAA EVKKIEAAAN GIVKEDRGVS IGYHPRGEAE 670 680 690 700 710 720 QKYGFRIYQG GVVPVSTVRI VTIKGYDDEA CGGTHVKSTG EVGLIRITRT KRIQDGVVRL 730 740 750 760 770 780 EFVSGDAAIE HERTAAARAE GDRAAEEAKG RLQEERDAGR LKSREIIPGM LEEITGGGSA 790 800 810 820 830 840 EGMEVATGPG GRRCLAAGIH DEYFHTSFGK KLVAMDPACA YCAIFEAGPT VRVLAYAGSK 850 860 870 880 890 SGAGADEIVR EVSAVLGGSG GGAAGFAQGG GKDSSKMAEA VARARVLLFG GDNT

References

[1]

"Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum." Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y., Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F. Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006) [PubMed: 17114289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000238 Genomic DNA. Translation: ABK77877.1.
3D structure databases
ProteinModelPortal	A0RX10.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOGENOM	HBG392147.
OMA	MFTNSGM.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 2 hits. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_CENSY

Accession

Primary (citable) accession number: A0RX10

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 9, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program