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Protein

DNA ligase

Gene

lig

Organism
Cenarchaeum symbiosum (strain A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501ATPUniRule annotation
Active sitei252 – 2521N6-AMP-lysine intermediateUniRule annotation
Binding sitei257 – 2571ATPUniRule annotation
Binding sitei272 – 2721ATPUniRule annotation
Binding sitei302 – 3021ATPUniRule annotation
Binding sitei342 – 3421ATPUniRule annotation
Binding sitei417 – 4171ATPUniRule annotation
Binding sitei423 – 4231ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: InterPro
  3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. DNA biosynthetic process Source: InterPro
  4. DNA ligation involved in DNA repair Source: InterPro
  5. DNA recombination Source: UniProtKB-HAMAP
  6. DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.1UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]UniRule annotation
Gene namesi
Name:ligUniRule annotation
Ordered Locus Names:CENSYa_1021
OrganismiCenarchaeum symbiosum (strain A)
Taxonomic identifieri414004 [NCBI]
Taxonomic lineageiArchaeaThaumarchaeotaCenarchaealesCenarchaeaceaeCenarchaeum
ProteomesiUP000000758: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589DNA ligasePRO_0000365244Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliA0RWD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATP-dependent DNA ligase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000036008.
KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RWD6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQFSVLAGSL EKMESTAKRL ELTGILEELL RETPHEVIAQ IVYLIQGKLR
60 70 80 90 100
PEFEGIELGV AEKLAVRAVS KSSGMPAARI EAAYRRDGDL GRAASSILEQ
110 120 130 140 150
KTQTTFLAEE ITVERVYDTL MRIARLEGAR SQDMKMRHIS SLLNDASPRD
160 170 180 190 200
ACYILKLILG TLRLGIAENT VMDALAAAFT GSKSNRPELE RAYNVSSDLG
210 220 230 240 250
RVAEAVSSGG LEAVRGFAVA VFSPIRPMLA DRVRSESEAL EKMGAGLAAE
260 270 280 290 300
YKLDGERVQV HLSGGRVELF SRSLENITAY YPDIVERIPG RLRAREAVLE
310 320 330 340 350
AEAVAVNEET GEFLPFQELM HRRRKYDIDK AVMRYPITVN FFDILYLDGR
360 370 380 390 400
DCLGISYSER RALLEGVVDE DSFARCVPVS TIPDESALED SLENSINAGC
410 420 430 440 450
EGLMLKLPDA PYRAGSRGGY WLKLKREYRN ELGDSLDLVI IGAFFGKGRR
460 470 480 490 500
TGRYGTLLLA TYDDSRDTFP SICKVGTGFT DEDLDQLYQL LSPRVTLKRN
510 520 530 540 550
PRIDSGMEAD VWFDPEVVME VVASEITLSP VHKTALDSVR KGAGLALRFP
560 570 580
KFTGKLRTEK TAEDASTDQE VIALYKSQKK VVPDGQPGV
Length:589
Mass (Da):65,183
Last modified:March 3, 2009 - v2
Checksum:i862D43048CA15D39
GO

Sequence cautioni

The sequence ABK77653.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000238 Genomic DNA. Translation: ABK77653.1. Different initiation.
RefSeqiYP_875957.1. NC_014820.1.

Genome annotation databases

EnsemblBacteriaiABK77653; ABK77653; CENSYa_1021.
GeneIDi6371201.
KEGGicsy:CENSYa_1021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000238 Genomic DNA. Translation: ABK77653.1. Different initiation.
RefSeqiYP_875957.1. NC_014820.1.

3D structure databases

ProteinModelPortaliA0RWD6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK77653; ABK77653; CENSYa_1021.
GeneIDi6371201.
KEGGicsy:CENSYa_1021.

Phylogenomic databases

HOGENOMiHOG000036008.
KOiK10747.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPiMF_00407. DNA_ligase.
InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A.

Entry informationi

Entry nameiDNLI_CENSY
AccessioniPrimary (citable) accession number: A0RWD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 3, 2009
Last sequence update: March 3, 2009
Last modified: February 4, 2015
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.