A0RWD6 (DNLI_CENSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	CENSYa_1021

Organism

Cenarchaeum symbiosum (strain A)

Taxonomic identifier

414004 [NCBI]

Taxonomic lineage

Archaea › Thaumarchaeota › Cenarchaeales › Cenarchaeaceae › Cenarchaeum

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.
Sequence caution	The sequence ABK77653.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	DNA ligase HAMAP MF_00407		PRO_0000365244
Sites
	Active site	252	1	N6-AMP-lysine intermediate By similarity
	Binding site	250	1	ATP By similarity
	Binding site	257	1	ATP By similarity
	Binding site	272	1	ATP By similarity
	Binding site	302	1	ATP By similarity
	Binding site	342	1	ATP By similarity
	Binding site	417	1	ATP By similarity
	Binding site	423	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RWD6 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: 862D43048CA15D39	FASTA	589	65,183
	10 20 30 40 50 60 MQFSVLAGSL EKMESTAKRL ELTGILEELL RETPHEVIAQ IVYLIQGKLR PEFEGIELGV 70 80 90 100 110 120 AEKLAVRAVS KSSGMPAARI EAAYRRDGDL GRAASSILEQ KTQTTFLAEE ITVERVYDTL 130 140 150 160 170 180 MRIARLEGAR SQDMKMRHIS SLLNDASPRD ACYILKLILG TLRLGIAENT VMDALAAAFT 190 200 210 220 230 240 GSKSNRPELE RAYNVSSDLG RVAEAVSSGG LEAVRGFAVA VFSPIRPMLA DRVRSESEAL 250 260 270 280 290 300 EKMGAGLAAE YKLDGERVQV HLSGGRVELF SRSLENITAY YPDIVERIPG RLRAREAVLE 310 320 330 340 350 360 AEAVAVNEET GEFLPFQELM HRRRKYDIDK AVMRYPITVN FFDILYLDGR DCLGISYSER 370 380 390 400 410 420 RALLEGVVDE DSFARCVPVS TIPDESALED SLENSINAGC EGLMLKLPDA PYRAGSRGGY 430 440 450 460 470 480 WLKLKREYRN ELGDSLDLVI IGAFFGKGRR TGRYGTLLLA TYDDSRDTFP SICKVGTGFT 490 500 510 520 530 540 DEDLDQLYQL LSPRVTLKRN PRIDSGMEAD VWFDPEVVME VVASEITLSP VHKTALDSVR 550 560 570 580 KGAGLALRFP KFTGKLRTEK TAEDASTDQE VIALYKSQKK VVPDGQPGV

References

[1]

"Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum." Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y., Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F. Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006) [PubMed: 17114289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000238 Genomic DNA. Translation: ABK77653.1. Different initiation.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOGENOM	HBG570821.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_CENSY

Accession

Primary (citable) accession number: A0RWD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 3, 2009
Last modified:	September 21, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program