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A0RW96

- BIOB_CENSY

UniProt

A0RW96 - BIOB_CENSY

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Protein

Biotin synthase

Gene

bioB

Organism
Cenarchaeum symbiosum (strain A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi105 – 1051Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi138 – 1381Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi267 – 2671Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:CENSYa_0981
OrganismiCenarchaeum symbiosum (strain A)
Taxonomic identifieri414004 [NCBI]
Taxonomic lineageiArchaeaThaumarchaeotaCenarchaealesCenarchaeaceaeCenarchaeum
ProteomesiUP000000758: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 318318Biotin synthasePRO_0000381295Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0RW96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0RW96-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIAECRDKV LGGGELTKDE ARGLMEADVT ELAAAADEIT RRFNGDGVDV
60 70 80 90 100
EQLNNIKRDG CSEDCTFCGQ SAFYDADKEP HPLPEPEEVV RAALKAKKEE
110 120 130 140 150
ASSYCLVAAW REPTPEGFEK VCTIIQEINT HVGISVECSL GFLTRERAAR
160 170 180 190 200
LKGLGVKRYN HNLETARSKF PEICSTHTYE DRLDTLEIAR EAGLELCTGG
210 220 230 240 250
IIGMGESRGQ RIELAMELAR IRPEEATVNI LVPVQGTPME LQAPLPPGEA
260 270 280 290 300
ERFFALVRFL LPRSVVKISG GREKALDDDG RAILRGGANG IITSGYLTMG
310
GNDSSADMEM IREAGLEA
Length:318
Mass (Da):34,615
Last modified:January 9, 2007 - v1
Checksum:iF379432CF10A4A66
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000238 Genomic DNA. Translation: ABK77613.1.
RefSeqiYP_875917.1. NC_014820.1.

Genome annotation databases

EnsemblBacteriaiABK77613; ABK77613; CENSYa_0981.
GeneIDi6371161.
KEGGicsy:CENSYa_0981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000238 Genomic DNA. Translation: ABK77613.1 .
RefSeqi YP_875917.1. NC_014820.1.

3D structure databases

ProteinModelPortali A0RW96.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK77613 ; ABK77613 ; CENSYa_0981 .
GeneIDi 6371161.
KEGGi csy:CENSYa_0981.

Phylogenomic databases

HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: A.

Entry informationi

Entry nameiBIOB_CENSY
AccessioniPrimary (citable) accession number: A0RW96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: October 1, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3