A0RUA5 (AMPA_CENSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	CENSYa_0283

Organism

Cenarchaeum symbiosum (strain A)

Taxonomic identifier

414004 [NCBI]

Taxonomic lineage

Archaea › Thaumarchaeota › Cenarchaeales › Cenarchaeaceae › Cenarchaeum

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098312
Sites
	Active site	263	1	Potential
	Active site	337	1	Potential
	Metal binding	251	1	Manganese 2 By similarity
	Metal binding	256	1	Manganese 1 By similarity
	Metal binding	256	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	333	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 1 By similarity
	Metal binding	335	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RUA5 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: F0E23B607ED79373	FASTA	488	50,571
	10 20 30 40 50 60 MKITADASDP AKRKTAALCG FVLEGAAGAA GLGGPGLDDL IKEAIGKNGG KKGRISMTGT 70 80 90 100 110 120 PGMPSERVIL AGLGPAGKVT SDGIRSEAGR LARQVRDMGL ADFAVAAPSI LDPAEEAALI 130 140 150 160 170 180 VEGAELALYS FDEFKAEKAG NSPRLHILGG GPSAARAIKD AQSITAGVAF ARGLANMPPN 190 200 210 220 230 240 ECPPDKLGRA AVKMCTGKKM RCIVLKKPEL KKGGFGGIIA VGQGSSNEPR LIIVEYNGGK 250 260 270 280 290 300 KGQKPIVLVG KAVTFDTGGI SIKPGEKMDE MKFDKCGGCT VLGIMKAVED LALPVNVIGI 310 320 330 340 350 360 VPSVENMPGG SSYRPGDIVK LYSGKTAEIL NTDAEGRLIL ADALSYGEKK YAPSEIIDFA 370 380 390 400 410 420 TLTGACIVAL GNNVAGMVSN DDAFAKRIEG ASERTAEEVW RLPINDDYMS MIKSEVADMR 430 440 450 460 470 480 NLGMGRAAGT ITAAAFLKNA IGDTPWVHLD IAGTAWNQPA TKKKPYNPGG ATGFGVRLVA GYLKGRAQ

References

[1]

"Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum." Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y., Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F. Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006) [PubMed: 17114289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000238 Genomic DNA. Translation: ABK76922.1.
3D structure databases
ProteinModelPortal	A0RUA5.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	IMPCTEN.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_CENSY

Accession

Primary (citable) accession number: A0RUA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program