Reviewed, UniProtKB/Swiss-Prot A0RTV6 (RIFK_CENSY)

Names and origin

Protein names

Recommended name:
    Riboflavin kinase
      Short name=RFK
    EC=2.7.1.161
Alternative name(s):
    CTP:riboflavin 5'-phosphotransferase
    CTP-dependent riboflavin kinase
    Flavokinase

Gene names

Name:	rfk
Ordered Locus Names:	CENSYa_0128

Organism

Cenarchaeum symbiosum [Complete proteome] [HAMAP]

Taxonomic identifier

46770 [NCBI]

Taxonomic lineage

Archaea › Thaumarchaeota › Cenarchaeales › Cenarchaeaceae › Cenarchaeum

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity.
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	FMN biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: HAMAP riboflavin kinase activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Riboflavin kinase HAMAP MF_01285		PRO_0000322083
Regions
	Nucleotide binding	69 – 74	6	CDP By similarity
	Nucleotide binding	169 – 172	4	CDP By similarity
	Region	1 – 59	59	H-T-H motif-like HAMAP MF_01285
	Region	60 – 193	134	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	98	1	Magnesium By similarity
	Metal binding	100	1	Magnesium By similarity
	Binding site	156	1	FMN By similarity
	Binding site	164	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RTV6-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: C390EBC862001E2A	FASTA	193	21,446
	10 20 30 40 50 60 MGISQQAASQ HLRELEDEGL ITRNAEGKGI SVMVTDKGRH ELLRVYNILH DSLHSRPDHV 70 80 90 100 110 120 EITGTLVSGM NEGAYYMSRE GYTGQFQERL GYVPFPGTLN VDTDRKHGPE IARLDGMNGT 130 140 150 160 170 180 IIDGFTDGKR SYGWVKCFAG TLNGTIPCHL IRLERTHHGS STVELISKLD IRKETGLDDG 190 GKITIRIPLE QED

References

[1]

"Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum symbiosum." Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y., Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F. Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006) [PubMed: 17114289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DP000238 Genomic DNA. Translation: ABK76773.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus CENSYa_0128 in contig DP000238_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GAYYVRQ.
Family and domain databases
HAMAP	MF_01285. Fused. [Tree]
InterPro	IPR001845. HTH_ArsR. IPR002834. Riboflavin_kinase_arc. [Graphical view]
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProDom	PD015839. DUF120. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS50987. HTH_ARSR_2. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_CENSY

Accession

Primary (citable) accession number: A0RTV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)