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Protein

2-isopropylmalate synthase

Gene

leuA

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).UniRule annotation

Catalytic activityi

Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA.UniRule annotation

Pathwayi

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

BioCyciCFET360106:GHTH-1342-MONOMER.
UniPathwayiUPA00048; UER00070.

Names & Taxonomyi

Protein namesi
Recommended name:
2-isopropylmalate synthaseUniRule annotation (EC:2.3.3.13UniRule annotation)
Alternative name(s):
Alpha-IPM synthaseUniRule annotation
Alpha-isopropylmalate synthaseUniRule annotation
Gene namesi
Name:leuAUniRule annotation
Ordered Locus Names:CFF8240_1344
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000760 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5065062-isopropylmalate synthasePRO_1000149159Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi360106.CFF8240_1344.

Structurei

3D structure databases

ProteinModelPortaliA0RQK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RQK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNKIIVFD TTLRDGEQSP GASMNTEEKI QIALQLERLG VDVMEAGFAA
60 70 80 90 100
ASPGDFDAIN QIAKQIHSIR IASLARALEK DIKAAGEAIS PAKNRRIHTF
110 120 130 140 150
IATSPIHMEH KLKMTPDEVI KRAVEAVKYA KTFVDDVEFS CEDAGRSDIV
160 170 180 190 200
FLKEICAAVV EAGARTLNLP DTVGFRMPDE IYNMVKSMVD FIGDRAIISV
210 220 230 240 250
HNHNDLGLAV ANTLASIKAG ARQVECTING LGERAGNAAL EEIVMTIRTR
260 270 280 290 300
SDEFAPLYTD IVTKEIYATS RLVASITGIE PQPNKAIVGK NAFAHESGIH
310 320 330 340 350
QDGMLKCAQT YEIIKAEDIG AEKNSLVLGK HSGRHAFKDK LINLGFDLDD
360 370 380 390 400
NEINEAFIKF KELCDKKKEI FDDDIRALVS HEIIKIPEIY SIQTLSTSSC
410 420 430 440 450
NAGHSSAAVS IKFSDNIISD AALGNGTADA IFKVIDRISG ISGELKDYKV
460 470 480 490 500
NAVSQGKDAL AKITVKVVFE GSSCATIGHG LDIDTMMASA KAYVSALNSY

LSMKNR
Length:506
Mass (Da):54,896
Last modified:January 9, 2007 - v1
Checksum:iEA0FBEE7E49F01E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82096.1.
RefSeqiWP_002850159.1. NC_008599.1.
YP_892490.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK82096; ABK82096; CFF8240_1344.
GeneIDi4539073.
KEGGicff:CFF8240_1344.
PATRICi20037206. VBICamFet25865_1308.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82096.1.
RefSeqiWP_002850159.1. NC_008599.1.
YP_892490.1. NC_008599.1.

3D structure databases

ProteinModelPortaliA0RQK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360106.CFF8240_1344.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK82096; ABK82096; CFF8240_1344.
GeneIDi4539073.
KEGGicff:CFF8240_1344.
PATRICi20037206. VBICamFet25865_1308.

Phylogenomic databases

eggNOGiCOG0119.
HOGENOMiHOG000046859.
KOiK01649.
OMAiWSVHCHN.
OrthoDBiEOG6CGCF3.

Enzyme and pathway databases

UniPathwayiUPA00048; UER00070.
BioCyciCFET360106:GHTH-1342-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01025. LeuA_type1.
InterProiIPR013709. 2-isopropylmalate_synth_dimer.
IPR002034. AIPM/Hcit_synth_CS.
IPR013785. Aldolase_TIM.
IPR005671. LeuA_bact_synth.
IPR000891. PYR_CT.
[Graphical view]
PfamiPF00682. HMGL-like. 1 hit.
PF08502. LeuA_dimer. 1 hit.
[Graphical view]
SMARTiSM00917. LeuA_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF110921. SSF110921. 1 hit.
TIGRFAMsiTIGR00973. leuA_bact. 1 hit.
PROSITEiPS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit.
PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit.
PS50991. PYR_CT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
    Fouts D.E., Nelson K.E.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 82-40.

Entry informationi

Entry nameiLEU1_CAMFF
AccessioniPrimary (citable) accession number: A0RQK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: May 27, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.