Reviewed, UniProtKB/Swiss-Prot A0RQC4 (ISPH_CAMFF)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	CFF8240_1251

Organism

Campylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]

Taxonomic identifier

360106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021095

Sequences

	Sequence		Length	Mass (Da)
	A0RQC4-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: C8A94A70BB199F19	FASTA	279	31,466
	10 20 30 40 50 60 MKIELAKSYG FCFGVKRAIK IAENSKNAST IGELIHNSLE IDRLKNNFNV KTLKDISELK 70 80 90 100 110 120 NEKKAIIRTH GITKEGLANL KSRDVEIIDA TCPFVTKPQQ IVEKMSSEGY EIVFFGDINH 130 140 150 160 170 180 PEVKGVMSYS SKNVYVILDE SELETVKLPS KIAVVSQTTK KIEKFTKIVS YLMQRVKEVR 190 200 210 220 230 240 VFNTICNATL ENQEAVRELS SRADVMVIIG GKNSSNTKQL YLISKNLCPD SYLIESENEL 250 260 270 ELKWFQNKKL CGISAGASTP EWVIQNVINK LENLTHKDS

References

[1]	"Sequence of Campylobacter fetus subsp. fetus 82-40." Fouts D.E., Nelson K.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000487 Genomic DNA. Translation: ABK82316.1.
RefSeq	YP_892407.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0RQC4.
Genome annotation databases
GeneID	4539553.
GenomeReviews	Gene locus CFF8240_1251 in contig CP000487_GR.
KEGG	cff:CFF8240_1251.
NMPDR	fig|360106.5.peg.1203.
TIGR	CFF8240_1251.
Phylogenomic databases
OMA	KNDICYA.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_CAMFF

Accession

Primary (citable) accession number: A0RQC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)