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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei126 – 1261S-adenosyl-L-methionineUniRule annotation
Binding sitei151 – 1511S-adenosyl-L-methionineUniRule annotation
Binding sitei178 – 1781S-adenosyl-L-methionineUniRule annotation
Binding sitei204 – 2041SubstrateUniRule annotation
Binding sitei234 – 2341SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCFET360106:GHTH-1216-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:CFF8240_1218
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000760 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395tRNA (guanine-N(7)-)-methyltransferasePRO_0000288132Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi360106.CFF8240_1218.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000252163.
KOiK03439.
OMAiSKYEARW.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RQ93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPNFVATSLK NLKFPFGADG VEFLWSAKAG DESLIYTKTG KKEFFLLVKD
60 70 80 90 100
KKTEFVVKSD KLTRPASLGT IQKALSVYKD LNVLDLKSST IAIKNEKQLA
110 120 130 140 150
KKEFILNDIQ FLEKLKSGEF KKIFVEIGFG SGRHLLYQAK TDENTLVVGI
160 170 180 190 200
EVYKRSCEQV NNLALSMGLK NVILLNLDAR LVMSLLHSNS VDRLFLHFPV
210 220 230 240 250
PWEKSEKRRV VSAEFANECQ RVLKSGGSFE LRSDDKNYTD FTISCFLNLK
260 270 280 290 300
EAKMEIYKNR FLDVSSKYED RWIKQNRDIY DVVFTNLIVS EQKVLHGDFE
310 320 330 340 350
FGTVLEEDIL SKFENKTIKK DDYFIHMERI YKKDSSEKNG GLLLRVAFGS
360 370 380 390
FYRPEHCFIL VENSKASYFI KKPLLTYENL KAHSTLKEYL SCSTS
Length:395
Mass (Da):45,516
Last modified:January 9, 2007 - v1
Checksum:iB46D6A5ACF835BD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82983.1.
RefSeqiYP_892376.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK82983; ABK82983; CFF8240_1218.
KEGGicff:CFF8240_1218.
PATRICi20036954. VBICamFet25865_1191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82983.1.
RefSeqiYP_892376.1. NC_008599.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360106.CFF8240_1218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK82983; ABK82983; CFF8240_1218.
KEGGicff:CFF8240_1218.
PATRICi20036954. VBICamFet25865_1191.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000252163.
KOiK03439.
OMAiSKYEARW.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciCFET360106:GHTH-1216-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
    Fouts D.E., Nelson K.E.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 82-40.

Entry informationi

Entry nameiTRMB_CAMFF
AccessioniPrimary (citable) accession number: A0RQ93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 9, 2007
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.