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A0RQ93 (TRMB_CAMFF) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:CFF8240_1218
OrganismCampylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]
Taxonomic identifier360106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288132

Sites

Binding site1261S-adenosyl-L-methionine By similarity
Binding site1511S-adenosyl-L-methionine By similarity
Binding site1781S-adenosyl-L-methionine By similarity
Binding site2041Substrate By similarity
Binding site2341Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A0RQ93 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: B46D6A5ACF835BD2

FASTA39545,516
        10         20         30         40         50         60 
MPNFVATSLK NLKFPFGADG VEFLWSAKAG DESLIYTKTG KKEFFLLVKD KKTEFVVKSD 

        70         80         90        100        110        120 
KLTRPASLGT IQKALSVYKD LNVLDLKSST IAIKNEKQLA KKEFILNDIQ FLEKLKSGEF 

       130        140        150        160        170        180 
KKIFVEIGFG SGRHLLYQAK TDENTLVVGI EVYKRSCEQV NNLALSMGLK NVILLNLDAR 

       190        200        210        220        230        240 
LVMSLLHSNS VDRLFLHFPV PWEKSEKRRV VSAEFANECQ RVLKSGGSFE LRSDDKNYTD 

       250        260        270        280        290        300 
FTISCFLNLK EAKMEIYKNR FLDVSSKYED RWIKQNRDIY DVVFTNLIVS EQKVLHGDFE 

       310        320        330        340        350        360 
FGTVLEEDIL SKFENKTIKK DDYFIHMERI YKKDSSEKNG GLLLRVAFGS FYRPEHCFIL 

       370        380        390 
VENSKASYFI KKPLLTYENL KAHSTLKEYL SCSTS 

« Hide

References

[1]"Sequence of Campylobacter fetus subsp. fetus 82-40."
Fouts D.E., Nelson K.E.
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 82-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000487 Genomic DNA. Translation: ABK82983.1.
RefSeqYP_892376.1. NC_008599.1.

3D structure databases

ProteinModelPortalA0RQ93.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360106.CFF8240_1218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK82983; ABK82983; CFF8240_1218.
GeneID4538618.
KEGGcff:CFF8240_1218.
PATRIC20036954. VBICamFet25865_1191.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000252163.
KOK03439.
OMASKYEARW.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycCFET360106:GHTH-1216-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CAMFF
AccessionPrimary (citable) accession number: A0RQ93
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 9, 2007
Last modified: June 11, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways