Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Gene

mnmC

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.UniRule annotation

Cofactori

FADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCUniRule annotation
Short name:
tRNA mnm(5)s(2)U biosynthesis bifunctional proteinUniRule annotation
Including the following 2 domains:
tRNA (mnm(5)s(2)U34)-methyltransferaseUniRule annotation (EC:2.1.1.61UniRule annotation)
FAD-dependent cmnm(5)s(2)U34 oxidoreductaseUniRule annotation (EC:1.5.-.-UniRule annotation)
Gene namesi
Name:mnmCUniRule annotation
Ordered Locus Names:CFF8240_1208
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000760 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003479681 – 612tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCAdd BLAST612

Structurei

3D structure databases

ProteinModelPortaliA0RQ83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 218tRNA (mnm(5)s(2)U34)-methyltransferaseAdd BLAST218
Regioni244 – 612FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd BLAST369

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the DAO family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252178.
KOiK15461.
OMAiVGFRGYS.
OrthoDBiPOG091H04VA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.

Sequencei

Sequence statusi: Complete.

A0RQ83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITFRGDGLY NSKFDDIYFN TNEPLIECEH TYSSVLDEIN AKFIVVAEAG
60 70 80 90 100
FGTGLNFFST VLKFLSLNST ELHYIAVEKY PFKKSELREI YLKFEILKPF
110 120 130 140 150
FDEFIEQYEI LDGALIRIKL LNERVILDLY FGDILDAFDE LSFRADAWYL
160 170 180 190 200
DGFSPTKNPD MWSKEVFDRL SKFCKNRAKV RTFSSAKIVQ NRFLEHGFSI
210 220 230 240 250
KKLKGHYKKR EILEASLEHS SPKILKEPWY ALPNISKFSD VLIIGAGVAG
260 270 280 290 300
LAAAFKFKKA GFNVCIAEKM SEAATNGSSN LAGILMPLIT KPKVALGNMH
310 320 330 340 350
MSAFLFARHF YANSPFADFC GVYDYGVNDL EKTRLSLWDS EIFKFENDFE
360 370 380 390 400
PYPRAFIKSA AQIRPKELCM SLASEFDIKY GYEFESIEKS TGGYVVKFKN
410 420 430 440 450
SKNIFSSLVI FAMGDVSTNL FQNVFADEFM QLSSVRGQVT HLNKVLNLNS
460 470 480 490 500
AFSARGYMCK DVRGIQVVGA TYDRNDNRSA ARATDDEKNI DSLSEFITNL
510 520 530 540 550
DVKVVGSNVG FRGYSGDRFP IVGAVHNASE FKKIYKSLLW TKHSKNNEFA
560 570 580 590 600
VHHENILISS AHGSRGLGTA IFGAEILLDI ALNRPVCTTN SILNSLNPAR
610
FLVRKLKRGL VR
Length:612
Mass (Da):69,218
Last modified:January 9, 2007 - v1
Checksum:i39253B9EFA5D8F3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82863.1.
RefSeqiWP_011732120.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK82863; ABK82863; CFF8240_1208.
GeneIDi4539163.
KEGGicff:CFF8240_1208.
PATRICi20036934. VBICamFet25865_1181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82863.1.
RefSeqiWP_011732120.1. NC_008599.1.

3D structure databases

ProteinModelPortaliA0RQ83.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK82863; ABK82863; CFF8240_1208.
GeneIDi4539163.
KEGGicff:CFF8240_1208.
PATRICi20036934. VBICamFet25865_1181.

Phylogenomic databases

HOGENOMiHOG000252178.
KOiK15461.
OMAiVGFRGYS.
OrthoDBiPOG091H04VA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMC_CAMFF
AccessioniPrimary (citable) accession number: A0RQ83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.