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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCFET360106:GHTH-1159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CFF8240_1161
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000760 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signalUniRule annotationAdd
BLAST
Chaini31 – 925895Periplasmic nitrate reductasePRO_1000069712Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0RQ36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiETLWRFN.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0RQ36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRREFIKSS AAAAACSAAG IAVPSSLSAA ENQDGWRWDK SACRFCGTGC
60 70 80 90 100
GIMVATKNGK IVAVKGDPLA PVNRGLNCIK GYFNAKIMYG EDRITKPLLR
110 120 130 140 150
VNANGEFDKK GKFQEVSWKR AFDEMEKQFR KTYAELGPTG IGVFGSGQYT
160 170 180 190 200
IQEGYAISKL IKGGFRSHNI DPNARHCMAS AVVGFMQTFG IDEPAGCYDD
210 220 230 240 250
IELTDTIVTW GANMAEMHPI LWSRVSDRKL QNSDKVKVVN LSTYSTRTSN
260 270 280 290 300
IADIEIIFTP HTDLAIWNYI AREIVYNHPE AIDEEFVKAN CVFTTGPVDI
310 320 330 340 350
GYGMRANIKH PKYLPSELDT AAKEKSTVLS ENEGVTLAYL GMKAGDTLEN
360 370 380 390 400
KSTGAPDKHW IIQYEDFKKA LAPYTLDFVA KLAKGDPNED LEEFKKKLKA
410 420 430 440 450
LADLYIEKNR KVVSFWTMGM NQHTRGVWVN EQSYMVHFLL GKQAKPGSGA
460 470 480 490 500
FSLTGQPSAC GTAREVGTFS HRLPADMVVA NPKHREITEK IWKIPAGTIN
510 520 530 540 550
PKPGAPYMKI MRDLEDGKVK FVWVHVNNPW QNSANANHWI KAAREMDNFI
560 570 580 590 600
VVSDPYPGIS AKVGDLILPT AMIYEKWGAY GNAERRTQHW RQQVLPVGDA
610 620 630 640 650
MSDTWQYMEF AKRFKLKDFW GEVKVDGKLT LPNVLDKAVA MGYNPENTLF
660 670 680 690 700
EVLFANKSAM KFSSDDKIMA GYDNTEVFGD SRNVVGSDGN VFKGYGFFVQ
710 720 730 740 750
KYLWEEYREF GLGHGHDLAD FDTYHKVRGL RWPVVDGKET LWRFNTKYDV
760 770 780 790 800
YAKKDNPNGD FSFYGNKNGA LVTGDLAKAT SKEKEALKSR AKIFFRPYMD
810 820 830 840 850
PPEMPSKDYP LWLCTGRVLE HWHSGTMTMR VPELYRAVPE ALCFMNEIDG
860 870 880 890 900
NKFGIKQNDI IWVESRRGKV KARVDFRGRN KPAEGLIYVP WFDENVFINK
910 920
VCLDATDPLS KQTDFKKCAV KIYKA
Length:925
Mass (Da):104,255
Last modified:January 9, 2007 - v1
Checksum:i6421C720F2CE60BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82549.1.
RefSeqiWP_011732100.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK82549; ABK82549; CFF8240_1161.
GeneIDi4539058.
KEGGicff:CFF8240_1161.
PATRICi20036840. VBICamFet25865_1134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82549.1.
RefSeqiWP_011732100.1. NC_008599.1.

3D structure databases

ProteinModelPortaliA0RQ36.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK82549; ABK82549; CFF8240_1161.
GeneIDi4539058.
KEGGicff:CFF8240_1161.
PATRICi20036840. VBICamFet25865_1134.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiETLWRFN.
OrthoDBiEOG6CVV7G.

Enzyme and pathway databases

BioCyciCFET360106:GHTH-1159-MONOMER.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
    Fouts D.E., Nelson K.E.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 82-40.

Entry informationi

Entry nameiNAPA_CAMFF
AccessioniPrimary (citable) accession number: A0RQ36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: July 22, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.