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A0RQ36 (NAPA_CAMFF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:CFF8240_1161
OrganismCampylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]
Taxonomic identifier360106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length925 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030Tat-type signal Potential
Chain31 – 925895Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000069712

Regions

Domain36 – 92574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding431Iron-sulfur (4Fe-4S) By similarity
Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding781Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A0RQ36 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 6421C720F2CE60BB

FASTA925104,255
        10         20         30         40         50         60 
MDRREFIKSS AAAAACSAAG IAVPSSLSAA ENQDGWRWDK SACRFCGTGC GIMVATKNGK 

        70         80         90        100        110        120 
IVAVKGDPLA PVNRGLNCIK GYFNAKIMYG EDRITKPLLR VNANGEFDKK GKFQEVSWKR 

       130        140        150        160        170        180 
AFDEMEKQFR KTYAELGPTG IGVFGSGQYT IQEGYAISKL IKGGFRSHNI DPNARHCMAS 

       190        200        210        220        230        240 
AVVGFMQTFG IDEPAGCYDD IELTDTIVTW GANMAEMHPI LWSRVSDRKL QNSDKVKVVN 

       250        260        270        280        290        300 
LSTYSTRTSN IADIEIIFTP HTDLAIWNYI AREIVYNHPE AIDEEFVKAN CVFTTGPVDI 

       310        320        330        340        350        360 
GYGMRANIKH PKYLPSELDT AAKEKSTVLS ENEGVTLAYL GMKAGDTLEN KSTGAPDKHW 

       370        380        390        400        410        420 
IIQYEDFKKA LAPYTLDFVA KLAKGDPNED LEEFKKKLKA LADLYIEKNR KVVSFWTMGM 

       430        440        450        460        470        480 
NQHTRGVWVN EQSYMVHFLL GKQAKPGSGA FSLTGQPSAC GTAREVGTFS HRLPADMVVA 

       490        500        510        520        530        540 
NPKHREITEK IWKIPAGTIN PKPGAPYMKI MRDLEDGKVK FVWVHVNNPW QNSANANHWI 

       550        560        570        580        590        600 
KAAREMDNFI VVSDPYPGIS AKVGDLILPT AMIYEKWGAY GNAERRTQHW RQQVLPVGDA 

       610        620        630        640        650        660 
MSDTWQYMEF AKRFKLKDFW GEVKVDGKLT LPNVLDKAVA MGYNPENTLF EVLFANKSAM 

       670        680        690        700        710        720 
KFSSDDKIMA GYDNTEVFGD SRNVVGSDGN VFKGYGFFVQ KYLWEEYREF GLGHGHDLAD 

       730        740        750        760        770        780 
FDTYHKVRGL RWPVVDGKET LWRFNTKYDV YAKKDNPNGD FSFYGNKNGA LVTGDLAKAT 

       790        800        810        820        830        840 
SKEKEALKSR AKIFFRPYMD PPEMPSKDYP LWLCTGRVLE HWHSGTMTMR VPELYRAVPE 

       850        860        870        880        890        900 
ALCFMNEIDG NKFGIKQNDI IWVESRRGKV KARVDFRGRN KPAEGLIYVP WFDENVFINK 

       910        920 
VCLDATDPLS KQTDFKKCAV KIYKA 

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References

[1]"Sequence of Campylobacter fetus subsp. fetus 82-40."
Fouts D.E., Nelson K.E.
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 82-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000487 Genomic DNA. Translation: ABK82549.1.
RefSeqYP_892319.1. NC_008599.1.

3D structure databases

ProteinModelPortalA0RQ36.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360106.CFF8240_1161.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK82549; ABK82549; CFF8240_1161.
GeneID4539058.
KEGGcff:CFF8240_1161.
PATRIC20036840. VBICamFet25865_1134.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAFNQHQRG.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycCFET360106:GHTH-1159-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CAMFF
AccessionPrimary (citable) accession number: A0RQ36
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families