Reviewed, UniProtKB/Swiss-Prot A0RQ36 (NAPA_CAMFF)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	CFF8240_1161

Organism

Campylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]

Taxonomic identifier

360106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	925 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Tat-type signal Potential
	Chain	31 – 925	895	Periplasmic nitrate reductase HAMAP MF_01630		PRO_1000069712
Sites
	Metal binding	43	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RQ36-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 6421C720F2CE60BB	FASTA	925	104,255
	10 20 30 40 50 60 MDRREFIKSS AAAAACSAAG IAVPSSLSAA ENQDGWRWDK SACRFCGTGC GIMVATKNGK 70 80 90 100 110 120 IVAVKGDPLA PVNRGLNCIK GYFNAKIMYG EDRITKPLLR VNANGEFDKK GKFQEVSWKR 130 140 150 160 170 180 AFDEMEKQFR KTYAELGPTG IGVFGSGQYT IQEGYAISKL IKGGFRSHNI DPNARHCMAS 190 200 210 220 230 240 AVVGFMQTFG IDEPAGCYDD IELTDTIVTW GANMAEMHPI LWSRVSDRKL QNSDKVKVVN 250 260 270 280 290 300 LSTYSTRTSN IADIEIIFTP HTDLAIWNYI AREIVYNHPE AIDEEFVKAN CVFTTGPVDI 310 320 330 340 350 360 GYGMRANIKH PKYLPSELDT AAKEKSTVLS ENEGVTLAYL GMKAGDTLEN KSTGAPDKHW 370 380 390 400 410 420 IIQYEDFKKA LAPYTLDFVA KLAKGDPNED LEEFKKKLKA LADLYIEKNR KVVSFWTMGM 430 440 450 460 470 480 NQHTRGVWVN EQSYMVHFLL GKQAKPGSGA FSLTGQPSAC GTAREVGTFS HRLPADMVVA 490 500 510 520 530 540 NPKHREITEK IWKIPAGTIN PKPGAPYMKI MRDLEDGKVK FVWVHVNNPW QNSANANHWI 550 560 570 580 590 600 KAAREMDNFI VVSDPYPGIS AKVGDLILPT AMIYEKWGAY GNAERRTQHW RQQVLPVGDA 610 620 630 640 650 660 MSDTWQYMEF AKRFKLKDFW GEVKVDGKLT LPNVLDKAVA MGYNPENTLF EVLFANKSAM 670 680 690 700 710 720 KFSSDDKIMA GYDNTEVFGD SRNVVGSDGN VFKGYGFFVQ KYLWEEYREF GLGHGHDLAD 730 740 750 760 770 780 FDTYHKVRGL RWPVVDGKET LWRFNTKYDV YAKKDNPNGD FSFYGNKNGA LVTGDLAKAT 790 800 810 820 830 840 SKEKEALKSR AKIFFRPYMD PPEMPSKDYP LWLCTGRVLE HWHSGTMTMR VPELYRAVPE 850 860 870 880 890 900 ALCFMNEIDG NKFGIKQNDI IWVESRRGKV KARVDFRGRN KPAEGLIYVP WFDENVFINK 910 920 VCLDATDPLS KQTDFKKCAV KIYKA

References

[1]	"Sequence of Campylobacter fetus subsp. fetus 82-40." Fouts D.E., Nelson K.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000487 Genomic DNA. Translation: ABK82549.1.
RefSeq	YP_892319.1.
3D structure databases
SMR	A0RQ36. Positions 36-924.
ModBase	Search...
Protein-protein interaction databases
STRING	A0RQ36.
Genome annotation databases
GeneID	4539058.
GenomeReviews	Gene locus CFF8240_1161 in contig CP000487_GR.
KEGG	cff:CFF8240_1161.
NMPDR	fig|360106.5.peg.1114.
TIGR	CFF8240_1161.
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HBG715033.
OMA	IISAPYE.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_CAMFF

Accession

Primary (citable) accession number: A0RQ36

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 9, 2007
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)