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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi78Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei80Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei148Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei173Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei418Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei422Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei528Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei576Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei603Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei891Substrate; via amide nitrogenUniRule annotation1
Binding sitei899Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei916Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:CFF8240_1161
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_100006971231 – 925Periplasmic nitrate reductaseUniRule annotationAdd BLAST895

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0RQ36
SMRiA0RQ36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 924Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 217Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni553 – 554Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni815 – 824Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000031441
KOiK02567
OMAiTQHWRQQ
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 2 hits
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A0RQ36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRREFIKSS AAAAACSAAG IAVPSSLSAA ENQDGWRWDK SACRFCGTGC
60 70 80 90 100
GIMVATKNGK IVAVKGDPLA PVNRGLNCIK GYFNAKIMYG EDRITKPLLR
110 120 130 140 150
VNANGEFDKK GKFQEVSWKR AFDEMEKQFR KTYAELGPTG IGVFGSGQYT
160 170 180 190 200
IQEGYAISKL IKGGFRSHNI DPNARHCMAS AVVGFMQTFG IDEPAGCYDD
210 220 230 240 250
IELTDTIVTW GANMAEMHPI LWSRVSDRKL QNSDKVKVVN LSTYSTRTSN
260 270 280 290 300
IADIEIIFTP HTDLAIWNYI AREIVYNHPE AIDEEFVKAN CVFTTGPVDI
310 320 330 340 350
GYGMRANIKH PKYLPSELDT AAKEKSTVLS ENEGVTLAYL GMKAGDTLEN
360 370 380 390 400
KSTGAPDKHW IIQYEDFKKA LAPYTLDFVA KLAKGDPNED LEEFKKKLKA
410 420 430 440 450
LADLYIEKNR KVVSFWTMGM NQHTRGVWVN EQSYMVHFLL GKQAKPGSGA
460 470 480 490 500
FSLTGQPSAC GTAREVGTFS HRLPADMVVA NPKHREITEK IWKIPAGTIN
510 520 530 540 550
PKPGAPYMKI MRDLEDGKVK FVWVHVNNPW QNSANANHWI KAAREMDNFI
560 570 580 590 600
VVSDPYPGIS AKVGDLILPT AMIYEKWGAY GNAERRTQHW RQQVLPVGDA
610 620 630 640 650
MSDTWQYMEF AKRFKLKDFW GEVKVDGKLT LPNVLDKAVA MGYNPENTLF
660 670 680 690 700
EVLFANKSAM KFSSDDKIMA GYDNTEVFGD SRNVVGSDGN VFKGYGFFVQ
710 720 730 740 750
KYLWEEYREF GLGHGHDLAD FDTYHKVRGL RWPVVDGKET LWRFNTKYDV
760 770 780 790 800
YAKKDNPNGD FSFYGNKNGA LVTGDLAKAT SKEKEALKSR AKIFFRPYMD
810 820 830 840 850
PPEMPSKDYP LWLCTGRVLE HWHSGTMTMR VPELYRAVPE ALCFMNEIDG
860 870 880 890 900
NKFGIKQNDI IWVESRRGKV KARVDFRGRN KPAEGLIYVP WFDENVFINK
910 920
VCLDATDPLS KQTDFKKCAV KIYKA
Length:925
Mass (Da):104,255
Last modified:January 9, 2007 - v1
Checksum:i6421C720F2CE60BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA Translation: ABK82549.1
RefSeqiWP_011732100.1, NC_008599.1

Genome annotation databases

EnsemblBacteriaiABK82549; ABK82549; CFF8240_1161
KEGGicff:CFF8240_1161

Entry informationi

Entry nameiNAPA_CAMFF
AccessioniPrimary (citable) accession number: A0RQ36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 9, 2007
Last modified: March 28, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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