ID   TGT_CAMFF               Reviewed;         374 AA.
AC   A0RPL5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   16-JUN-2009, entry version 16.
DE   RecName: Full=Queuine tRNA-ribosyltransferase;
DE            EC=2.4.2.29;
DE   AltName: Full=tRNA-guanine transglycosylase;
DE   AltName: Full=Guanine insertion enzyme;
GN   Name=tgt; OrderedLocusNames=CFF8240_0980;
OS   Campylobacter fetus subsp. fetus (strain 82-40).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=360106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Fouts D.E., Nelson K.E.;
RT   "Sequence of Campylobacter fetus subsp. fetus 82-40.";
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Exchanges the guanine residue with 7-aminomethyl-7-
CC       deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His
CC       and -Tyr). After this exchange, a cyclopentendiol moiety is
CC       attached to the 7-aminomethyl group of 7-deazaguanine, resulting
CC       in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-
CC       dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: [tRNA]-guanine + queuine = [tRNA]-queuine +
CC       guanine.
CC   -!- CATALYTIC ACTIVITY: [tRNA]-guanine + 7-aminomethyl-7-carbaguanine
CC       = [tRNA]-7-aminomethyl-7-carbaguanine + guanine.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: tRNA modification; queuosine-tRNA biosynthesis.
CC   -!- SIMILARITY: Belongs to the queuine tRNA-ribosyltransferase family.
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DR   EMBL; CP000487; ABK81825.1; -; Genomic_DNA.
DR   RefSeq; YP_892148.1; -.
DR   GeneID; 4537752; -.
DR   GenomeReviews; CP000487_GR; CFF8240_0980.
DR   KEGG; cff:CFF8240_0980; -.
DR   NMPDR; fig|360106.5.peg.934; -.
DR   TIGR; CFF8240_0980; -.
DR   OMA; A0RPL5; ECVRLPA.
DR   GO; GO:0008479; F:queuine tRNA-ribosyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00168; -; 1.
DR   InterPro; IPR004803; QtRNA_ribo_trans.
DR   InterPro; IPR002616; tRNA_ribo_trans.
DR   Gene3D; G3DSA:3.20.20.105; tRNA_ribo_trans; 1.
DR   PANTHER; PTHR11962; tRNA_ribo_trans; 1.
DR   Pfam; PF01702; TGT; 1.
DR   TIGRFAMs; TIGR00430; Q_tRNA_tgt; 1.
DR   TIGRFAMs; TIGR00449; tgt_general; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Glycosyltransferase; Metal-binding;
KW   Queuosine biosynthesis; Transferase; tRNA processing; Zinc.
FT   CHAIN         1    374       Queuine tRNA-ribosyltransferase.
FT                                /FTId=PRO_1000016769.
FT   ACT_SITE     90     90       Nucleophile (By similarity).
FT   METAL       308    308       Zinc (By similarity).
FT   METAL       310    310       Zinc (By similarity).
FT   METAL       313    313       Zinc (By similarity).
FT   METAL       339    339       Zinc (By similarity).
FT   BINDING      91     91       Substrate (By similarity).
SQ   SEQUENCE   374 AA;  42297 MW;  0A48196FCBAEE4EB CRC64;
     MNFKIDKTDG NARACTLQTA HSTIQTPIFM PVGTLGAVKS LDAIDLKEIL DAKIILANTY
     HLYLRPTSKV VREFGGLHGF SKFDRSFLTD SGGFQAFSLS KISKPDENGI KFKSHIDGSM
     HYFTPKSVLD TQYDLSSDIM MILDDLVALP ATKERIDLSI KRTINWAKIA CEYHKSNKQK
     SVGIDQNIFG IIQGGTDYNA RKLCAEALCE MEFDGLAIGG LSVGESNEEM YDTVEALMPF
     IDKNRPRYLM GVGTPEDLVQ NVERGVDMFD CVMPTRNARN GTLFTSFGKI NIKSAAFIKD
     DNKIDPECDC YTCSNFSRGY LNHLYKAREL TFFRLASLHN LHYYLNLVKQ MREAIMQGKF
     KEFKREFYAK RGMI
//