Reviewed, UniProtKB/Swiss-Prot A0RPL5 (TGT_CAMFF)

Names and origin

Protein names

Recommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme

Gene names

Name:	tgt
Ordered Locus Names:	CFF8240_0980

Organism

Campylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]

Taxonomic identifier

360106 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000016769
Sites
	Active site	90	1	Nucleophile By similarity
	Metal binding	308	1	Zinc By similarity
	Metal binding	310	1	Zinc By similarity
	Metal binding	313	1	Zinc By similarity
	Metal binding	339	1	Zinc By similarity
	Binding site	91	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A0RPL5-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 0A48196FCBAEE4EB	FASTA	374	42,297
	10 20 30 40 50 60 MNFKIDKTDG NARACTLQTA HSTIQTPIFM PVGTLGAVKS LDAIDLKEIL DAKIILANTY 70 80 90 100 110 120 HLYLRPTSKV VREFGGLHGF SKFDRSFLTD SGGFQAFSLS KISKPDENGI KFKSHIDGSM 130 140 150 160 170 180 HYFTPKSVLD TQYDLSSDIM MILDDLVALP ATKERIDLSI KRTINWAKIA CEYHKSNKQK 190 200 210 220 230 240 SVGIDQNIFG IIQGGTDYNA RKLCAEALCE MEFDGLAIGG LSVGESNEEM YDTVEALMPF 250 260 270 280 290 300 IDKNRPRYLM GVGTPEDLVQ NVERGVDMFD CVMPTRNARN GTLFTSFGKI NIKSAAFIKD 310 320 330 340 350 360 DNKIDPECDC YTCSNFSRGY LNHLYKAREL TFFRLASLHN LHYYLNLVKQ MREAIMQGKF 370 KEFKREFYAK RGMI

References

[1]	"Sequence of Campylobacter fetus subsp. fetus 82-40." Fouts D.E., Nelson K.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000487 Genomic DNA. Translation: ABK81825.1.
RefSeq	YP_892148.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4537752.
GenomeReviews	Gene locus CFF8240_0980 in contig CP000487_GR.
KEGG	cff:CFF8240_0980.
NMPDR	fig|360106.5.peg.934.
TIGR	CFF8240_0980.
Phylogenomic databases
OMA	A0RPL5. ECVRLPA.
Family and domain databases
HAMAP	MF_00168. [Tree]
InterPro	IPR004803. QtRNA_ribo_trans. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_CAMFF

Accession

Primary (citable) accession number: A0RPL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)