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Protein

UvrABC system protein B

Gene

uvrB

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:CFF8240_0871
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000760 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000778711 – 658UvrABC system protein BAdd BLAST658

Proteomic databases

PRIDEiA0RPB3.

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0RPB3.
SMRiA0RPB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 182Helicase ATP-bindingUniRule annotationAdd BLAST158
Domaini433 – 596Helicase C-terminalUniRule annotationAdd BLAST164
Domaini623 – 658UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 114Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

A0RPB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKFEISSKF KPSDDQEKAV TNIVDSIRSG NKFNVLLGVT GSGKTFTMAN
60 70 80 90 100
VIKRLNMPTL IMTHNKSLAA QLYSEFKGFF PKNHVEYFIS YYDYYQPEAY
110 120 130 140 150
IPRQDLFIEK DSSINEELER LRLSATANLL EFDDVVVVAS VSANYGLGNP
160 170 180 190 200
AEYKGMVLLL SLGMSLNQKE LLLKLVDMGY KRNDAYFDRG DFRVNGDVVD
210 220 230 240 250
IYPAYFNDEA IRLEFFGDEL DAMYHFDVLE NKRTKDVSKF ILYATSQFIV
260 270 280 290 300
GENRLKQAIK DIELELEDRL AFYEKENRLV EYQRLKQRVE FDLEMLSSTG
310 320 330 340 350
STKGVENYAR YLTGQKAGET PYSLFDYFEV SGKDYLVIVD ESHVSLPQFR
360 370 380 390 400
GMYAGDRSRK EVLVEYGFRL PSALDNRPLK LDEFIAKKAN YLFVSATPNQ
410 420 430 440 450
YEIDLSQGHV YEQILRPTGL LDPRIEVISS DNQVEVLFDR AKAVIARGER
460 470 480 490 500
VLVTTLTKKM SEELTRYYQE LGIKVKYMHS DIDAVERNEL IRGLRKGEFD
510 520 530 540 550
MLVGINLLRE GLDLPEVSLV AVLDADKEGF LRSRTSLIQT MGRAARNVNG
560 570 580 590 600
TVILFANKIT NSMKEAIDTT EARRKYQGDY NKKYGITPRS ASRNLEDSLK
610 620 630 640 650
EEDLPNLYNK AKKLEKMPAS ERAKIVKELR KQMLEAAKNL EFEKAAALRD

EIAKLREL
Length:658
Mass (Da):75,496
Last modified:January 9, 2007 - v1
Checksum:i9AD634A57034734E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK83001.1.
RefSeqiWP_002849372.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK83001; ABK83001; CFF8240_0871.
KEGGicff:CFF8240_0871.

Similar proteinsi

Entry informationi

Entry nameiUVRB_CAMFF
AccessioniPrimary (citable) accession number: A0RPB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 9, 2007
Last modified: October 25, 2017
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families