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A0RNX3 (SYL_CAMFF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CFF8240_0731
OrganismCampylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]
Taxonomic identifier360106 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length811 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 811811Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009316

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif579 – 5835"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A0RNX3 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 7A2CFDF0E6B32A25

FASTA81193,325
        10         20         30         40         50         60 
MEYNAKNIEY KWQQIWKKNG YSEPKDDYSL PKKYILSMFP YPSGRLHMGH VRNYSIGDAL 

        70         80         90        100        110        120 
SRYYRNRGYN VLQPIGFDSF GMPAENAAIK HKIHPKIWTY DNIDYMTKEL DALGFSFSKK 

       130        140        150        160        170        180 
RLFATSDPLY TRWEQEFFIR MYEKGLVYRK SAVVNWCEND QTVLANEQVE DGKCWRCGHE 

       190        200        210        220        230        240 
VIQKEMPGYY LKITDYANEL LECLKDLEGK WPNQVLTMQE NWIGKSYGLE FEFKFDSSSK 

       250        260        270        280        290        300 
ILLDGMDGFK VFTTRPDTIY GVSYAAIAPE HIVVKKLLEK NILDDATSAK LKFILNQSPK 

       310        320        330        340        350        360 
QRQSIDKDGV SLGLNVIHPL TNELIPVWCA NFVLAEYGGG AVMSVPAHDE RDFEFASKFN 

       370        380        390        400        410        420 
LNIKQIIKSD TLPYCEKSGV YINSELINGL AYEEAREKII SKFEKEGWGN RVTNYKLRDW 

       430        440        450        460        470        480 
GISRQRYWGA PIPMIHCKKC GVVPENISNL PVKLPDDVVI TGEGNPLDKH SEFKNCKCPK 

       490        500        510        520        530        540 
CGSQATRETD TMDTFFESSW YFARFASDEK TWEDVAFDKK SVDYWMSVDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYARFFQK ALRDLGYLRD CEPFDSLLTQ GMVLKDGSKM SKSKGNTVDP DDIINKFGAD 

       610        620        630        640        650        660 
TARLFILFAA PPQKELEWND SAVEGAFKFI NRLYDRSDNA YKTEILPQIN HSNLNKDEKY 

       670        680        690        700        710        720 
ARLKVYEALK KSTDVFENSF AFNTLIAACM EALNGLNAQN NKDIWTEGYF IILNLLEPII 

       730        740        750        760        770        780 
PHACHELSNE LFGLKNFTKL SLKDEVFVKD SLNLAITVNG KRRSEIEVSA FESDDKILEI 

       790        800        810 
AKQEVSKWIE GKEILKEIYV PNKLVNLVIK G 

« Hide

References

[1]"Sequence of Campylobacter fetus subsp. fetus 82-40."
Fouts D.E., Nelson K.E.
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 82-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000487 Genomic DNA. Translation: ABK82789.1.
RefSeqYP_891906.1. NC_008599.1.

3D structure databases

ProteinModelPortalA0RNX3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING360106.CFF8240_0731.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABK82789; ABK82789; CFF8240_0731.
GeneID4539205.
KEGGcff:CFF8240_0731.
PATRIC20035978. VBICamFet25865_0705.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCFET360106:GHTH-730-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAMFF
AccessionPrimary (citable) accession number: A0RNX3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries