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A0RNK3

- SYI_CAMFF

UniProt

A0RNK3 - SYI_CAMFF

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi891 – 8911ZincUniRule annotation
    Metal bindingi894 – 8941ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCFET360106:GHTH-609-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CFF8240_0610
    OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
    Taxonomic identifieri360106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000760: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 916916Isoleucine--tRNA ligasePRO_1000022054Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360106.CFF8240_0610.

    Structurei

    3D structure databases

    ProteinModelPortaliA0RNK3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A0RNK3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKDTLLLP TTDFAMRGNL PECEPARYAK WDEQKVYEKM KAKREKSAIS    50
    FNIHDGPPYA NGHLHIGHAL NKILKDIILK THYFFGQGIR YTPGWDCHGL 100
    PIEQQVEVKL GDKKKSMSKS DIRQECRNWA KEFITVQKDE FKSLGVIGDW 150
    NDPYLTMKFK FEANIYRTLC EVAKKGLLVE RSKPVFWSWA ARSALAEAEV 200
    EYEDKEDYSL YVAFCLSDAA CKKLGVSDAK AVIWTTTPWT LVANQAISLN 250
    PNEKYALTSE GYIIALPLKE TLINQGIING KILKEFASSE LEGLSAINPL 300
    NGRDSRFILG EHVLMDGGTG LVHTAPGHGE DDYFASLKYG IEVIMPVDEA 350
    GLYDETLRVK KLLPEHLLNE FIGLHIFKAN EKLIELLGDA AVKVSKFVHS 400
    YPFCWRTHKP VIYRATKQWF IAMDEPKLSG KTLRQTALEA LKSVKFYPEV 450
    GVKRISSMIE NRPDWCISRQ RDWGVPIAFF RDSATKEPIF DSRVLEHVAK 500
    VFDEKGSDAW WNLEISELLP KGTNLDPSKL EKVTDILDVW FDSGSTWNAV 550
    LNSGDYDAGG YQADMYLEGS DQHRGWFQSS LLLSCAINQK APYKSVLTHG 600
    FTVDGNGNKM SKSKGNVIAP SDVAKRYGVE ILRLWVGLSD YSSDLKISDD 650
    ILKQVAEQYR KIRNTIRFLL ANINDLDDIS SHFGTLDKWI LARATKSFKE 700
    VSECFKNYEY SKGFNILLNF LSTDLSGIYL DICKDRLYCD EKDGLRRRSS 750
    QSAMAIIAKS LLSLIAPTLT YTVDEAIEFA PDIIKNGAKD AFDLVYEPLV 800
    FEFKIDDELL TSSREKFNEI IDVLKKDKII KSTLEVILET SSNEILANDL 850
    DEIIDWYMVS FVRNIESDEF LAEFAVGNDK FKIVKADRYK CPRCWKYAAK 900
    IDNDLCPRCA KVLKRV 916
    Length:916
    Mass (Da):104,180
    Last modified:January 9, 2007 - v1
    Checksum:i9A053BBF79057565
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000487 Genomic DNA. Translation: ABK82625.1.
    RefSeqiYP_891786.1. NC_008599.1.

    Genome annotation databases

    EnsemblBacteriaiABK82625; ABK82625; CFF8240_0610.
    GeneIDi4537860.
    KEGGicff:CFF8240_0610.
    PATRICi20035742. VBICamFet25865_0588.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000487 Genomic DNA. Translation: ABK82625.1 .
    RefSeqi YP_891786.1. NC_008599.1.

    3D structure databases

    ProteinModelPortali A0RNK3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360106.CFF8240_0610.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK82625 ; ABK82625 ; CFF8240_0610 .
    GeneIDi 4537860.
    KEGGi cff:CFF8240_0610.
    PATRICi 20035742. VBICamFet25865_0588.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CFET360106:GHTH-609-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
      Fouts D.E., Nelson K.E.
      Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 82-40.

    Entry informationi

    Entry nameiSYI_CAMFF
    AccessioniPrimary (citable) accession number: A0RNK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3