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A0RNK3

- SYI_CAMFF

UniProt

A0RNK3 - SYI_CAMFF

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi891 – 8911ZincUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCFET360106:GHTH-609-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CFF8240_0610
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000760: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 916916Isoleucine--tRNA ligasePRO_1000022054Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi360106.CFF8240_0610.

Structurei

3D structure databases

ProteinModelPortaliA0RNK3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RNK3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTLLLP TTDFAMRGNL PECEPARYAK WDEQKVYEKM KAKREKSAIS
60 70 80 90 100
FNIHDGPPYA NGHLHIGHAL NKILKDIILK THYFFGQGIR YTPGWDCHGL
110 120 130 140 150
PIEQQVEVKL GDKKKSMSKS DIRQECRNWA KEFITVQKDE FKSLGVIGDW
160 170 180 190 200
NDPYLTMKFK FEANIYRTLC EVAKKGLLVE RSKPVFWSWA ARSALAEAEV
210 220 230 240 250
EYEDKEDYSL YVAFCLSDAA CKKLGVSDAK AVIWTTTPWT LVANQAISLN
260 270 280 290 300
PNEKYALTSE GYIIALPLKE TLINQGIING KILKEFASSE LEGLSAINPL
310 320 330 340 350
NGRDSRFILG EHVLMDGGTG LVHTAPGHGE DDYFASLKYG IEVIMPVDEA
360 370 380 390 400
GLYDETLRVK KLLPEHLLNE FIGLHIFKAN EKLIELLGDA AVKVSKFVHS
410 420 430 440 450
YPFCWRTHKP VIYRATKQWF IAMDEPKLSG KTLRQTALEA LKSVKFYPEV
460 470 480 490 500
GVKRISSMIE NRPDWCISRQ RDWGVPIAFF RDSATKEPIF DSRVLEHVAK
510 520 530 540 550
VFDEKGSDAW WNLEISELLP KGTNLDPSKL EKVTDILDVW FDSGSTWNAV
560 570 580 590 600
LNSGDYDAGG YQADMYLEGS DQHRGWFQSS LLLSCAINQK APYKSVLTHG
610 620 630 640 650
FTVDGNGNKM SKSKGNVIAP SDVAKRYGVE ILRLWVGLSD YSSDLKISDD
660 670 680 690 700
ILKQVAEQYR KIRNTIRFLL ANINDLDDIS SHFGTLDKWI LARATKSFKE
710 720 730 740 750
VSECFKNYEY SKGFNILLNF LSTDLSGIYL DICKDRLYCD EKDGLRRRSS
760 770 780 790 800
QSAMAIIAKS LLSLIAPTLT YTVDEAIEFA PDIIKNGAKD AFDLVYEPLV
810 820 830 840 850
FEFKIDDELL TSSREKFNEI IDVLKKDKII KSTLEVILET SSNEILANDL
860 870 880 890 900
DEIIDWYMVS FVRNIESDEF LAEFAVGNDK FKIVKADRYK CPRCWKYAAK
910
IDNDLCPRCA KVLKRV
Length:916
Mass (Da):104,180
Last modified:January 9, 2007 - v1
Checksum:i9A053BBF79057565
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82625.1.
RefSeqiWP_011731879.1. NC_008599.1.
YP_891786.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK82625; ABK82625; CFF8240_0610.
GeneIDi4537860.
KEGGicff:CFF8240_0610.
PATRICi20035742. VBICamFet25865_0588.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82625.1 .
RefSeqi WP_011731879.1. NC_008599.1.
YP_891786.1. NC_008599.1.

3D structure databases

ProteinModelPortali A0RNK3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 360106.CFF8240_0610.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABK82625 ; ABK82625 ; CFF8240_0610 .
GeneIDi 4537860.
KEGGi cff:CFF8240_0610.
PATRICi 20035742. VBICamFet25865_0588.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CFET360106:GHTH-609-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
    Fouts D.E., Nelson K.E.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 82-40.

Entry informationi

Entry nameiSYI_CAMFF
AccessioniPrimary (citable) accession number: A0RNK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3