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A0RN28 (ISPDF_CAMFF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional enzyme IspD/IspF

Including the following 2 domains:

  1. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
    EC=2.7.7.60
    Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase
    MEP cytidylyltransferase
    Short name=MCT
  2. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
    Short name=MECDP-synthase
    Short name=MECPS
    EC=4.6.1.12
Gene names
Name:ispDF
Ordered Locus Names:CFF8240_0409
OrganismCampylobacter fetus subsp. fetus (strain 82-40) [Complete proteome] [HAMAP]
Taxonomic identifier360106 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) By similarity. HAMAP MF_01520

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520

Cofactor

Divalent metal cations By similarity. HAMAP MF_01520

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.

Sequence similarities

In the N-terminal section; belongs to the IspD family.

In the C-terminal section; belongs to the IspF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Bifunctional enzyme IspD/IspF HAMAP MF_01520
PRO_0000296741

Regions

Region1 – 2102102-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
Region211 – 3721622-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520

Sites

Metal binding2171Divalent metal cation By similarity
Metal binding2191Divalent metal cation By similarity
Metal binding2511Divalent metal cation By similarity
Site161Transition state stabilizer By similarity
Site231Transition state stabilizer By similarity
Site1391Positions MEP for the nucleophilic attack By similarity
Site1911Positions MEP for the nucleophilic attack By similarity
Site2431Transition state stabilizer By similarity
Site3421Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A0RN28 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 06919DE8555C8335

FASTA37241,387
        10         20         30         40         50         60 
MLDLSLIMLG AGNSTRFGLQ SKKQWLRTGD DPLWLYATKN ISSNYLFKDV IVVSNECEYM 

        70         80         90        100        110        120 
RKFSSHFKFI KGGETRQDSL RNAISNINSE FVMVSDIARA DIPKELITKL IESAHNADCI 

       130        140        150        160        170        180 
VPALKISDSV IYQNEYINRD ELKLIQTPQL SRTNMLKKAL KTDNIFTDDS SAIKAIGGTV 

       190        200        210        220        230        240 
WYIEGDERAK KLTYKDDLKR LNLNSPSNDI FCGNGFDVHA FKEGDFITLC GVKIPYSKAF 

       250        260        270        280        290        300 
IAHSDGDVAL HALCDALLGA ASAPDIGELY PDNDSKFKDI DSKILLQNSV NLIRSIGFDI 

       310        320        330        340        350        360 
INADITIIAQ SPKISPYKDA MAKIVADILG IPLHKVNIKA TTTEHLGFIG RNEGIAANAI 

       370 
VNLKYFNWRN VL

« Hide

References

[1]"Sequence of Campylobacter fetus subsp. fetus 82-40."
Fouts D.E., Nelson K.E.
Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 82-40.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000487 Genomic DNA. Translation: ABK81838.1.
RefSeqYP_891611.1. NC_008599.1.

3D structure databases

ProteinModelPortalA0RN28.
SMRA0RN28. Positions 3-370.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0RN28.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4537765.
GenomeReviewsGene locus CFF8240_0409 in contig CP000487_GR.
KEGGcff:CFF8240_0409.
NMPDRfig|360106.5.peg.396.
PATRIC20035344. VBICamFet25865_0404.
TIGRCFF8240_0409.

Phylogenomic databases

eggNOGCOG1211.
HOGENOMHBG672839.
OMAIVLIHDA.
ProtClustDBPRK09382.

Enzyme and pathway databases

BioCycCFET360106:CFF8240_0409-MONOMER.

Family and domain databases

HAMAPMF_01520. IspDF.
[Tree]
InterProIPR023423. IpsF_dom.
IPR001228. ISPD_synthase.
IPR003526. MECDP_synthase.
IPR020555. MECDP_synthase_CS.
[Graphical view]
Gene3DG3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
KOK12506.
PfamPF01128. IspD. 1 hit.
PF02542. YgbB. 1 hit.
[Graphical view]
SUPFAMSSF69765. YgbB_synth. 1 hit.
TIGRFAMsTIGR00453. IspD. 1 hit.
TIGR00151. IspF. 1 hit.
PROSITEPS01295. ISPD. False negative.
PS01350. ISPF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPDF_CAMFF
AccessionPrimary (citable) accession number: A0RN28
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: January 9, 2007
Last modified: January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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