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A0RME8

- BIOB_CAMFF

UniProt

A0RME8 - BIOB_CAMFF

Protein

Biotin synthase

Gene

bioB

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi13 – 131Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi17 – 171Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi20 – 201Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi57 – 571Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi92 – 921Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi150 – 1501Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi217 – 2171Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCFET360106:GHTH-176-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:CFF8240_0176
    OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
    Taxonomic identifieri360106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000760: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275Biotin synthasePRO_0000381282Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi360106.CFF8240_0176.

    Structurei

    3D structure databases

    ProteinModelPortaliA0RME8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A0RME8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLCAICNVSS GNCAEDCAYC TQSAHIKADI PKFKQKNLEQ ILNEAKIASK    50
    NYALGFCLVT SGLGLDDKKL EFICEAATML RKETPNLMLI ACNGSASYES 100
    LKELKKVGIF SYNHNLETSR EFFPQICTTH SWDDRFNTNL NAKKAGLELC 150
    CGGIYGLGES QDDRLSFRAS LKELNPFSSP INFFIPNPAL KIKQPLLSTD 200
    EALEIIRDTA KTLPQCRIMV AGGREIVLGD RQYEILENGA SAIVIGDYLT 250
    TSGEVASKDI EELRNRGFEF ASQCH 275
    Length:275
    Mass (Da):30,401
    Last modified:January 9, 2007 - v1
    Checksum:i4C2A39A4858571CB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000487 Genomic DNA. Translation: ABK82310.1.
    RefSeqiWP_011731724.1. NC_008599.1.
    YP_891381.1. NC_008599.1.

    Genome annotation databases

    EnsemblBacteriaiABK82310; ABK82310; CFF8240_0176.
    GeneIDi4539412.
    KEGGicff:CFF8240_0176.
    PATRICi20034880. VBICamFet25865_0173.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000487 Genomic DNA. Translation: ABK82310.1 .
    RefSeqi WP_011731724.1. NC_008599.1.
    YP_891381.1. NC_008599.1.

    3D structure databases

    ProteinModelPortali A0RME8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 360106.CFF8240_0176.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK82310 ; ABK82310 ; CFF8240_0176 .
    GeneIDi 4539412.
    KEGGi cff:CFF8240_0176.
    PATRICi 20034880. VBICamFet25865_0173.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci CFET360106:GHTH-176-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
      Fouts D.E., Nelson K.E.
      Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 82-40.

    Entry informationi

    Entry nameiBIOB_CAMFF
    AccessioniPrimary (citable) accession number: A0RME8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3