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Protein

Biotin synthase

Gene

bioB

Organism
Campylobacter fetus subsp. fetus (strain 82-40)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi17 – 171Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi20 – 201Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi57 – 571Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi92 – 921Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi150 – 1501Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi217 – 2171Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCFET360106:GHTH-176-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:CFF8240_0176
OrganismiCampylobacter fetus subsp. fetus (strain 82-40)
Taxonomic identifieri360106 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000760: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Biotin synthasePRO_0000381282Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi360106.CFF8240_0176.

Structurei

3D structure databases

ProteinModelPortaliA0RME8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiYNINIQV.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A0RME8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLCAICNVSS GNCAEDCAYC TQSAHIKADI PKFKQKNLEQ ILNEAKIASK
60 70 80 90 100
NYALGFCLVT SGLGLDDKKL EFICEAATML RKETPNLMLI ACNGSASYES
110 120 130 140 150
LKELKKVGIF SYNHNLETSR EFFPQICTTH SWDDRFNTNL NAKKAGLELC
160 170 180 190 200
CGGIYGLGES QDDRLSFRAS LKELNPFSSP INFFIPNPAL KIKQPLLSTD
210 220 230 240 250
EALEIIRDTA KTLPQCRIMV AGGREIVLGD RQYEILENGA SAIVIGDYLT
260 270
TSGEVASKDI EELRNRGFEF ASQCH
Length:275
Mass (Da):30,401
Last modified:January 9, 2007 - v1
Checksum:i4C2A39A4858571CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82310.1.
RefSeqiWP_011731724.1. NC_008599.1.
YP_891381.1. NC_008599.1.

Genome annotation databases

EnsemblBacteriaiABK82310; ABK82310; CFF8240_0176.
GeneIDi4539412.
KEGGicff:CFF8240_0176.
PATRICi20034880. VBICamFet25865_0173.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000487 Genomic DNA. Translation: ABK82310.1.
RefSeqiWP_011731724.1. NC_008599.1.
YP_891381.1. NC_008599.1.

3D structure databases

ProteinModelPortaliA0RME8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi360106.CFF8240_0176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK82310; ABK82310; CFF8240_0176.
GeneIDi4539412.
KEGGicff:CFF8240_0176.
PATRICi20034880. VBICamFet25865_0173.

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiYNINIQV.
OrthoDBiEOG622PMP.

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.
BioCyciCFET360106:GHTH-176-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Sequence of Campylobacter fetus subsp. fetus 82-40."
    Fouts D.E., Nelson K.E.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 82-40.

Entry informationi

Entry nameiBIOB_CAMFF
AccessioniPrimary (citable) accession number: A0RME8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 9, 2007
Last modified: January 7, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.