Reviewed, UniProtKB/Swiss-Prot A0RJS6 (MURC_BACAH)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BALH_4262

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004310
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A0RJS6-1 [UniParc]. Last modified January 9, 2007. Version 1. Checksum: 10C9536793B9FE4F	FASTA	436	49,257
	10 20 30 40 50 60 MTVYHFVGIK GTGMSSLAQI LHDMKHTVQG SDYEKRFFTQ TALEKRNISI LPFDKSNVKE 70 80 90 100 110 120 GQVIIAGNAF PDTHEEIVAA KELNIPVHRY HHFLGDLMNQ YTSVAVTGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQGAHPTS YLIGDGTGHG VENSKYFVFE ACEYRRHFLS YNPDYAIMTN IDFDHPDYFT 190 200 210 220 230 240 DINDVFSAFQ EMALQVKKGI IACGDDEELQ KIQAKVPVIF YGFGEDNDFQ ARNIQKRTDG 250 260 270 280 290 300 TIFDVFVRNT YYDTFKITGY GNHSVLNALA VIALCHYENV DVEAVKHQLT TFEGVKRRFN 310 320 330 340 350 360 EKPMGEQVII DDYAHHPTEI NATIEAARQK HPEREIVAVF QPHTFSRTEK FLDEFAESLS 370 380 390 400 410 420 KADQVYLCDI FGSARENKGE LTIEDLQKRI DGAELITDTT TDVLKKHKNG VLIFMGAGDI 430 QKFEAAYVKE VQVAEK

References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam." Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S. , Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., Martinez D., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Gilna P., Brettin T.S. J. Bacteriol. 189:3680-3681(2007) [PubMed: 17337577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000485 Genomic DNA. Translation: ABK87469.1.
RefSeq	YP_896976.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A0RJS6.
Genome annotation databases
GeneID	4543793.
GenomeReviews	Gene locus BALH_4262 in contig CP000485_GR.
KEGG	btl:BALH_4262.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACAH

Accession

Primary (citable) accession number: A0RJS6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 9, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)