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Protein

Argininosuccinate synthase

Gene

argG

Organism
Bacillus thuringiensis (strain Al Hakam)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF), Ornithine carbamoyltransferase (arcB), Ornithine carbamoyltransferase (argF), Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG), Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH), Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86CitrullineUniRule annotation1
Binding sitei116ATP; via amide nitrogenUniRule annotation1
Binding sitei118AspartateUniRule annotation1
Binding sitei122AspartateUniRule annotation1
Binding sitei122CitrullineUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei126CitrullineUniRule annotation1
Binding sitei174CitrullineUniRule annotation1
Binding sitei183CitrullineUniRule annotation1
Binding sitei259CitrullineUniRule annotation1
Binding sitei271CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 17ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:BALH_4210
OrganismiBacillus thuringiensis (strain Al Hakam)
Taxonomic identifieri412694 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000761 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003213021 – 401Argininosuccinate synthaseAdd BLAST401

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA0RJM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A0RJM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKKVVLAY SGGLDTSVAI KWLQEKNYDI IALCLDLGEG KDLAFVKEKA
60 70 80 90 100
LSVGAIKSYM IDVQEEFANE YALMAMQAHT LYEGKYPLVS ALSRPLIAKK
110 120 130 140 150
LVEIAEQEGA TAVAHGCTGK GNDQVRFEVS IQALNPYLEV IAPVREWKWS
160 170 180 190 200
REEEIAYAKE NNVPIPINLD SPFSIDQNLW GRSNECGILE DPWAAPPEDA
210 220 230 240 250
YEMTLALEDT PNKPEFVEIG FEAGVPTTLN GTAYPLSELI KTLNALAGKH
260 270 280 290 300
GVGRIDHVEN RLVGIKSREV YECPAAMTLI TAHKELEDLT LVKEVAHFKP
310 320 330 340 350
MIEQKITELI YNGLWFSPLK QALNAFLQET QKNVTGTVRV KLFKGHAIVE
360 370 380 390 400
GRKSEYSLYD EKLATYTAQD EFNHDAAVGF ISLFGLPTKV YSQVNQKKVE

A
Length:401
Mass (Da):44,670
Last modified:February 26, 2008 - v2
Checksum:iD3E21C76DC5D4AD0
GO

Sequence cautioni

The sequence ABK87417 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA. Translation: ABK87417.1. Different initiation.
RefSeqiWP_000412329.1. NC_008600.1.

Genome annotation databases

EnsemblBacteriaiABK87417; ABK87417; BALH_4210.
KEGGibtl:BALH_4210.
PATRICi19001346. VBIBacThu63319_4669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000485 Genomic DNA. Translation: ABK87417.1. Different initiation.
RefSeqiWP_000412329.1. NC_008600.1.

3D structure databases

ProteinModelPortaliA0RJM4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK87417; ABK87417; BALH_4210.
KEGGibtl:BALH_4210.
PATRICi19001346. VBIBacThu63319_4669.

Phylogenomic databases

HOGENOMiHOG000230093.
KOiK01940.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_BACAH
AccessioniPrimary (citable) accession number: A0RJM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: November 2, 2016
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.