Heme-degrading monooxygenase - Bacillus thuringiensis (strain Al Hakam)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Heme-degrading monooxygenase
EC=1.14.99.3

Alternative name(s):
Heme oxygenase
Iron-regulated surface determinant
Iron-responsive surface determinant

Gene names

Name:	isdG
Ordered Locus Names:	BALH_4126

Organism

Bacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]

Taxonomic identifier

412694 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group ›

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the biliverdin in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron By similarity. HAMAP-Rule MF_01272
Catalytic activity	Heme + 3 AH₂ + 3 O₂ = biliverdin + Fe²⁺ + CO + 3 A + 3 H₂O. HAMAP-Rule MF_01272
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	heme catabolic process Inferred from electronic annotation. Source: HAMAP iron assimilation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heme binding Inferred from electronic annotation. Source: HAMAP heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 107

107

Heme-degrading monooxygenase HAMAP-Rule MF_01272

PRO_1000067382

Sites

Metal binding

6

1

Iron By similarity

Metal binding

76

1

Iron (heme axial ligand) By similarity

Site

66

1

Transition state stabilizer By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A0RJE3 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: ACB308A337A92DFB
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FASTA

107

12,004

        10         20         30         40         50         60 
MIIVTNTAKI TKGNGHKLID RFNKVGQVET MPGFLGLEVL LTQNTVDYDE VTISTRWNAK 

        70         80         90        100 
EDFQGWTKSP AFKAAHSHQG GMPDYILDNK ISYYDVKVVR MPMAAAQ

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References

[1]

"The complete genome sequence of Bacillus thuringiensis Al Hakam."
Challacombe J.F., Altherr M.R., Xie G., Bhotika S.S., Brown N., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Green L.D., Han C.S.

Brettin T.S.
J. Bacteriol. 189:3680-3681(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Al Hakam.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000485 Genomic DNA. Translation: ABK87336.1.
RefSeq	YP_896843.1. NC_008600.1.
3D structure databases
ProteinModelPortal	A0RJE3.
ModBase	Search...
Protein-protein interaction databases
STRING	412694.BALH_4126.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABK87336; ABK87336; BALH_4126.
GeneID	4544515.
KEGG	btl:BALH_4126.
PATRIC	19001150. VBIBacThu63319_4571.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2329.
HOGENOM	HOG000008026.
KO	K07145.
OMA	VTNTSKI.
ProtClustDB	PRK13314.
Enzyme and pathway databases
BioCyc	BTHU412694:GH1W-3917-MONOMER.
Family and domain databases
HAMAP	MF_01272. Heme_degrading_monooxygenase.
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. IPR023953. Heme-degrad_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HDOX_BACAH

Accession

Primary (citable) accession number: A0RJE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents