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A0RJ24 (TGT_BACAH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:BALH_3997
OrganismBacillus thuringiensis (strain Al Hakam) [Complete proteome] [HAMAP]
Taxonomic identifier412694 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_1000016764

Sites

Active site941Nucleophile By similarity
Metal binding3061Zinc By similarity
Metal binding3081Zinc By similarity
Metal binding3111Zinc By similarity
Metal binding3371Zinc By similarity
Binding site951Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A0RJ24 [UniParc].

Last modified January 9, 2007. Version 1.
Checksum: 6DE1E29B88568644

FASTA37943,227
        10         20         30         40         50         60 
MTAIRYEFIK TCKQTGARLG RVHTPHGSFD TPTFMPVGTL ATVKTMSPEE LKAMDSGIIL 

        70         80         90        100        110        120 
SNTYHLWLRP GHEIIREAGG LHKFMNWDRA ILTDSGGFQV FSLSDFRRIE EEGVHFRNHL 

       130        140        150        160        170        180 
NGDKLFLSPE KAMEIQNALG SDIMMAFDEC PPFPATFEYM KKSVERTSRW AERCLKAHER 

       190        200        210        220        230        240 
PQDQGLFGIV QGGEFEELRR QSAKDLVSMD FPGYAVGGLS VGEPKDIMNR VLEFTTPLLP 

       250        260        270        280        290        300 
DNKPRYLMGV GSPDSLIDGA IRGIDMFDCV LPTRIARNGT CMTSEGRLVV KNAKFARDFG 

       310        320        330        340        350        360 
PLDPNCDCYT CKNYSRAYIR HLMKCDETFG IRLTSYHNLH FLLNLMEQVR QAIREDRLGD 

       370 
FREEFFEQYG FNKPNAKNF

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000485 Genomic DNA. Translation: ABK87217.1.
RefSeqYP_896724.1. NC_008600.1.

3D structure databases

ProteinModelPortalA0RJ24.
SMRA0RJ24. Positions 5-369.
ModBaseSearch...

Protein-protein interaction databases

STRINGA0RJ24.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000067741; EBBACP00000065978; EBBACG00000067732.
GeneID4543572.
GenomeReviewsGene locus BALH_3997 in contig CP000485_GR.
KEGGbtl:BALH_3997.
PATRIC19000881. VBIBacThu63319_4438.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0343.
GeneTreeEBGT00050000001431.
HOGENOMHBG629929.
OMAFMPVGTV.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycBTHU412694:BALH_3997-MONOMER.

Family and domain databases

HAMAPMF_00168. Q_tRNA_Tgt.
[Tree]
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KOK00773.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. Tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_BACAH
AccessionPrimary (citable) accession number: A0RJ24
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 9, 2007
Last modified: December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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